Browsing by Author "Onanuga, Ismail Olashile"
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Item Effects of ascorbic acid on garlic-induced alterations in semen parameters of Wistar rats.(The Tropical Journal of Health Sciences, 2011) Omotoso, Gabriel Olaiya; Onanuga, Ismail Olashile; Jimoh, AAGItem Garlic consumption alters testicular histology and anti-oxidant status in Wistar rats.(The Tropical Journal of Health Sciences, 2012) Omotoso, Gabriel Olaiya; Oyewopo, AO; Onanuga, Ismail Olashile; Enaibe, BUGarlic is known for its cell and tissue-protective functions. This study examined the effects of aqueous garlic extract on the histology of the testes of Wistar rats and erythrocyte superoxide dismutase (SOD) activity. Twenty-four (24) rats with an average weight of 116 g were used. They were randomly grouped into three: Group A Control: was given distilled water; Group B: was given 500 mg/kg/d aqueous garlic extract; and Group C: received 1000 mg/kg/d aqueous garlic extract; all for 28 days. Findings revealed progressive depletion in sperm cell population, reduction in size of seminiferous tubules, and widening interstitial space. SOD activity was significantly reduced in both treated groups in a dose-dependent fashion. These histological observations and the depletion in endogenous anti-oxidant status associated with the administration of garlic could result in significant affectation of male reproductive functions.Item Liver enzymes derangement and the influence of diet in animals given oral albendazole(Nigerian Medical Journal, 2013) Omotoso, Gabriel Olaiya; Enaibe, BU; Oyewopo, AO; Onanuga, Ismail OlashileBackground: Albendazole is used as an anthelmintic in the treatment of some parasitic infections. This study determined how the effects of albendazole on liver enzymes are influenced by diet. Materials and Method: Thirty adult male Wistar rats of mean weight 304.12 ± 11.34 g were randomly grouped into five: Group A: Control, was given rat pellets and water only; Group B received 15 mg/kg/d of albendazole while fasting; Group C received 15 mg/kg/d of albendazole with fatty meal; Group D received 15 mg/kg/d of albendazole with normal diet (rat pellets); and, Group E received 30 mg/kg/d of albendazole with normal diet (rat pellets); they were given orally for 3 consecutive days. The animals were sacrificed thereafter and blood samples obtained for quantitative study of the serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Results: Significant elevation in the serum levels of the transaminases especially in animals which were on their normal diet (rat pellets), while ALP was either reduced or increased based on dietary factors. Conclusions: Oral administration of albendazole before meal or with a fatty diet could help limit severe elevation of liver enzymes associated with its use, while still ensuring optimal efficacy.Item Testicular alteration in overdosage of azathioprine: a histological and histochemical study in Wistar rats(African Journal of Cellular Pathology, 2014) Onanuga, Ismail Olashile; Ibrahim, R B; Amin, A; Omotoso, Gabriel OlaiyaAim: The use of Azathioprine (AZA) in the prevention of organ rejection during transplantation has been documented for different organs of the body. In this study, we investigated the effects of azathioprine on the integrity of the testis of Wistar rats using histological and histochemical techniques. Methods: Eighteen adult male Wistar rats with mean weight 210±2.65 g were randomly assigned into three groups. Group I (control) received 1 ml of normal saline “per os” (p.o), group II animals received 10 mg/kg AZA (p.o) while group III animals received 20 mg/kg AZA (p.o). Treatment lasted for 21 days. Twenty-four hours after treatment, animals were sacrificed; their testes excised, weighed and fixed in Bouin’s fluid for histological evaluation using Haematoxylin and Eosin while histochemical studies were carried out using Gordon and Sweets, and Masson’s Trichrome staining techniques. Testicular homogenate was used to assay for testosterone level. Results: Treatment with Azathioprine reduced testosterone level while the histological and histochemical findings of the testicular sections revealed cytoarchitectural distortions and reduced staining intensity of collagen and reticulin connective tissue fibers in AZA treated animals compared to the control group. Conclusion: Results from the study reveal that the immunosuppressive drug azathioprine when used at a concentration above 10 mg/kg disrupts the testicular integrity of adult Wistar rats.