Browsing by Author "Oyedele, Julius Olusegun"

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    Genome sequencing of endophytic bacterial species associated with Datura stramonium and Sida acuta
    (Zagazig University, 2025) Zakariyah, Rahmat Folashade; Ajijolakewu, Kamoldeen Abiodun; Ayoola, Saheed Abiodun; Ajadi, Ayobami Elias; Kazeem, Muinat Olanike; Oyedele, Julius Olusegun
    Background: Endophytes are ubiquitous microbes that colonize plants’ tissues without causing any harm to the host plants, but rather, they confer several adaptable characteristics on them. They could be of bacterial, fungal, algal, archaeal and actinomycetes origins. Endophytes also produce useful bioactive metabolites some of which are potent antimicrobials. In this study, bacterial endophytes were isolated from the leaves of Datura stramonium and Sida acuta, both of which are important medicinal plants. Methods: Freshly cut leaves were collected, rinsed and sequentially sterilized. The sterilized leaves were then cut into smaller pieces and incubated onto extract-seeded nutrient agar medium augmented with antifungal drug. Pure distinct bacterial colonies were morphologically and biochemically characterized. Molecular characterization of the strains was done using the 16S rRNA while phylogenetic evolutionary analysis was carried out using the maximum likelihood method and Tamura-Nei model with MEGA 11 software. Results: A total of 7 bacterial endophytes were isolated and identified, 3 from D. stramonium and 4 from S. acuta. 16S rDNA genome sequencing of the strains revealed that isolates from D. stramonium were Pseudomonas aeruginosa, Atlantibacter hermannii and Enterobacter roggenkampii while those isolated from S. acuta were identified as Pseudomonas aeruginosa, Pseudomonas monteilii, Enterobacter pseudoroggenkampii and Aeromonas veronii. Conclusion: The outcome of this research has indicated that numerous strains of endophytic bacteria inhabit leaves of plants. It is therefore essential to exploit these endophytic bacteria for their potentials to produce bioactive metabolites and other valuable antimicrobial products.
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    A review of the ethnomedicinal, antimicrobial, and phytochemical properties of Musa paradisiaca (plantain)
    (2021) Ajijolakewu, Kamoldeen Abiodun; Ayoola, Saheed Abiodun; Agbabiaka, Tariq Oluwakunmi; Zakariyah, Rahmat Folashade; Ahmed, Risikat Nike; Oyedele, Julius Olusegun; Sani, Al-hassan
    Background: More people—in both developing and developed countries—now use, and are favourably disposed to, traditional medicine. Musa paradisiaca (plantain) is used extensively in traditional medicine across continents. In this review, we investigated the scientifc justifcation of this extensive usage. Main body: Generally, several studies validate usage in infectious diseases, but limited antiviral and in vivo reports exist. The mechanistic elicitation of antimicrobial activity has similarly not been ascertained. Contrarily, data abound from rigorous studies on physiological conditions. Activity across categories is tied to the potent phytosterols duo of stigmasterol and β-sitosterol; and the triterpenes viz. cycloeucalenone, 24-methylene-cycloartanol, and 31-norcy clolaudenone; present in plantain. Toxicity studies, while fnite, suggest general safety and tolerance. Conclusions: Findings in the studies reviewed projects plantain as a veritable source for drug bioprospecting that will be of beneft to scientifc research and pharmaceutical industries