Browsing by Author "Oyewole, A. L."

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    EFFECTS OF THE ETHANOLIC EXTRACT OF BULBOSTYLIS COLEOTRICHA (HOCHST.EXA. RICH.) ON INFLAMMATION IN ADULT WISTAR RATS
    (Nigerian Society of Pharmacognosy, 2015-06-20) Owoyele, B. V.; Abioye, A. I. R.; Oyewole, A. L.; Ameen, Mubarak; Owemidu, I. O.; Adewusi, M. O.
    Bulbostylis coleotricha (family Cyperaceae) is a stem-tufted plant found in tropical region of Africa. It is locally used in treating various ailments among the African folks. Despite the high esteem this plant is held in Africa, there is dearth of scientific studies on B. coleotricha. This, in addition to the alarming increase in inflammatory diseases around the world, makes the present study to focus on the effect of the B. coleotricha extract on laboratory induced inflammation. Twenty-five adult Wistar rats, weighing between 225-287g were used for this experiment, and were randomly divided into five groups of five rats per group. The groups were labelled and treated as follow: Group 1, the negative control group, was treated with 10 ml/kg body weight (b. w.) of distilled water.Groups 2, 3 and 4, the treated groups, were treated with 25 mg, 50 mg and 100 mg/kg b. w. of ethanolic extract of B. coleotricha respectively. Doses were based on the local information on B. coleotricha usages. Group 5, the positive control group, was treated with 5mg/kg b. w. of indomethacin. In all the groups, the effects of all treatments were checked on induced inflammation using cotton pellet granuloma and carrageenan induced paw oedema model.The results in the two models used showed that ethanolic extract of B. coleotricha significantly inhibited inflammation in the treated groups. In the cotton pellet granuloma test the extract at the dose of 100 mg reduced granuloma formation from 30.2 ± 2.4 mg to 11.2 ± 2.6 mg reduced paw oedema from 7.0 ± 1.7 mm to 2.4 ± 0.8 mm (control is 100 mg/kg). In conclusion, this study established that Bulbostylis coleotricha plant is effective in the treatment of inflammation and can be explored in the treatment of various diseases with underlying inflammation.
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    Exposure to varied cage-size habitats alters pain sensitivity and inflammation-related biomarkers.
    (Elsevier, 2020) Oyewole, A. L.; Oyafemi, K. O.; Badmus, K. S. J. O.; Omoleye, K. S.; Abubakar, M. F.; Adeniyi-Raheem, O.; Amedu, A.; Lawal, D. L.; Ijiyode, A. O.; Yussuf, A. O.; Ishola, S. S.; Sulaimon, F. A.; Alli-Oluwafuyi, A.O.; Nafiu, A.B.; Akinola, O.; Olajide, O.J.; Amin, A.; Abdulmajeed, W.I.; Michael, O.S.; Adeyanju, O.A.; Ogunjimi, G.L.
    Background: Nature and size of rodent cages vary from one laboratory or country to another. Little is however known about the physiological implications of exposure to diverse cage sizes in animal-based experiments. Method: Here, two groups of male Swiss mice (Control group – Cage stationed, and Test group – Cage migrated) were used for this study. The cage-migrated mice were exposed daily to various cage sizes used across labora tories in Nigeria while the cage-stationed mice exposed daily to different but the same cage size and shape. At the end of the 30 days exposure, top-rated paradigms were used to profile changes in physiological behaviours, and this was followed by evaluation of histological and biochemical metrics. Results: The study showed a significant (p < 0.05) decrease in blood glucose levels (at 60 and 120 min of oral glucose tolerance test) in the cage-migrated mice compared to cage-stationed mice. Strikingly, peripheral oxi dative stress (plasma malondialdehyde) and pain sensitivity (formalin test, hot-and-cold plate test, and von Frey test) decreased significantly in cage-migrated mice compared to cage-stationed animals. Also, the pro-in flammation mediators (IL-6 and NF-κB) increased significantly in cage-migrated mice compared to cage-sta tioned mice. However, emotion-linked behaviours, neurotransmitters (serotonin, noradrenaline and GABA), brain and plasma electrolytes were not significantly difference in cage-migrated animals compared to cage stationed mice. Conclusion: Taken together, these results suggest that varied size cage-to-cage exposure of experimental mice could affect targeted behavioural and biomolecular parameters of pain and inflammation, thus diminishing research reproducibility, precipitating false negative/positive results and leading to poor translational outcomes.
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    Honey and levodopa comparably preserved substantia nigra pars compacta neurons through the modulation of nuclear factor erythroid 2-related factor 2 signaling pathway in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model
    (Korean Association of Anatomists, 2024) Sulaimon, F. A.; Ibiyeye, R. Y.; Imam, A.; Oyewole, A. L.; Imam, A. L.; Shehu, M.; Biliaminu, S. A.; Kadir, R. E.; Omotoso, G. O.; Ajao, M. S.
    : Parkinson’s disease (PD) affects about 8.5 million individuals worldwide. Oxidative and inflammatory cascades are implicated in the neurological sequels, that are mostly unresolved in PD treatments. However, proper nutrition offers one of the most effective and least costly ways to decrease the burden of many diseases and their associated risk factors. Moreover, prevention may be the best response to the progressive nature of PD, thus, the therapeutic novelty of honey and levodopa may be prospective. This study aimed to investigate the neuroprotective role of honey and levodopa against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced oxidative stress. Fifty-four adult male Swiss mice were divided into control and PD model groups of 27 mice. Each third of the control mice either received phosphate buffered saline, honey, or levodopa for 21 days. However, each third of the PD models was either pretreated with honey and levodopa or not pretreated. Behavioral studies and euthanasia were conducted 2 and 8 days after MPTP administration respectively. The result showed that there were significantly (P<0.05) higher motor activities in the PD models pretreated with the honey as well as levodopa. furthermore, the pretreatments protected the midbrain against the chromatolysis and astrogliosis induced by MPTP. The expression of antioxidant markers (glutathione [GSH] and nuclear factor erythroid 2-related factor 2 [Nrf2]) was also significantly upregulated in the pretreated PD models. It is thus concluded that honey and levodopa comparably protected the substantia nigra pars compacta neurons against oxidative stress by modulating the Nrf2 signaling molecule thereby increasing GSH level to prevent MPTP-induced oxidative stress.
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    Nig. J. Nat. Prod. and Med.
    (Nigerian Society of Pharmacognosy, 2015-05-01) Owoyele, B. V.; Abioye, A. I. R.; Oyewole, A. L.; Ameen, M. O.; Owemidu, I. O.; Adewusi, M. O.
    Bulbostylis coleotricha (family Cyperaceae) is a stem-tufted plant found in tropical region of Africa. It is locally used in treating various ailments among the African folks. Despite the high esteem this plant is held in Africa, there is dearth of scientific studies on B. coleotricha. This, in addition to the alarming increase in inflammatory diseases around the world, makes the present study to focus on the effect of the B. coleotricha extract on laboratory induced inflammation. Twenty-five adult Wistar rats, weighing between 225-287g were used for this experiment, and were randomly divided into five groups of five rats per group. The groups were labelled and treated as follow: Group 1, the negative control group, was treated with 10 ml/kg body weight (b. w.) of distilled water.Groups 2, 3 and 4, the treated groups, were treated with 25 mg, 50 mg and 100 mg/kg b. w. of ethanolic extract of B. coleotricha respectively. Doses were based on the local information on B. coleotricha usages. Group 5, the positive control group, was treated with 5mg/kg b. w. of indomethacin. In all the groups, the effects of all treatments were checked on induced inflammation using cotton pellet granuloma and carrageenan induced paw oedema model.The results in the two models used showed that ethanolic extract of B. coleotricha significantly inhibited inflammation in the treated groups. In the cotton pellet granuloma test the extract at the dose of 100 mg reduced granuloma formation from 30.2 ± 2.4 mg to 11.2 ± 2.6 mg reduced paw oedema from 7.0 ± 1.7 mm to 2.4 ± 0.8 mm (control is 100 mg/kg). In conclusion, this study established that Bulbostylis coleotricha plant is effective in the treatment of inflammation and can be explored in the treatment of various diseases with underlying inflammation