Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid.
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Date
2012
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Publisher
Ondersterpoort Veterinary Institute
Abstract
Experiments were performed with the aim of investigating the effect of packing on erythrocyte
osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the
effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional
extensive system served as experimental subjects. Six donkeys administered orally with AA
(200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not
administered with AA served as controls. Blood samples were collected pre- and post-packing
from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl)
concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the
value was significantly (P < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the
experimental donkeys. The post-packing MDA concentration obtained in the control donkeys
was 39.62 μmol ± 4.16 μmol, and was not significantly different (P > 0.05) from the value of
35.97 μmol ± 2.88 μmol recorded in the experimental donkeys. In conclusion, the increase in
haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which
was ameliorated by AA administration.
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Keywords
Ascorbic acid, Donkey, Malondialdehyde, Eryhrocyte
Citation
13