Browsing by Author "Olayinka, Bolaji U."

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    A Study on Endophytic Fungi, Proximate and Chemical Compositions of a Local Variety of Mango Fruit (Mangifera indica L.)
    (University of Ruhuna, Sri Lanka., 2018) Garuba, Taofeeq; Olayinka, Bolaji U.; Abdulkareem, Khadijah A.; Bello, M. O.; Olaleye, Faith Y.
    Mango (Mangifera indica L.) is an important tree which produce edible fruits. Different varieties of mango are available and well-studied. However, little attention has been shown to popular Ogbomoso variety especially for its endophytes and chemical profile. This work aimed at identifying endophytic fungi and carrying out proximate analysis as well as phytochemical analysis of the fruit. Potato Dextrose Agar was used as a medium for the isolation of fungi from the homogenized pulp. Proximate analysis was carried out using standard method of Association of Analytical Chemistry (AOAC). Fourier Transform Infrared Spectroscopy (FTIR) was done to determine characteristic peaks and functional groups of compounds using Thermo Scientific Nicolet iS5 with iD1 transmission. Gas Chromatography- Mass Spectrometry (GC-MS) was used to study the chemical profile of the sample. Aspergillus niger. A. flavus and Rhizopus stolonifer were the identified endophytes. Moisture and lipid were observed to be highest (76.87%) and lowest (0.38%) respectively in the sample. The amount of carbohydrate was 18.93%. The FTIR result revealed the presence of NH2, OH, CH2, C=O (carboxylic) and N-H 1o and 2o amine as important functional groups. A total of 23 compounds were found in the methanolic extract of the sample where Imidazolidin-2-one (18.43%) was the principal compound. N, Ndibenzylhydroxylamine (15.36%), Methyl hexadecanoate (7.33%), Hexadecamethylcyclooctasiloxane (4.51%) were also present in considerable quantities. It is confirmed that the homogenized pulp of Ogbomoso variety of mango fruit is rich in phytochemicals that are useful especially in pharmaceutical industries.
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    Comparative Biodiversity Assessment of Weed Species in Monocropping Plantations of University of Ilorin, Nigeria
    (University of Ghana, 2020) Olayinka, Bolaji U.; Adeyemi, Sherif B; Abdulkareem, Khadijah A.; Olahan, Ganiyu S.; Lateef, Azeez A.; Garuba, Taofeeq; AbdulRahaman, A. A.
    The present study investigates the weed species diversity in four plantations of university of Ilorin, Ilorin, Nigeria using quadrat method. The survey revealed two major life cycles (annual and perennial) and recorded a total of 88 weed species belonging to 32 families. Four species (Azadirachta indica, Daniellia oliveri, Desmodium tortuosum, and Tridax procumbens) were common in all the surveyed plantations while thefamily Fabaceae was the most dominant. The abundant weed species analysis showed a high importance value index and were more adapted to the plantations. Diversity analysis revealed high species richness in the sugarcane plantation. The non-canopy nature of the plantation, soil structure as well as ability to coexist with many other species may underscore the reasons for this pattern of diversity. The evenness and similarity indices between and across the plantations were generally low, thus, indicating varying diversity. As a result of the recorded variation in weed composition between and across the plantations, the study has provided an insight on the pattern of weed diversity in the studied plantations. The study recommended that the most abundant weed species populations be checked for the plantations to thrive. Finally, there is an urgent need to conserve weed species that are not only rare in abundance but also showed great social and economic values
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    Germination of several groundnut cultivars in relation to incidence of fungi
    (Faculty of Science, Kaduna State University, 2017) Ahmed, O.; Olayinka, Bolaji U.; Garuba, Taofeeq; Etejere, E. O.
    This experiment is concerned with the germination of nine cultivars of groundnut grown in Nigeria in relation to incidence of fungi. The cultivars were NHK 5V8, NUTII 288, Samnut 10, 11, 21, 22, 23, 24 and MK 373. Germination potential was assessed after 10 days of planting in petri-dishes. Parameters such as seedling vigour and electrical conductivity were assessed. Potato dextrose agar (PDA) was used to isolate the fungi grown in each seed types and the fungi were identified. Four species of fungi: Aspergillus niger, A. flavus, Fusarium sp and Penicillium sp were isolated in all the nine cultivars at varying degree except Aspergillus niger that was absent in Samnut 24. Fusarium sp. was preponderant in NUTII288, Samnut 22, 23 and 24. Whereas A. flavus was highest in NHK5V8, MK 373 and Samnut 21. The species such as A. niger and Penicillium sp were respectively high in occurrence in Samnut 10 and 11. Samnut 23 showed highest percentage germination followed by Samnut 24 and 21 (100-90%). All other cultivars had percentage germination between 70-80%. The results of speed, ability and seedling vigour followed similar trend as recorded for percentage germination. Seeds with higher vigour showed lower conductivity test as compared to those with low vigour. Generally, cultivars with high germination potential showed low incidence of fungal attack
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    Molecular Characterisation of Potential Crop Pathogens Associated with Weeds as Endophytes in Unilorin Plantations, Nigeria
    (College of Science for WomenUniversity of Baghdad, 2022) Lateef, Azeez A.; Garuba, Taofeeq; Abdulkareem, Khadijah A.; Olayinka, Bolaji U.; Olahan, Ganiyu S; Adeyemi, Sherif B.; AbdulRahaman, Abdullahi
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    Molecular Characterisation of Some Accessions of Corchorus olitorius
    (Faculty of Science, Federal University Birnin Kebbi, 2019) Abdulkareem, Khadijah A.; Sidiq, K. O.; Olayinka, Bolaji U.; Lateef Azeez A.; Garuba, Taofeeq; Adeyemi, Sherif B.; Sagaya, Abdulkadir
    Nigeria has several native leafy vegetables that have been neglected. Corchorus olitorius belongs to this group. Genetic diversity was studied in five accessions of the plant using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS - PAGE). The aim was to determine the relationship (differences and similarities) among them. Sixteen polypeptide bands were obtained ranging from 12.29 KDa to 170 KDa indicating variations in the banding patterns of the accessions. The unweighted pair group method with arithmetic algorithm (UPGMA) dendrogram grouped the five accessions into three clusters with four groups showing 60% of the accessions in one group. The greatest similarity (96%) observed was between NGB00196 and NGB00194 while the lowest similarities (32%) NGB00191 and (40%) NGB00187. Adopting this technology can be useful in plant variety identification and registration of new plant varieties. NGB00191 was observed to be distantly related to the other accessions hence could be combined in a breeding programme.