Browsing by Author "Olurinola, P.F"

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    Clinical Bacterial Isolates from Hospital Environment as agent of Surgical Wound Nosocomial Infection.
    (Faculty of Pharmaceutical Sciences, University of Jos, 2010) Giwa, A; Attah, R.F; Ibrahim, Y.K.E; Olurinola, P.F; Akanbi II, A.A; Sanni, A.A
    The relationship between bacteria isolated from the hospital environment and those from wounds of operated patients was investigated to determine the causal agents of surgical site nosocomial infections. The study was carried out on bacterial species isolated from the theatre, surgical ward and patients’ surgical wounds in a tertiary health institution in Nigeria. Bacteria were isolated from the air, floor and patients’ surgical wounds in the theatre and surgical ward by using MacConkey agar, Chocolate agar Nutrient agar and Peptone water broth as isolating media. Plasmid sizes and bands of selected twenty (20) of the isolates were determined by electrophoresis analysis to determine their relatedness. The bacterial species isolated were: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Proteus mirabilis, Proteus vulgaris, Citrobacter freundii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Serratia marscenses. The result of electrophoresis showed that some of the isolates from the hospital environment and surgical sites have the same number of bands and molecular weight. It was concluded that isolates from the hospital environment with the same numbers of bands and molecular sizes with those isolated from patients wounds in the same hospital environment are of the same strain, and must have come from the same source, and therefore are likely to be responsible for the surgical wound infections observed in the patients studied.
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    Evaluation of Hospital Disinfection as a means of controlling Endemic Nosocomial Pathogens in a University Teaching Hospital in Nigeria
    (Journal of Pharmacy and Bioresources, 2010) Giwa, A; Atata, R.F; Ibrahim, YKE; Olurinola, P.F; AKANBI II, A.A; SANNI, A. A
    Evaluation of hospital environment disinfection as a means of controlling endemic nosocomial pathogens in a University Teaching Hospital in Nigeria was evaluated. Disinfectant used in the Hospital was collected from the Infection Control unit and prepared in different concentrations. The isolated bacterial species from the hospital environment were exposed to graded concentrations of the disinfectants and the most effective concentration on each isolate was noted. This procedure was carried out in two successive years (2006 and 2007). Killing rate of the isolates that were resistant to the disinfectants was also carried out and likely effective exposure time was determined. The following bacterial species were isolated: Staphylococcus epidermidis, Klebsiella Pneumoniae, Klebsiella spp., Bacillus subtilis, Enterobacter spp., Serratia marcescens, Pseudomonas aeruginosa, Pseudomonas spp., Escherichia coli, Serratia spp., Bacillus cereus, Citrobacter freundii, Proteus mirabilis, Staphylococcus aureus, Bacillus megaterium, Streptococcus pyogenes, and Streptococcus spp. Minimum Effective Dilution (MED) of the disinfectant on all isolates ranged from 1:300 to1:1000. Staphylococcus aureus and Pseudomonas aeruginosa were the most resistant isolate with MED of 1:400 and 1:300 respectively. Result of killing rate on the two most resistant isolates showed that Staphylococcus aureus and Pseudomonas aeruginosa required 80 and 120 minutes of exposure respectively to the disinfectant to bring about almost total killing of these resistant isolates. The results show that improper disinfections, degradation of disinfectant and lack of routine standardization of disinfectants are responsible for failure of chemical disinfection as a means of controlling nosocomial infections in the hospital.
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    : Prevalence of surgical site nosocomial infection in tertiary health care institution in Nigeria
    (Seiknow publication Ltd, 2013) Atata, R. F; Ibrahim, Y.K.E; Olurinola, P.F; Adigun, I.A.; Giwa, A.; Abdul, I.F.; Akanbi, A.A.