Browsing by Author "Sunmonu, O. E."

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    Oral thymoquinone modulates cyclophosphamide‐induced testicular toxicity in adolescent Wistar rats
    (Wiley, 2022) Adana, M. Y.; Imam, A.; Bello, A. A.; Sunmonu, O. E.; Alege, E. P.; Onigbolabi, O. G.; Ajao, M. S.
    Cyclophosphamide (CYP) is an effective anti-cancer drug that is widely accepted, but it is not devoid of unintended toxic effects. Gonadal toxicity is reported as one of the side effects of its long-time use. This study examined the effects of thymoqui none (TQ) on the biological integrities of the testes after cyclophosphamide expo sure. Thirty adolescent male Wistar rats (100–110 g) were divided into six groups (n = 5), receiving normal saline (NS), 20 mg/kg of CYP (CYP), 5 mg/kg of TQ (TQ5), 10 mg/kg of TQ (TQ10), 20 mg/kg of CYP and 5 mg/kg of TQ (CTQ5), and 20 mg/ kg of CYP and 10 mg/kg of TQ (CTQ10) respectively. On the 22nd day, blood, semen and testicular samples were collected for the assay of serum reproductive hormones (follicle-stimulating (FSH) and luteinizing (LH) hormones), semen analysis and testicu lar histology and proliferating cell nuclear antigen (PCNA) expression. The results re vealed that CYP exposure affected functional and structural integrities of the testes, by depleting sperm count and motility, testosterone, LH, spermatogenic and mature sperm cell population, Leydig cells and PCNA immunoreactive proliferating cells. TQ interventions were able to reverse all cytotoxic CYP impacts, but with differential activities on the hormonal concentrations, specifically LH and FSH. Cumulatively, thymoquinone may be a potent agent against cyclophosphamide effects on the physi ological, regeneration and histological integrities of the testes, as observed in this study.
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    Protective Potential of Thymoquinone on Cyclophosphamide-Induced Hepatotoxicity in Rat
    (National Association of Specialist Medical Doctors in Academics in Nigeria, 2022) Adana M. Y.; Opabunmi, E. T.; Sunmonu, O. E.; Bello, A. A.; Onigbolabi, O. G.; Imam A.; Ajao, M. S.
    Background: Cyclophosphamide (CYC) has been known as an anticancer drug with several side effects on various organs such as the liver. In this study, the hepatoprotective properties of thymoquinone (TQ) were tested to decrease the damaging effects of Cyclophosphamide on the liver. Objective: To study and observe the possible ameliorative effect of thymoquinone on cyclophosphamide-induced hepatic toxicity in Wistar rats. Methods: Thirty Wistar male Adolescent (8 – 9 weeks old) rats weighing 70g – 150g were divided into Six groups; 5 rats per group (n=5) and treated orally for 21 consecutive days. Group A served as the control, and the rats in this group received normal saline. Group B was treated with 20mg/kg of cyclophosphamide. Group C was treated with 5mg/kg of Thymoquinone. Group D was treated with 10mg/kg of Thymoquinone. Group E was treated with 20mg/kg of cyclophosphamide and 5m/kg of Thymoquinone while Group F animals were treated with 20mg/kg of Cyclophosphamide and 10mg/kg of Thymoquinone. Results: The Cyclophosphamide treated group showed significant decreases in the body and liver weight, distorted hepatic cytoarchitecture, and reduction in hepatic function whereas co-treatment with TQ, showed protective effects on Cyclophosphamide-induced hepatotoxicity damage. Conclusion: These results suggest that the administration of Thymoquinone may have a protective potential on Cyclophosphamide induced hepatotoxicity.