Browsing by Author "Oyewopo A.O."

Now showing 1 - 7 of 7
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    Central Cholinergic Markers and Spatial memory Performance in rats following administration of Rauwolfia Vomitoria and Chlorpromazine
    (Centre Point Journal (Science Edition). Published by Library and Publication Committee, University of Ilorin, 2015) Ajao M.S.; Imam A.; Adana M.Y.; Kareem S.B.; Alabi A.S.; Olawepo A.; Okesina A.; Kadir R.E.; Oyewopo A.O.
    The study was designed to compare the effects of Rauwolfia vomitoria and chlorpromazine on metabolic activities, spatial memory performance, central cholinergic markers, oxidative stress markers and neurohistoarchitecture in the brain of adult wistar rats. Twenty five adult male wister rats weighing between 200 - 230 g were divided into five groups (A-E) of five rats each. Group A (control) received 2mls of normal saline daily, Group B received 5mg/kg of chlorpromazine, Group C received 10 mg/kg of chlorpromazine, Group D received 150 mg/kg of R. vomitoria and Group E received 300 mg/kg of R. vomitoria orally. All the medications were given daily for 21 days. Body weights were taken weekly and recorded. A y-maze apparatus was used to assess the spatial memory performance in the rats at days 14 and 21 of the experiment. All the animals were euthanized using 20mg/kgbw of intramuscular ketamine, cardially perfused with 4% paraformaldehyde and the brains were removed, some were homogenised for analysis of central cholinergic markers and others for histological analysis. Low doses of chlorpromazine increase body weight and Rauwolfia vomitoria increase central cholinergic markers. The findings of the study show that R.vomitoria and chlorpromazine exert differential and dosage dependent effects on body weight, central cholinergic neurons and learning performance. The conclusion from the study indicates that R. vomitoria could be a better alternative drug in the treatment of psychosis with lesser metabolic and central nervous system side effects.
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    Central cholinergic markers and spatial performance in rats following administration of rauwolfia vomitoria and chlorpromazine
    (Center Point Journal (Science Edition). The Official Journal of the Library and Publications Committee, University of Ilorin, Ilorin., 2015) Ajao M.S.; Imam A.; Adana M.Y.; Kareem S.B.; Alabi A.S.; Olawepo A.; Okesina A.A.; Kadir R.E.; Oyewopo A.O.
    The study was designed to compare the effects of Rauwolfia vomitoria and chlorpromazine on metabolic activities, spatial memory performance, central cholinergic markers, oxidative stress markers and neurohistoarchitecture in the brain of adult wistar rats. Twenty five adult male wister rats weighing between 200 - 230 g were divided into five groups (A-E) of five rats each. Group A (control) received 2mls of normal saline daily, Group B received 5mg/kg of chlorpromazine, Group C received 10 mg/kg of chlorpromazine, Group D received 150 mg/kg of R. vomitoria and Group E received 300 mg/kg of R. vomitoria orally. All the medications were given daily for 21 days. Body weights were taken weekly and recorded. A y-maze apparatus was used to assess the spatial memory performance in the rats at days 14 and 21 of the experiment. All the animals were euthanized using 20mg/kgbw of intramuscular ketamine, cardially perfused with 4% paraformaldehyde and the brains were removed, some were homogenised for analysis of central cholinergic markers and others for histological analysis. Low doses of chlorpromazine increase body weight and Rauwolfia vomitoria increase central cholinergic markers. The findings of the study show that R.vomitoria and chlorpromazine exert differential and dosage dependent effects on body weight, central cholinergic neurons and learning performance. The conclusion from the study indicates that R. vomitoria could be a better alternative drug in the treatment of psychosis with lesser metabolic and central nervous system side effects.
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    EFFECTS OF PHOSPHODIESTERASE 5 INHIBITOR (VIAGRA) ON BIOCHEMICAL PARAMETERS OF L NAME-INDUCED TESTICULAR TOXICITY IN ADULT MALE WISTAR RATS
    (KJHS, 2024) Adunmo G.O.; Oyewopo A.O.; Akindehin O.A.; Stephen D.A; Ogunbiyi O.E.; Abdulazeez I.A.; Lawal A.Z.; Adeleke O.S; Akingbade A.M.; Opoola F.O.; Ajayi S.O.; Ajayi A.J.
    ABSTRACT Testicular toxicity is a growing concern in today's world, with various factors contributing to its prevalence. Nitric oxide (NO) imbalance, often induced by N (gamma)-nitro-L-arginine methyl ester (L-NAME), is a significant factor associated with testicular dysfunction. Sildenafil (Viagra), a phosphodiesterase type 5 inhibitor, has shown promise in improving testicular function by modulating NO levels. This study aimed to investigate the role of Sildenafil (Viagra) on biochemical parameters of L-NAME induced testicular toxicity in Wistar rats. Twenty-four adult male Wistar rats were divided into four groups: Control (physiological saline-treated), L-Name (L-Name-induced testicular toxicity), PDE (Sildenafil-treated), and L-Name + Sildenafil (co-treatment) and subjected to a 56-day treatment regimen. At the end of the administration, the animals were sacrificed, tissues collected and biochemical and histological assessments were performed. Findings revealed that L-Name administration led to a significant decreased in nitric oxide levels, follicle stimulating hormone, luteinizing hormone, testosterone and increase in oxidative stress when compared to the control group. Furthermore, histological analysis demonstrated structural alterations in the testes of L NAME-treated rats, indicative of testicular toxicity. Rats treated with Sildenafil showed a slight reversal of these adverse effects. Also, slight reversals of impaired spermatogenesis were evident in the co-treatment group. This study provides compelling evidence for the potential therapeutic role of sildenafil in ameliorating L-NAME-induced testicular toxicity in adult male Wistar rats. Keywords: Testicular toxicity, spermatogenesis, oxidative stress, testosterone.
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    Low dose bitter leaf improves sperm quality and boosts sperm counts disrupted by the immunosuppressive effects of prednisolone
    (Shahid Sadoughi University of Medical Sciences, 2020) Kadir R.E.; Ibrahim A.; Ibrahim B.A.; Gwadabe S.M.; Sulaiman-Jaji R.; Adigun M.F.; Oyewopo A.O.
    Background: Synthetic prednisolone (PRED) is a widely used over-the-counter glucocorticoid. Glucocorticoids have inhibitory effects on the immune system and are often used as immunosuppressive agents. Suppressed immunity may impact fertility via the hypothalamic-pituitary-adrenal axis. Bitter leaf (BL) has been shown to improve sperm parameters, but its effects on immunosuppression-associated infertility have not yet been documented. Objective: To determine the fertility effects of bitter leaf on immunosuppressed Wistar rats. Materials and Methods: A total of 30 male adult Wistar rats were randomly assigned to 6 groups (n = 5/each). Group A served as a control and were given distilled water in addition to normal feeds, group B received 2 mg/kg PRED for 14 days and served as the standard immunosuppressed group, and groups C-F were immunosuppressed as in B but in addition received 50 mg/kg levamisole, low-dose (250 mg/kg) BL, highdose (375 mg/kg) BL, and low-dose BL + levamisole, respectively. The CD4 counts, hematological parameters, and sperm parameters were analyzed and compared. Results: There were significant decreases in sperm motility, progressive motility, morphology, and life/death ratio in the animals given PRED only compared to the controls (p = 0.002, 0.001, 0.001, and 0.01, respectively). These were significantly increased in the treated groups, and animals given levamisole and 250 mg/kg BL showed significantly increased sperm counts compared to the controls (p = 0.04 and p = 0.04, respectively). Conclusion: Low-dose BL (250 mg/kg) restored the sperm parameters altered by prednisolone administration.
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    Moringa oleifera and Musa sapientum ameliorated 7,12-Dimethylbenz[a]anthracene-induced upregulations of Ki67 and multidrug resistance 1 genes in rats
    (Qassim University, 2021) Akinlolu A.A.; Oyewopo A.O.; Kadir R.E.; Lawal A.; Ademiloye J.; Jubril A.; Ameen M.O.; Ebito G.E.
    Objectives: Moringa oleifera (MO) and Musa sapientum (MS) are plants of ethnomedicinal importance. We evaluated the effects of MOF6 (extracted from MO leaves) and MSF1 (extracted from MS suckers) on immunomodulations of Ki67 (proliferation biomarker) and multidrug resistance 1 (MDR1) genes in the liver of rats in 7,12-Dimethylbenz[a]anthracene (DMBA)-induced hepatotoxicity and mutagenesis to determine their antiproliferation, anti-drug resistance, and anticancer potentials. Methods: Forty-five adult male rats were randomly divided into nine groups (n = 5). Groups 1 and 2 received physiological saline and 15 mg/kg bodyweight of DMBA, respectively. Groups 3 and 4 received 15 mg/kg bodyweight DMBA and were treated with 15 and 30 mg/kg bodyweight of MOF6, respectively. Group 5 received 15 mg/kg bodyweight DMBA and was treated with 10 mg/kg bodyweight of MSF1. Group 6 received 15 mg/kg bodyweight DMBA and was treated with 3.35 mg/kg bodyweight of doxorubicin and intravenous injection of 0.5 ml/200 g of cisplatin. Groups 7–9 received only 15 and 30 mg/kg bodyweight of MOF6 and 10 mg/kg bodyweight of MSF1, respectively. DMBA, doxorubicin, and extracts doses were administered orally. The duration of our experimental procedure was 8 weeks. Consequently, liver histopathology (hematoxylin and eosin technique) and enzyme-linked immunosorbent assay homogenates’ concentrations of Ki67 and MDR1 were evaluated. Computed data were statistically analyzed (P ≤ 0.05). Results: Results showed normal histoarchitectures of the liver in all groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased concentrations (P ≥ 0.05) of Ki67 and MDR1 in Groups 3–9 compared with Group 2. Therefore, MOF6 and MSF1 ameliorated DMBA-induced hepatotoxicity, abnormal proliferation, and drug resistance. Conclusion: MOF6 and MSF1 possess antiproliferation, anti-drug resistance, and anticancer potentials.
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    Reproductive System and Perineum
    (Department of Anatomy, University of Ilorin., 2024) Kadir E.R.; Oyewopo A.O.; Alabi A.S.
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    Urinary System
    (Department of Anatomy, University of Ilorin., 2024) Imam A.L.; Kareem S.B.; Kadir E.R.; Oyewopo A.O.