Isolation and Antimicrobial Activity of β-Sitosterol-3-OGlucoside from Lannea Kerstingii Engl. & K. Krause (Anacardiacea)
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Date
2016
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Nitte University
Abstract
The emergence of more and more drug resistance bacteria has led to the study of the antimicrobial activity of the compound isolated
from Lannea kerstingii Engl. & K. Krause (Anacardiacea) since the active principles of many drugs found in plants are secondary
metabolites.Acompoundwas isolated using dry vacuumliquid chromatography and elutingwith CHCl -EtOAc andmonitored using TLC. 3
1 13 The glycoside was characterized using H NMR and C NMR spectra recorded in DMSO-d6 at 400 MHz and 125 MHz, respectively. The
antimicrobial activity of the compoundwas determined using agar diffusionmethod. Theminimuminhibitory concentration (MIC) and
minimum bactericidal/minimum fungicidal concentration (MBC/MFC) was determined using broth dilution method. The compound
isolatedwas found to be β-sitosterol-3-O-glucoside. The β-sitosterol-3-O-glucoside (200μg/ml)was active against S. aureus, Methicillin
Resistant Staphylococcus aureus, P. mirabilis, S. typhi, K. pneumoniae, E. coli, B. subtilis with zone of inhibition ranging from 24mm to
34mmand inactive against P. aeroginosa and Proteus vulgaris. Itwas also active against the fungi C. albicans and C. tropicalis but inactive
against C. krusei. The MIC ranged from25 to 50 μg/ml while the MBC/MFC ranged from50 to 200 μg/ml. These results show the wide
spectrumantimicrobial activity of β-sitosterol-3-O-glucoside.
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Keywords
sitosterol, glycoside, Lannea kerstingii, isolation