Browsing by Author "Oyewopo, Adeoye"

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    Anti-Toxic Principles from Morinda lucida And Annona muricata Down-Regulated Ki67 and Multi-Drug Resistance1 Genes In Lead-Induced Hepato-Toxicity In Rats
    (Penerbit Universiti Sains Malaysia, 2022-11-16) Akinlolu, Adelaja A.; Omohimoria, Temitope; Oyewopo, Adeoye; Kadir, Risikat E.; Ameen, Mubarak O.
    Morinda lucida (ML) and Annona muricata (AM) are ethno-medicinal plants with antioxidant potentials. In addition, lead is a toxic pollutant of global health concerns. This study evaluated the effects of column chromatography-extracted ethanolic fractions of ML and AM leaves on immuno-modulations of Ki67 and multi-drug resistance1 (MDR1) proteins in the liver of rats in lead acetate (LA)-induced hepato-toxicity in-order to determine their hepato-protective, anti-proliferation, anti-drug resistance and anti-cancer potentials. Sixty adult female rats were randomly divided into 12 groups (n = 5). Groups 1 and 2 received physiological saline and 100 mg/kg bodyweight of LA, respectively, for 5 weeks. Groups 3–6 received 100 mg/kg bodyweight LA for 2 weeks followed by post-treatments with 7.5 mg/kg and 15 mg/kg bodyweight of ML, and 7.5 mg/kg and 10 mg/kg bodyweight of AM, respectively, for another 3 weeks. Groups 7–10 received 7.5 mg/kg and 15 mg/kg bodyweight of ML, and 7.5 mg/kg and10 mg/kg bodyweight of AM, respectively, for 5 weeks. Groups 11 and 12 received co-administrations of 100 mg/kg bodyweight LA simultaneously with 15 mg/kg bodyweight ML and 10 mg/kg bodyweight of AM, respectively, for 5 weeks. Drugs and extracts were administered orally. Consequently, liver histopathology (heamatoxylin and eosin technique) and enzyme linked immunosorbent assay (ELISA) homogenates’ concentrations of Ki67 and MDR1 were evaluated. Computed data were statistically analysed (p ≤ 0.05). Results showed normal liver histology in all groups. Post-treatments of LA-induced hepato-toxicity resulted in statistically significant (p ≤ 0.05) and non-significant decreased concentrations (p ≥ 0.05) of Ki67 and MDR1 in Groups 3–12 compared with Group 2. These observations indicated that ML and AM ameliorated LA-induced hepato-toxicity, abnormal proliferation, drug resistance and mutagenesis.
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    Morinda lucida and Annona muricata reduced hepatic lipid peroxidation and promoted melatonin/TNFα/p53-mediated apoptosis in sodium arsenite-induced toxicity in rats
    (The Spanish Association of Anatomy (SAE) and Mexican Society of Anatomy, 2022-07-28) Akinlolu, Adelaja; Oyewopo, Adeoye; Kadir, Risikat; Ameen, Mubarak; Owoniyi, Victor; Adam, Fauzeeyah; Okeleye, Shukrat
    Arsenic-induced carcinogenesis can result in cancers of the liver in exposed organisms. This study evaluated anticancer potentials of MLF1 and AMF1 extracted from Morinda lucida and Annona muicata leaves respectively in Sodium arsenite (SA)-induced toxicity in rats. Sixty adult female rats were randomly divided into 12 groups (n = 5). Group 1 was control. Group 2 received 5-weeks administrations of 10 mg/kg bodyweight of SA. Groups 3-6 received SA-dose for 2 weeks followed by 3-weeks posttreatments with MLF1-doses and AMF1-doses respectively. Groups 7-10 received only 5-weeks administrations of MLF1-doses and AMF1- doses respectively. Groups 11 and 12 received 5-weeks co-administrations of SA-dose with highdoses of MLF1 and AMF1 respectively. Drugs/extracts were administered orally. Liver histopathology (Heamatoxylin and Eosin) and ELISA concentrations of sera Melatonin and TNF-alpha were evaluated. Malondialdehyde (thiobarbituricacid assay) and p53 (ELISA) levels were evaluated in liver homogenates. Data were statistically analysed. Results showed normal liver histology in Groups 1-12. Post-treatments of SA-induced toxicity with MLF1 and AMF1 resulted in significant (P≤0.05) and non-significant decreased levels (P≥0.05) of Malondialdehyde, TNF-alpha and p53, but significant (P≤0.05) and non-significant increased Melatonin levels (P≥0.05) in Groups 3-12 compared with Group 2. MLF1 and AMF1 possess anticancer, antioxidant, pro-Melatonin, anti-inflammatory and hepato-protective potentials.
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    Regulatory effects of quercetin on testicular histopathology induced by cyanide in Wistar rats
    (Elsevier, 2021-07-24) Oyewopo, Adeoye; Adeleke, Opeyemi; Johnson, Olawumi; Akingbade, Adebanji; Olaniyi, Kehinde; Areola, Emmanuel; Tokunbo, Olorunfemi
    Several causes of infertility have been identified, and several papers have documented some compounds that cause infertility. One of the compounds reported to be toxic to the reproductive system is cyanide. In the management of infertility, various mechanisms ranging from synthetic drugs, natural products and supplements have been employed. Quercetin is an antioxidant supplement that has been used in the treatment of a variety of ailments. This work is aimed at investigating the role of quercetin in attenuating spermato-toxicity and testicular-histopathology induced by cyanide. Seventy-two (72) male wistar rat (weight 190 g ± 10 g) were divided into nine groups (n = 8) except for groups 4 and 5 with (n = 16). Group 1 (control) received physiological saline while Groups 2 and 3 received 0.5 and 1 mg/kg body weight (bwt) cyanide respectively for 56 days, groups 4 and 5 received 0.5 and 1 mg/kg bwt cyanide respectively for 30 days. At day 30, eight animals were sacrificed from Groups 4 and 5 and the remaining eight (8) rats were subdivided into groups (6 and 7) and were given 20 and 40 mg/kg bwt of quercetin respectively for twenty-six days. Co-administration of cyanide and quercetin at a dose of 0.5 mg/kg cyanide +20 mg/kg quercetin and 1 mg/kg cyanide +40 mg/kg quercetin were given to group 8 and 9 respectively for 56 days. Significant decreases in sperm parameters (count, motile and normal sperm) and increases in malondiadehyde concentration were observed in the cyanide treated groups. Testicular histoarchitecture showed few to no spermatozoa in the lumen of rats treated with cyanide. All these effects were attenuated by quercetin. In conclusion, quercetin regulates testicular histopathology induced by cyanide in Wistar rats. Data from this work suggests potential preventive or therapeutic applications of quercetin for individuals subjected to cyanide environmental pollution.