Browsing by Author "Sarvendra Kumar"

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    Additional feeding of vitamin–mineral‐based nutraceutical to stress‐exposed rohu, Labeo rohita, enhances the IGF‐1 gene expression and growth
    (Hindawi Limited, 2020-04-03) Shamna Nazeemashahul; Narottam Prasad Sahu; Parimal Sardar; Femi John Fawole; Sarvendra Kumar
    One thousand and eighty rohu fingerlings with an average size of 8.5 ± 0.5 g were randomly distributed in 6 distinct experimental groups in triplicates, where the first three groups were exposed to high stocking density (HSD; 20 nos/ 75 L water) with restricted feeding (RF), viz. THR0 (HSD, RF, 0% nutraceutical), THR1 (HSD, RF, 0.1% nutraceutical) and THR5 (HSD, RF, 0.5% nutraceutical), and second three groups were exposed to normal stocking density (NSD; 10 nos /75 L water) with satiation feeding (SF) and designated as TNS0 (NSD, SF, 0% nutraceutical), TNS1 (NSD, SF, 0.1% nutraceutical) and TNS5 (NSD, SF, 0.5% nutraceutical). The weight gain (WG) %, specific growth rate (SGR), protein efficiency ratio (PER) and IGF-1 expression were significantly lower, whereas feed conversion ratio (FCR), serum superoxide dismutase (SOD) and catalase activities were significantly higher in fish exposed to HSD with RF at monthly sampling. Feeding of 0.1% dietary nutraceutical resulted in a significantly higher WG%, SGR, PER and IGF-1 expression and lower FCR, SOD and catalase activities at the end of the third and fourth month of feeding in HSD with RF groups. The present study concluded that 3-month feeding of 0.1% nutraceutical could improve growth and reduce stress in fish.
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    Changes in weight gain, digestive and metabolic enzyme activities in Labeo rohita fingerlings in response to multiple stress exposure and dietary nutraceutical
    (Springer Science and Business Media LLC, 2021-10) Nazeemashahul Shamna; Narottam Prasad Sahu; Parimal Sardar; Fawole Femi John; Sarvendra Kumar
    A feeding trial was conducted for 4 months to study the effect of a nutraceutical conglomerate at different levels (0, 0.1, and 0.5%) on stress-exposed (high stocking density, H and feed restriction, R) Labeo rohita fingerlings. Six isonitrogenous diets with a crude protein of 35% were prepared for the different treatments, viz. THR0, THR1, THR5, TNS0, TNS1 and TNS5. Stress significantly reduced the weight gain and feeding of 0.1% nutraceutical improved it in both stress-exposed and non-exposed groups from 3 months onwards. Two-and three-month stress-exposed groups exhibited lower (p < 0.05) protease activity, while amylase activity was significantly higher in 2-month stress-exposed groups. Higher lipase activity was found in stress-exposed groups irrespective of the duration of stress. Higher serum glutathione peroxidase (GPx), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and hepatic and muscle lactate dehydrogenase (LDH) activities were observed in the THR0 groups; however, THR1 showed a lower activity of these enzymes. Increased malate dehydrogenase (MDH) and glucose-6-phosphate dehydrogenase (G6PDH) activity was recorded in the 1st and 2nd months stress-exposed groups. However, THR1 group exhibited a reduced MDH and G6PDH activity compared to THR0 group in 1st and 2nd months. Hence, it can be concluded that the duration of multiple stress exposure adversely affected the digestive and metabolic enzymes activities and feeding of 0.1% nutraceutical conglomerates could restore the activities of digestive and metabolic enzymes in rohu fingerlings.
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    Effect of dietaryHouttuynia cordataleaf meal and leaf extract on the growth performance, nutrient utilization and expression of IGF‐I gene inLabeo rohita
    (Hindawi Limited, 2019-02-11) Chetan K. Garg; Narottam P. Sahu; N. Shamna; Ashutosh D. Deo; Femi J. Fawole; Sarvendra Kumar; Manas K. Maiti
    A 60‐day feeding trial was conducted to evaluate the effects of feeding Houttuynia cordata leaf meal and extract on the growth performance, nutrient utilization and expression of insulin‐like growth factor‐I (IGF‐I) in Labeo rohita fingerlings. Two hundred seventy fingerlings were randomly distributed in six experimental groups in triplicates. Six isonitrogenous (350 g/kg CP) and isocaloric (17 MJ/kg DE) diets were prepared with graded levels of leaf meal (LM) and leaf extract (LE), namely C (control, without LE and LM), LE‐0.25 (2.5 g/kg LE), LE‐0.5 (5 g/kg LE), LE‐1.0 (10 g/kg LE), LM‐1.0 (10 g/kg LM) and LM‐2.0 (20 g/kg LM). Significantly (p < 0.05) higher IGF‐I expression, weight gain %, specific growth rate and lower feed conversion ratio were observed in LM‐1.0 followed by LE‐1.0 group compared to control group. Higher protease activity was observed in LE‐1.0 compared to other experimental groups, while no significant changes were found for amylase and lipase activities. Compared to control, muscle alanine aminotransferase and aspartate aminotransferase activities were significantly higher in treatment groups except for LM‐2.0. These results suggest that either LE‐1.0 or LM‐1.0 can be supplemented in the diet to improve the growth of L. rohita. However, the supplementation of 10 g H. cordata leaf meal/kg feed can be the better option considering the cost of extraction.