Browsing by Author "Yussuf, A. O."

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    Exposure to varied cage-size habitats alters pain sensitivity and inflammation-related biomarkers.
    (Elsevier, 2020) Oyewole, A. L.; Oyafemi, K. O.; Badmus, K. S. J. O.; Omoleye, K. S.; Abubakar, M. F.; Adeniyi-Raheem, O.; Amedu, A.; Lawal, D. L.; Ijiyode, A. O.; Yussuf, A. O.; Ishola, S. S.; Sulaimon, F. A.; Alli-Oluwafuyi, A.O.; Nafiu, A.B.; Akinola, O.; Olajide, O.J.; Amin, A.; Abdulmajeed, W.I.; Michael, O.S.; Adeyanju, O.A.; Ogunjimi, G.L.
    Background: Nature and size of rodent cages vary from one laboratory or country to another. Little is however known about the physiological implications of exposure to diverse cage sizes in animal-based experiments. Method: Here, two groups of male Swiss mice (Control group – Cage stationed, and Test group – Cage migrated) were used for this study. The cage-migrated mice were exposed daily to various cage sizes used across labora tories in Nigeria while the cage-stationed mice exposed daily to different but the same cage size and shape. At the end of the 30 days exposure, top-rated paradigms were used to profile changes in physiological behaviours, and this was followed by evaluation of histological and biochemical metrics. Results: The study showed a significant (p < 0.05) decrease in blood glucose levels (at 60 and 120 min of oral glucose tolerance test) in the cage-migrated mice compared to cage-stationed mice. Strikingly, peripheral oxi dative stress (plasma malondialdehyde) and pain sensitivity (formalin test, hot-and-cold plate test, and von Frey test) decreased significantly in cage-migrated mice compared to cage-stationed animals. Also, the pro-in flammation mediators (IL-6 and NF-κB) increased significantly in cage-migrated mice compared to cage-sta tioned mice. However, emotion-linked behaviours, neurotransmitters (serotonin, noradrenaline and GABA), brain and plasma electrolytes were not significantly difference in cage-migrated animals compared to cage stationed mice. Conclusion: Taken together, these results suggest that varied size cage-to-cage exposure of experimental mice could affect targeted behavioural and biomolecular parameters of pain and inflammation, thus diminishing research reproducibility, precipitating false negative/positive results and leading to poor translational outcomes.