Browsing by Author "Onigbolabi, O. G."
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Item Comparative Analysis of the Protective Potentials of Nigella sativa and Lepidium meyenii on Alcohol-induced Testicular Toxicity in Adolescent Wistar Rats(The Anatomical Society of Nigeria, 2024) Adana, M. Y.; Towoju, A. M.; Alade, O. E.; Oluwasegun, B. O.; Adewale, D. A.; Onigbolabi, O. G.; Ajao, M. S.Alcohol, a psychoactive drug, is soluble in both water and lipids, due to which it can diffuse to all tissues and affect the normal functioning of the body. Gonadal toxicity is reported as one of the side effects of its long-term consumption. This study examined the possible comparable protective potentials of Lepidium meyenii (LM) and Nigella sativa oil (NSO) on the biological integrities of the testes after exposure to excessive alcohol. Thirty-six adolescent Wistar rats (60– 150 g) were randomly divided into six groups and treated orally for 56 consecutive days. Group treatments included normal saline for the control group, 40% alcohol, LM, NSO, LM+alcohol, and NSO+alcohol. On the 57th day, samples were collected to assess reproductive hormones, sperm analysis, testicular histology and proliferating cell nuclear antigen (PCNA) expression. The results revealed that excessive alcohol consumption affected the structural integrities of the testes by depleting the mature sperm cell population and actively dividing PCNA immune-reactive cells. The treatment with Lepidium meyenii does not show any significant protective effects on alcohol-induced structural distortion of rats’ testes. However, NSO promises to be effective in protecting against alcohol-induced changes.Item Newbouldia Laevis Enhanced Bcl-2 Expression and Germinal Epithelial Proliferation in adolescent Wistar rats(2025-04) Adana, M. Y.; Adebayo, A. D.; Aina, S. A.; Nathan, Q. R.; Onigbolabi, O. G.; Ajao, M. S.Background: In traditional medicine, numerous plant extracts have been used as infertility treatments to enhance chances of procreation over the years. The use of Newbouldia laevis as an adjuvant has been shown in experimental animal models to have the ability to either stimulate or suppress male reproductive processes at specific dosages. Th study aimed to compare the effects of graded dosages of Newbouldia laevis and zinc on male fertility. Materials and Methods: Thirty-six male divided into six groups of five adolescent rats each. They were treated with normal saline, Zinc, and different doses and and duration , viz: low-dose short-term (LS) and low-dose long-term (LL), and high-dose long-term (HL) the motility and population groups when compared to other groups. Conclusion/Recommendations: Wistar rats, weighing between 55 - 125 g were randomly high-dose short-term (HS) Newbouldia laevis at for a period of 28 days , for of 56 days . The semen , histomorphometric analysis, and Bcl-2 expression, a protein that controls apoptosis, were studied to assess the testicular health in experimental animals. Results: The results demonstrated that high doses of Newbouldia laevis parameters impaired semen parameters, particularly of sperm cells, irrespective of treatment duration. The population of germinal epithelial cells was unchanged . However, t he expression of Bcl-2 The study suggests that was reduced in the high dose (HS and HL) moderate use of period may have beneficial effects on male fertility potential Newbouldia laevis extract for a limited with effects comparable to those of Zinc.Item Oral thymoquinone modulates cyclophosphamide‐induced testicular toxicity in adolescent Wistar rats(Wiley, 2022) Adana, M. Y.; Imam, A.; Bello, A. A.; Sunmonu, O. E.; Alege, E. P.; Onigbolabi, O. G.; Ajao, M. S.Cyclophosphamide (CYP) is an effective anti-cancer drug that is widely accepted, but it is not devoid of unintended toxic effects. Gonadal toxicity is reported as one of the side effects of its long-time use. This study examined the effects of thymoqui none (TQ) on the biological integrities of the testes after cyclophosphamide expo sure. Thirty adolescent male Wistar rats (100–110 g) were divided into six groups (n = 5), receiving normal saline (NS), 20 mg/kg of CYP (CYP), 5 mg/kg of TQ (TQ5), 10 mg/kg of TQ (TQ10), 20 mg/kg of CYP and 5 mg/kg of TQ (CTQ5), and 20 mg/ kg of CYP and 10 mg/kg of TQ (CTQ10) respectively. On the 22nd day, blood, semen and testicular samples were collected for the assay of serum reproductive hormones (follicle-stimulating (FSH) and luteinizing (LH) hormones), semen analysis and testicu lar histology and proliferating cell nuclear antigen (PCNA) expression. The results re vealed that CYP exposure affected functional and structural integrities of the testes, by depleting sperm count and motility, testosterone, LH, spermatogenic and mature sperm cell population, Leydig cells and PCNA immunoreactive proliferating cells. TQ interventions were able to reverse all cytotoxic CYP impacts, but with differential activities on the hormonal concentrations, specifically LH and FSH. Cumulatively, thymoquinone may be a potent agent against cyclophosphamide effects on the physi ological, regeneration and histological integrities of the testes, as observed in this study.Item Protective Potential of Thymoquinone on Cyclophosphamide-Induced Hepatotoxicity in Rat(National Association of Specialist Medical Doctors in Academics in Nigeria, 2022) Adana M. Y.; Opabunmi, E. T.; Sunmonu, O. E.; Bello, A. A.; Onigbolabi, O. G.; Imam A.; Ajao, M. S.Background: Cyclophosphamide (CYC) has been known as an anticancer drug with several side effects on various organs such as the liver. In this study, the hepatoprotective properties of thymoquinone (TQ) were tested to decrease the damaging effects of Cyclophosphamide on the liver. Objective: To study and observe the possible ameliorative effect of thymoquinone on cyclophosphamide-induced hepatic toxicity in Wistar rats. Methods: Thirty Wistar male Adolescent (8 – 9 weeks old) rats weighing 70g – 150g were divided into Six groups; 5 rats per group (n=5) and treated orally for 21 consecutive days. Group A served as the control, and the rats in this group received normal saline. Group B was treated with 20mg/kg of cyclophosphamide. Group C was treated with 5mg/kg of Thymoquinone. Group D was treated with 10mg/kg of Thymoquinone. Group E was treated with 20mg/kg of cyclophosphamide and 5m/kg of Thymoquinone while Group F animals were treated with 20mg/kg of Cyclophosphamide and 10mg/kg of Thymoquinone. Results: The Cyclophosphamide treated group showed significant decreases in the body and liver weight, distorted hepatic cytoarchitecture, and reduction in hepatic function whereas co-treatment with TQ, showed protective effects on Cyclophosphamide-induced hepatotoxicity damage. Conclusion: These results suggest that the administration of Thymoquinone may have a protective potential on Cyclophosphamide induced hepatotoxicity.