Browsing by Author "Oyewopo, AO"
Now showing 1 - 5 of 5
Results Per Page
Sort Options
Item Central cholinergic markers and spartial memory performance in rats following administration of Rauwolfia vomitoria and chlorphromazine(Library and Publications Committee University of Ilorin, Ilorin, Nigeria, 2015) Ajao, MS; Imam, A; Adana, MY; kareem, SB; Alabi, AS; Olawepo, A; Okesina, AA; Kadri, RE; Oyewopo, AOItem Cytotoxic potentials of thiocyanate administration on the liver of male Wistar rats (Rattus norvegicus)(Anatomy Journal of Africa,, 2016) Alabi, AS; Nurudeen, MN; Omotoso, Gabriel Olaiya; Oyewopo, AO; Olawepo, A; Ajao, MSThe use of thiocyanate as an anti-sickling drug is currently on the increase among sickle cell disease (SCD) patients. The continuous use of this substance without sufficient toxicity data does not guaranty continuously functional and healthy internal organs among the SCD patients that are susceptible to multi-organ failure such as hepatic failure. Hence this study was performed to elucidate the consequence(s)of thiocyanate administration on the liver of adult male wistar rats. Twenty adult male wistar rats with an average weight of 234.5g were used. The rats were grouped into four (A, B, C & D) with five animals in each group. Group A represented the control and was given only 1ml of distilled water daily while B,C,&D received 1ml of thiocyanate solution at doses of 10mg/Kg/day, 20mg/Kg/day, & 30mg/Kg/day for twenty-eight (28) days. The administration was carefully done with the use of an oral cannula. Thereafter, the rats were sacrificed via cervical dislocation. The rats were dissected and blood samples were immediately collected from the apex of the heart for the analysis of serum total bilirubin. A fraction of the liver was cut to prepare homogenates for biochemical enzymes (alanine aminotransferase-ALT, aspartate aminotransferase-AST) analysis. Thereafter the animals were wholly perfused with normal saline and then fixed with 4% paraformaldehyde. The fixed liver tissues were then taken for histological assessments. The slide sections (H&E and PAS stains) in the treated groups showed varying degrees (mild to severe disruption of hepatocellular morphology plus prominence and widening of sinusoids) of thiocyanate-induced liver damage. But hepatocellular appearance was normal in the control group. Biochemical assays of serum total bilirubin and tissue AST increased fairly with increasing dose although this was not significant. The increase in tissue level of ALT in group B was also not significant with respect to the control. But increase in ALT level was found to be significant in groups C&D when compared with the control. Therefore, this study can be used to infer that SCN use in sickle cell disease management regimen can cause hepatocellular damage in wistar rats.Item Garlic consumption alters testicular histology and anti-oxidant status in Wistar rats.(The Tropical Journal of Health Sciences, 2012) Omotoso, Gabriel Olaiya; Oyewopo, AO; Onanuga, Ismail Olashile; Enaibe, BUGarlic is known for its cell and tissue-protective functions. This study examined the effects of aqueous garlic extract on the histology of the testes of Wistar rats and erythrocyte superoxide dismutase (SOD) activity. Twenty-four (24) rats with an average weight of 116 g were used. They were randomly grouped into three: Group A Control: was given distilled water; Group B: was given 500 mg/kg/d aqueous garlic extract; and Group C: received 1000 mg/kg/d aqueous garlic extract; all for 28 days. Findings revealed progressive depletion in sperm cell population, reduction in size of seminiferous tubules, and widening interstitial space. SOD activity was significantly reduced in both treated groups in a dose-dependent fashion. These histological observations and the depletion in endogenous anti-oxidant status associated with the administration of garlic could result in significant affectation of male reproductive functions.Item Lawsonia inermis leaf extract mitigates aluminium-induced testicular toxicity in wistar rats: Immunohistochemical study(Centre for Malaria Research & Phytomedicine, University of Port-Harcourt, 2018) Olawuyi, TS; Oladipo, GS; Alabi, AS; Oyewopo, AO; Omotoso, GOItem Liver enzymes derangement and the influence of diet in animals given oral albendazole(Nigerian Medical Journal, 2013) Omotoso, Gabriel Olaiya; Enaibe, BU; Oyewopo, AO; Onanuga, Ismail OlashileBackground: Albendazole is used as an anthelmintic in the treatment of some parasitic infections. This study determined how the effects of albendazole on liver enzymes are influenced by diet. Materials and Method: Thirty adult male Wistar rats of mean weight 304.12 ± 11.34 g were randomly grouped into five: Group A: Control, was given rat pellets and water only; Group B received 15 mg/kg/d of albendazole while fasting; Group C received 15 mg/kg/d of albendazole with fatty meal; Group D received 15 mg/kg/d of albendazole with normal diet (rat pellets); and, Group E received 30 mg/kg/d of albendazole with normal diet (rat pellets); they were given orally for 3 consecutive days. The animals were sacrificed thereafter and blood samples obtained for quantitative study of the serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Results: Significant elevation in the serum levels of the transaminases especially in animals which were on their normal diet (rat pellets), while ALP was either reduced or increased based on dietary factors. Conclusions: Oral administration of albendazole before meal or with a fatty diet could help limit severe elevation of liver enzymes associated with its use, while still ensuring optimal efficacy.