Browsing by Author "Oyewopo A.O.,"

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    CRYPTORCHIDISM AND HYPERTHERMIA-INDUCED TESTICULAR INJURY IN ADULT MALE RATS; INTERVENTION OF ALPHA TOCOPHEROL
    (Society for Experimental Biology of Nigeria, 2023) Adunmo G.O.,; Lawal A.Z.,; Oyewopo A.O.,; Oyewopo C.I.,; Stephen D.A.,; Akindehin O.A.,; Adunfe O.O.,; Adeleke O.S.,; Hamzat F.O.,; Ashamu E.A.,
    The objective of this study is to determine the effects of cryptorchidism and hyperthermia on the testicular cytoarchitecture of adult male rats, quality and quantity of semen and the level of oxidative stress in the testes before and after intervention of Alpha-tocopherol (Vitamin E). Twenty-four (24) adult Wistar rats were used for this experiment and they were divided into seven groups (n=4). Experimental cryptorchidism was carried out in some animals while others were subjected to hyperthermia from two sources (hot oven and light bulb) for 28 days; followed by intervention of 100mg/kg alpha-tocopherol for fourteen (14) days. All animals were then euthanized under anaesthesia. The testes were excised and fixed and processed using haematoxylin and eosin technique. Plasma was assayed for glutathione, superoxide dismutase (SOD) and malondialdehyde (MDA). Cryptorchidism and hyperthermia caused in cycto-architectural distortion in the testicular structures of exposed animals and significant reductions in caudal epididymal sperm count and motility, morphology and life and death ratio. Plasma SOD and GLU levels also reduced significantly in exposed animals while MDA levels increased significantly, indicating peroxidation of testicular tissue. Alpha-tocopherol due to its anti-oxidative properties impeded lipoperoxidation of the membrane, conserving cell union and increasing the amount of germ cells in the seminiferous epithelium. At doses and durations tested, alpha-tocopherol (αT) reverted to a significant extent the effects of cryptorchidism and hyperthermia by reducing apoptosis in germ cells, promoting cell survival and reducing histological alterations to the seminiferous epithelium.
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    THERAPEUTIC EFFECT OF QUERCETIN ON THE HYPOTHALAMUS OF STZ-NICOTINAMIDE INDUCED DIABETIC RATS
    (Society for Experimental Biology of Nigeria, 2023) Lawal A.Z.,; Adunmo G.O.,; Oyewopo A.O.,; Oyewopo C.I.,; Stephen D.A.,; Akindehin O.A.,; Adunfe O.O.,; Adeleke O.S.,; Hamzat F.O.,; Ashamu E.A.
    Diabetes mellitus (DM) is a chronic metabolic disorder characterized by persistent hyperglycemia and disturbances in sugar, fat, and protein metabolism. The aim of this study was to investigate the therapeutic effect of quercetin on the hypothalamus in adult male Wistar rats with STZ-nicotinamide-induced diabetes. Forty rats were divided into eight groups, each receiving different treatments. The control group received a standard diet, while other groups were administered various doses of quercetin alone, diabetes induction agents alone, or a combination of quercetin and diabetes induction agents. Treatments were administered orally for eight weeks. Results revealed significant differences in body weight among the groups, with diabetic rats showing a decrease compared to the control group. However, rats treated with quercetin or metformin exhibited a significant increase in body weight compared to the diabetic control group. Additionally, diabetes induction significantly decreased plasma insulin, plasma testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone levels, while quercetin treatment led to significant increases in these hormone levels. Histological examination of the hypothalamus showed notable findings. Control group rats displayed normal histomorphological features, while diabetic control rats exhibited swollen and distorted hypothalamic cells with cellular apoptosis. Interestingly, rats treated with quercetin showed a reduction in apoptotic cells, indicating a protective effect on hypothalamic cells. In conclusion, our findings suggest that quercetin possesses potential therapeutic properties in ameliorating the detrimental effects of STZ-nicotinamide-induced diabetes on the hypothalamus in adult male Wistar rats.