Browsing by Author "Sabiu, Saheed"

Now showing 1 - 5 of 5
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    An appraisal of the metabolites, pharmacological and biotechnological significance of edible mushrooms
    (Taylor and Francis and University of South Africa Press for Royal Society of South Afric, 2021) Bolaniran, Tolulope; Jamiu, Abdullahi T.; Garuba, Taofeeq; Wudil, Alhassan M.; Adeola, Henry A.; Sabiu, Saheed
    Mushrooms are universally valued for their dietary and therapeutic significance. Their importance could be attributed to numerous nutritive and dietary substances including polysaccharides, terpenoids, phenolics and other light molecular bioactive compounds. These compounds offer optimal health benefits and have been identified as a potential source of nutritional and medicinal products against several debilitating and food-related disorders. Here, we present an updated synopsis of the medicinal attributes of mushrooms, while also highlighting the technological advancements in their cultivation that have led to the birth of engineered species with improved traits that could alleviate malnourishment and contribute towards food security, offer health benefits, and provide efficient ways of waste management.
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    Haematological, Serum Biochemical and Histopathological Changes in Acute and Sub-Chronic Aqueous Extract of Oyster Mushroom in Male Wistar Rats
    (Faculty of Sciences, Rajshahi University, Bangladesh., 2023) Garuba, Taofeeq; Olabanji, Islamiyat T.; Awogboro, Mariam O.; Olahan, Ganiyu S.; Atunwa, Soliu A.; Ahmed, Akeem O.; Aremu, Abdulfatai; Bolaji Mashhod; Adegboye, A. A.; Sabiu, Saheed
    This study aimed at evaluating toxicological implications of aqueous P. ostreatus extract (POE) in male Wistar rats. POE was prepared in 1:10 (pulverized P. ostreatus : distilled water). In acute toxicity test, single oral dose of 2 mL/kg of POE was administered and observed for 28 days. The sub-chronic toxicity study was conducted by daily oral administration of graded doses (0.25, 0.50 and 0.75 mL/kg b.w) of the extract for 28 days. Clinical signs of toxicity, hematological, serum biochemical parameters and histopathological studies were subsequently evaluated. No treatment-related signs of toxicity or mortality in the animals were recorded in both toxicity tests. Rats administered with lowest dose of POE (25 mL/kg) had highest percentage weight gain. POE had no significant difference (P>0.05) on Red Blood Cell, White Blood Cell (WBC) and differential WBC, and serum biochemistry across all the treated groups when compared to the controls. The result of photomicrographs of stomach, spleen, heart, lung, kidney and liver showed a well outlined arrays of normal tissues in both acute and sub-chronic doses connoting that POE had no toxic effect on them. In view of these, POE may be concluded to be non-toxic within the tested doses and period of investigation
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    Membrane stabilization and probable mechanisms of hypoglycemic activity of fruit extract of Solanum incanum L. (Solanaceae)
    (Springer, 2018) Sabiu, Saheed; Ajani, E. O.; Aladodo. R. A.; Garuba, Taofeeq; Agunbiade, M. O.; Alimi, A. A.; Lekena, N.; Abdulrasaq, M.
    This study investigated the antioxidant, membrane stabilization, and mechanisms of hypoglycemic attribute of aqueous fruit extract of Solanum incanum L. in vitro. The extract was separately incubated with α-amylase and α-glucosidase and subsequently with starch and p-nitrophenylglucopyranoside, respectively, while its glucose uptake regulatory effect was evaluated against Saccharomyces cerevisiae. The data obtained showed that the extract significantly modulated the rate of glucose uptake by yeast cells and had respective competitive and uncompetitive inhibitory influence on α-glucosidase and α-amylase with overall half-maximal values of 2.98 and 7.08 mg mL−1 relative to that of acarbose (4.93 and 5.59 mg mL−1). S. incanum also markedly halts free radicals in a manner comparable to silymarin. With a display of 86.67% potency against heat-induced hemolysis of bovine serum erythrocytes, the extract could also be said to have shown marked membrane stabilization activity. The effects shown by S. incanum at the investigated concentrations may be ascribed to its analytes as revealed by the results of the chromatographic analysis. Put together, besides being antioxidative, regulation of glucose uptake rate and enzymes’ activities are its probable mechanisms of hypoglycemic potential and has further provided baseline evidence for its antidiabetic application.
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    Metabolites profiling and molecular docking identification of putative leads from endophytic Phyllosticta capitalensis as modulators of key druggable structural targets of rotavirus A
    (Taylor and Francis and University of South Africa Press for Royal Society of South Africa., 2023) Garuba, Taofeeq; Govender, Rayhaan; Abdulsalam, Habibah; Sabiu, Saheed
    Rotavirus-induced gastroenteritis is a global health concern and the leading cause of death in low-income countries and children under the age of five. While rotavirus A (RVA) vaccines are available, there accessibility and efficacy remain challenging, especially in the third world countries. This has necessitated the need to develop therapeutics to manage RVA infection. This study investigated gas chromatography-mass spectrometry-derived Phyllosticta capitalensis metabolites as potential therapeutics against the key structural proteins [VP8* portion of the VP4 (spike protein), the VP7 (capsid protein), and the VP1 (RNA-dependent RNA polymerase)] of RVA using molecular docking and ADME approaches. The results revealed that four [lup-20(29)-en-one, A’-neogammacer-22(29) ene, stigmasta-4,7,22-trien-3-α-ol, and fucosterol] of the 67 metabolites had favourable affinity for the three structural proteins and belonged to classes of metabolite of antiviral importance. Further analysis and pharmacokinetic profiling showed that the pentacyclic triterpenoids, lup-20(29)-en-one and A’- neogammacer-22(29)-ene, had the potential to be developed as antivirals against RVA infection and effort is underway in this direction.
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    TOXICOLOGICAL IMPLICATIONS OF ACUTE AND REPEATED DOSE ADMINISTRATIONS OF HYDROETHANOLIC EXTRACT OF CITRULLUS VULGARIS SEEDS IN WISTAR RATS
    (Department of Biology, West University of Timişoara, Romania, 2017) Garuba, Taofeeq; Sabiu, Saheed; Ambali, Ismail A.; Feyijin, Iyanuoluwa B.
    In spite of the wide therapeutic usage of formulations of Citrullus vulgaris seeds, there is still paucity of enriched biochemical information on its safety profiles in animal. Hence, the present study evaluated the acute and sub-chronic toxicological implications of its hydroethanolic extract in Wistar rats using OECD guidelines. In the acute toxicity test, single oral administration of 5000 mg/kg body weight (b.w.) of the extract was given to the animals and observed for 14 days. The sub-chronic toxicity study was conducted by daily oral administration of graded doses (100, 500 and 1000 mg/kg b.w.) of the extract for 28 days. Clinical signs of toxicity, behavioral changes, hematological, and biochemical parameters were subsequently evaluated. The extract at 5000 mg/kg b.w. produced no treatment-related signs of toxicity, behavioral changes or mortality in the animals. Thus, its No Observed Adverse Effect Level dose was estimated to be greater than 5000 mg/kg b.w. In the sub-chronic study, except for the significantly altered serum activity of aspartate aminotransferase at 100 and 500 mg/ kg b.w., treatments with the extract revealed no significant difference in hematological and clinical biochemistry indices relative to the control. However, the extract dose-dependently and significantly increased the body weight of the treated animals compared to the control group. Cage side observations also recorded no treatment-induce signs of toxicity. In view of these, hydroethanolic seeds extract of C. vulgaris may be concluded to be non-toxic within the tested doses and period of investigation. Hence, it could be adjudged relative safe for consumption in rats.