Browsing by Author "Sunmonu, T.O."
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Item The Effect of Two African Mineral Dyes on the Activity of Alkaline Phosphatase in the Skin and Serum of Albino Rats(2006-09) Adebayo, G.B.; Sunmonu, T.O.; Adekola, F.A.; Olatunji, G.A.The effect of two African mineral dyes Yombo tumtum (YT) and Yombo fita (YF) on the activity of alkaline phosphatase (ALP) in the skin and serum of albino rats was investigated. The chemical analyses of the dyes were first carried out using solubility test, pH determination and X-ray fluorescence (XRF) elemental analysis. Six different concentrations (0.05, 0.15, 0.25, 0.5, 0.75 and 1.00%) of each dye were prepared using hydrogen peroxide as solvent. A total of 70 albino rats (Rattus norvegicus) were used for the study. The rats were grouped into 14 of 5 rats and were maintained on commercial feed for a period of 30 days, during which the experiment lasted. Group I served as the first control and the animals had distilled water applied on their heads. Group 2 which served as the second control contained rats on whose heads were applied the dissolving solvent i.e. hydrogen peroxide. Rats in group 3 to 8 had the various concentrations of YT (black) dye applied on their heads, while those in groups 9 to 14 had the various prepared concentrations of YF (white) dye applied on their heads. At the end of the experiment, blood samples were collected and portion of the head skin where the dyes were applied was cut. The activity of ALP was thereafter determined in the serum and skin of the experimental animals. The results revealed a significant decrease (p<0.05) in the activity of ALP as the concentration of the dye increased in both the serum and skin @JASEM.Item Effect of white African mineral hair dye on the activities of phosphatases and malondialdehyde level in selected tissues of albino rats(2005-09) Adebayo, G.B.; Sunmonu, T.O.; Adekola, F.A.; Olatunji, G.A.The effect of white African mineral dye Yombofita (YF) on the activities of alkaline phosphatase (ALP), acid phosphatase (ACP) and malondialdehyde (MDA) levels in the skin, liver, kidney and serum of albino rats was investigated. The chemical analysis of the dye was first carried out using solubility test, pH determination and X-ray fluorescence (XRF) elemental analysis. Six differentconcentrations (0.05, 0.15, 0.25, 0.5, 0.75 and 1.00%) of the dye were prepared using hydrogen peroxide (30 volume) as solvent. A total of 80 albino rats (Rattus norvegicus) were used for the study. The rats were divided into 8 groups of 10 each and were maintained on commercial feed for the period of the experiment i.e. 30 days. In group 1, the control group, the animals were applied distilled water on their heads, whereas in group 2 the vehicle i.e. hydrogen peroxide was applied. In groups 3 to 8 various concentrations of YF (white) dye ranging from 0.05, 0.15, 0.25, 0.5, 0.75 to 1.00% was applied respectively. At the end of the experiment, blood samples were collected and portions of the selected tissues were excised for the determination of ALP and ACP activities. The MDA level was also determined in the skin of experimental animals. The results revealed a significant decrease (p<0.05) with increasing concentration of the dye in the activities of ALP and ACP in the kidney and skin but not in the liver while the level of MDA in the skin increased significantly (p< 0.05) with increasing concentration of the dye.