Chlorpyrifos –Induced Clinical, Hematological and Bio-chemical Changes in Swiss-Albino Mice-Mitigating effect by Co-administration of Vitamin C and E
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Date
2010
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Published by Zhengzhou University
Abstract
Background. Induction of Oxidative stress is one of the molecular mechanisms in chlorpyrifos toxicity.Objective.
To evaluate the effect of prolonged CPF exposure on clinical, hematological and biochemical parameters in mice and the possible ameliorative effect of coadministration of vitamins C and E. Methods. 40 mice divided into 4 groups of 10 animals in each group served as subjects for this study. Groups I and II were administered corn oil (2 ml/kg) and combination of vitamins C (100 mg/kg) and E (75 mg/kg), respectively. Group III were exposed to CPF only (21.6 mg/kg ~ 1/5th of the previously determined LD50 of 108 mg/kg), while group IV were pretreated with combination of vitamins C (100 mg/kg) and E (75 mg/kg) and then administered CPF (21.6 mg/kg) 30 min later. The regimens were administered orally once daily for a period of 10 weeks. The mice were examined for signs of toxicity and weekly body weight changes. Blood and serum samples obtained from sacrificed animals at the end of the study were evaluated for some hematological and biochemical parameters, respectively. Results.Vitamins pretreatment ameliorated cholinergic toxic signs and changes in body weight,
PCV, Hb, RBC and WBC count induced by CPF. CPF-evoked alteration in Na+, K+, Cl-, TP, urea, creatinine, ALP and MDA levels were ameliorated by pretreatment with the vitamins. ALT and AST activities lowered by CPF was further reduced by vitamins pretreatment. Conclusion. Vitamins C and E protected mice from subchronic CPF-induced alteration in clinical,hematological and serum biochemical parameters.
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Keywords
Chlorpyrifos;, hematology;, serum biochemistry,, vitamins C and E., lipid peroxidation
Citation
Ambali SF, Akanbi, DO, Shittu M, Giwa A, Oladipo OO and Ayo, JO (2010): Chlorpyrifos –Induced Clinical, Hematological and Bio-chemical Changes in Swiss-Albino Mice-Mitigating effect by Co-administration of Vitamin C and E. Life Science Journal, 7(3); 37-44. Published by Zhengzhou University