Browsing by Author "Olayinka, B.U"

Now showing 1 - 14 of 14
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    Alleviation of Drought Stress in Tomato (Solanum lycopersicon) Using Organic Mulch
    (Nigerian Institution of Professional Engineers and Scientist, 2022-05-29) Isiaka, K; Oluwasogo, I.I; Yusuf, S.Y; Lawal, M.T; Abdulkareem, K.A; Olayinka, B.U
    Despite the importance of tomato in human nutrition, occurrence of drought stress constitutes a major bane to its bountiful production. Therefore, this experiment was conducted to determine the effectiveness of organic mulch in improving growth, yield and quality of water stressed tomato. The experiment was laid out in randomized complete block design (RCBD) with three replications. The six treatments used in the experiment were normal irrigation with zero mulch, drought stress with zero mulch and drought stress with mulch at the rates of 10tons/ha, 15tons/ha, 20tons/ha and 25tons/ha. The organic mulch material used was maize husk and the tomato variety tested was Platinum 107 F1. Water deficit treatment was imposed at flowering stage (five weeks after transplanting (5WAT)) by applying 50% field capacity as irrigation water. Data were collected on plant height, number of leaves per plant, number of branches per plant, number of flowers per plant and mass of fruit yield per plant. Furthermore, leaf proximate compositions (crude fat, crude protein, crude fibre, ash content and dry matter) and leaf chlorophyll content were determined. All the data collected were subjected to analysis of variance (ANOVA) and significant means were separated using least significant difference (LSD) at 5% probability level. Results from this research work showed that stressed plants without mulch application had a drastic reduction in all the measured parameters. The plants subjected to drought and treated with 20tons/ha mulch showed higher plant height, number of branches and yield as well as chlorophyll, fiber and ash content compared to the plants treated with 10tons/ha, 15tons/ha and 25t/ha mulch. It is, therefore, concluded that 20tons/ha mulch be used for alleviating drought stress condition in Platinum 107 F1 tomato variety in the study area when all the conditions in this research are maintained.
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    Composition of Coconut (Cocos nucifera L. Cv. Fiji Dwarf) Edible Endosperm and Physicochemical Properties of its Oil
    (Federal University of Kashere-Nigeria, 2021-08-15) Abdulkareem, K.A; Olayinka, B.U; Lawal, A.R.; Adeniji, O.A; Ibuowo, M.B; Jacob, O.E; Abdulra'uf, L.B; Kareem, I.; Aboyeji, O.O; Mustapha, O.T; Etejere, E.O
    Coconut (Cocos nucifera L.) is one of the most important oil producing crops in the world. It has enormous industrial and health benefits. To affirm the foregoing, the proximate and physicochemical constituents of the oil extracted from the edible endosperm were determined. The results showed that percentage moisture, protein, ash, crude fat, crude fibre and carbohydrate had respective values of 42.39 %, 6.21 %, 1.04 %, 17.18 %, 8.95, and 23.69 %. The edible mesocarp has high amount of mineral elements such nitrogen, potassium, calcium and phosphorus. Whereas, elements such as sodium, manganese, magnesium, copper and chlorine occurred in low quantity. The physicochemical properties of the oil showed a refractive index of 1.46, specific gravity of 0.92 g/cm3, acid value of 2.15 %, free fatty acid of 1.08 %, saponification of 125.980 mgKOH/g and iodine value of 2.89 indicating that the oil is good for consumption. Given, the results of proximate and mineral compositions, the edible endosperm could therefore be considered as good source of energy and alternative source of dietary supplement for human nutrition.
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    DNA Barcoding of Vernonia amygdalina using ITS and RPOC 1 Multi Loci Gene Regions
    (Faculty of Science, Federal University Birnin Kebbi, 2023-12) Abdulkareem, K.A; Elebiyo, P.T; Olayinka, B.U; Tiamiyu, B.B; Kareem, I; Danzaki, M.M; Mustapha, O.T
    Vernonia amygdalina is one of the most well-known plants found in Africa and Asia and the most cultivated species of the genus Vernonia. The knowledge of how living and extinct species are related to one another supports much of evolutionary Biology. Therefore, this study was aimed at carrying out the molecular identification of Vernonia amygdalina using ITS and RPOC1 regions. In this study, the identification and phylogenetic analysis of Vernonia amygdalina was done through amplification of the DNA using ITS and RPOC1 primers. The ITS and RPOC1 sequences were compared with the existing sequences in the NCBI GenBank using BLAST searches then the sequences were aligned and maximum likelihood and parsimony trees were constructed using MEGA11. The result showed that ITS sequence length is 627bp while the RPOC1 sequence is 422bp. The blast result for ITS primer sequence showed 93% - 96% similarity against 6 accessions, 70% - 74% against 8 accessions, 64% - 69% against 6 accessions, and 82% similarity against 1 accession. Whereas the RPOC1 primer sequence gave 96% - 99% similarity against all accessions in the BLAST results. Vernonia amygdalina, Hirpicium diffusum, Cyanthillium cinereum, Sonchus ustulatus, Olearia odorata, and Olearia laxiflora were observed as out groups for their respective trees with the RPOC1 primer producing the highest percentage with all BLAST matches ranging from 96% to 99%. This indicates that the RPOC1 primer produced higher identification to species level.
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    Effect of Planting Methods on Growth and Yield of Groundnut Cultivars
    (Agricultural Research Communication Centre, 2021-01) Olayinka, B.U; Abdulbaki, A.S; Mohammed, R.T; Alsamadany, H; Murtadha, R.B; Alzahrani, Y; Abdulaziz, A; Abdulkareem, K.A; Etejere, E.O
    Background: The low yield in groundnut which can be partly attributed to poor land preparation methods necessitated the need for alternative strategy of land preparation that will promote higher productivity in groundnut farming. Methods: A field experiment was carried out in two separate locations between May and August in 2016 to assess the effect of planting methods on growth and yield of groundnut cultivars. Result: The results revealed that groundnut crop sown on modified ridges and furrow with narrow furrow planting recorded significantly higher plant height, number of leaves, 50% flowering, yield attributes and yield when compared to other methods of planting All the groundnut cultivars responded positively to all planting methods except those grown on flat surface without beds. However, modified ridges and furrow with narrow furrow planting proved to be the best method.
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    Effect of salinity stress on growth, yield and nutritional qualities of two okra varieties
    (Agricultural Academy of Bulgaria, 2020) Kareem, I; Dauda, Z.I; Kareem, S.A; Abdulmaliq, S.Y; Adekola, O.F; Abdulkareem, K.A; Olayinka, B.U; Abdulaziz, A; Alasinrin, S.K; Usman, M; Mahamoud, A,R
    The objective of this study was to determine the effect of salt stress on the growth yield and nutri tional quality of two okra varieties. The experiment was laid out in randomized complete block design (RCBD) with three replications. Clemenson spineless NHA-e varieties of okra were the two varieties tested. The treatments used comprised four salinity levels (0 mM, 25 mM, 50 mM, and 75 mM) us ing sodium chloride as the salinity source. The varieties were assessed using plant height, number of leaves, leaf area, days to first flowering and fruit weight. Furthermore, moisture, carbohydrate, crude protein, crude fat, ash and crude fiber contents were used to assess the fruit nutritional qualities. The results showed that Clemenson spineless variety had better tolerance to salinity stress in plant height, days to flowering, fruit mass, ash content and crude protein content. However, NHA-e variety was more tolerant in fruit dry matter, crude fibre content, carbohydrate content and ether extract content. It is concluded that Clemenson spineless was more tolerant to salinity stress than NHA-e variety. Therefore, Clemenson spineless variety should be used in saline areas of Ilorin which is the northern guinea savannah zone of Nigeria and places with the same edaphic and climatic attributes
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    EVALUATION OF CALCIUM CARBIDE'S CYTOTOXIC EFFECTS ON ONION (Allium cepa)
    (Federal University Dutsinma, Nigeria., 2023-06-02) Abdulkareem, K.A; Olayinka, B.U; Sulyman, R.A; Kareem, I; Danzaki, M.M; Mustapha, O.T
    The purpose of this investigation was to see if Calcium Carbide (CaC2) had any harmful effects on onions (Allium cepa L.). Allium cepa root tips were grown in various concentrations of Calcium Carbide (CaC2) (0.25g, 0.50g, 0.75g, and 1.00g)/ 250ml, with distilled water serving as a control. For cytological tests, the root tips of Allium cepa growing in the treatments and control were removed daily between 7:30am and 8:30am. Pretreatment, fixation, hydrolysis, squashing, and staining of cells for mitotic investigations were performed, and data on cytological parameters were collected using a light microscope at a magnification of X40. The mitotic index (MI) and karyotype analysis were used to assess the data collected on these parameters. The results of this study showed that root tips treated with 0.25g of Calcium Carbide (CaC2) have a mitotic index of 45.83 and the rate of cell division decreases with an increase in the concentration of Calcium Carbide (CaC2) as the cell divides the mitotic index dropped sharply. These finding indicated that Calcium Carbide is a strong mitotic inhibitor and could give rise to mitotic abnormalities with increase in concentration and also reduced cell division. We therefore recommended that there is need for further investigation using lower concentrations of Calcium Carbide as well as other mutagenic substances in order to ascertain their effect on the chromosomal behavior
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    GENETIC VARIABILITY VIA PROTEIN ELECTROPHORESIS AMONG SOME NIGERIAN ACCESSIONS OF PIGEON PEA (CAJANUS CAJAN)
    (The Institute of Biology, Bucharest, Romania, 2021) Abdulkareem, K.A; Olayinka, B.U; Danzaki, M.M; Idris, R; Kareem, I; Aziz, A; Sagaya, Q; Mustapha, O.T
    Purpose: Lack of genetic variability is a limitation to pigeon pea improvement. Hence the need to study the genetic variability of five accessions of pigeon pea with the view of isolating those that are with high yield and early maturity. Research Method: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) was used in the protein extraction and genetic diversity was estimated using Paleontological Statistics (PAST). Findings: A total of 24 protein bands were obtained ranging from 10kDa to 170 KDa. The protein band with the highest molecular weight was generated in accession NG/AO/MAY/09/021, NG/AO/11/08/108, NG/SA/07/0180 and NG/SA/07/208 while lowest molecular weight i.e 10 KDa was generated in NG/AO/MAY/09/021. Highest similarity index was recorded (45.16%) in NG/SA/07/0180 and NG/SA/07/208 and lowest (13.04%) in NG/SA/07/0180 and NG/AO/MAY/09/021. The bands showed variability based on intensity and presence or absence of any of them among the accessions. Jaccard’s similarity separated the 5 accessions into two clusters at an UPGMA similarity coefficient range of 0.2 to 1. Originality/Value: Accession NG/AO/MAY/09/021 occupies a distinct position hence could be combined with other accessions in a breeding programme.
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    INFLUENCE OF SEED PRIMING ON YIELD, MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERISTICS OF WATER-DEFICIT STRESSED LOWLAND RICE
    (West University of Timisoara, Romania, 2021-08-28) Kareem, I; Ismail, M.R; Puteh, A.B; Kareem, S.A; Lawal, O.I; Adekola, O.F; Faseyi, A.F; Olaniyan, J.O; Yusuf, S.Y; Lawal, M.T; Abdulkareem, K.A; Olayinka, B.U; Abdulaziz, A.; Ahmed, O; Baba, H.S; Kuranga, H.A; Lawal, Q.O; Musa, A.I; Oyelade, H.Y
    One of the potential ways of increasing rice production to cater for ever increasing population of Malaysia is to extend the production area of lowland rice through its production as upland rice. Therefore, this research was conducted to determine the effects of seed priming in alleviating water deficit stress in lowland rice produced as upland rice. The treatments used were 100mM calcium chloride dihydrate for duration of 48hours and temperature of 25oC, 40% w/v polyethylene glycol (PEG) 6000 for a duration of 48hours and temperature of 25oC, 100ppm kinetin for a duration of 24hours and temperature of 4oC, 200ppm methyl jasmonate for a duration of 24hours and temperature of 4oC, stressed control (unprimed seeds) and the unstressed control (unprimed seeds with adequate irrigation). The experiment was laid out in randomized complete block design (RCBD) with three replications. Data were collected on classical growth parameters, germination pattern, gas exchange characteristics, yield and yield components. It was found that priming MR219 rice with PEG could enable the variety to be produced as upland rice with little yield reduction because PEG priming was the best in individual seed mass, final yield, harvest index and water use efficiency. It is, therefore, concluded that 48hours of priming with 40%w/v polyethylene glycol at 25oC be used for MR219 rice seeds whenever MR219 rice variety is to be cultivated as upland rice.
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    Molecular Characterization of Potential Crop Pathogens Associated with Weeds as Endophytes in Uniilorin Plantations, Nigeria
    (College of Science for Women/ University of Baghdad, 2022-05-20) Lateef, A.A; Garuba, T; Abdulkareem, K.A; Olayinka, B.U; Olahan, G.S; Adeyemi, S.B; Abdulrahaman, A.A
    Crop diseases are usually caused by inoculum of pathogens which might exist on alternate hosts or weeds as endophytes. These endophytes, cum pathogens, usually confer some beneficial attributes to these weeds or alternate hosts from protection against herbivores, disease resistance, stress tolerance to secondary metabolites production. This study was therefore carried out to isolate potential crop pathogens which exist as endophytes on weed species in the University of Ilorin plantations. Green asymptomatic leaves were collected from 10 weed species across the plantations, and processed for their endophytic fungi isolation. Isolates were purified into pure cultures and used for molecular identification using the internal transcribed spacer (ITS) region of the ribosomal DNA. Phylogenetic analysis of the fungal sequences using MEGA software revealed 9 fungal genera belonging to 13 species, with species in the genera Curvularia, Epicoccumand Daldinia occurring in more than one weed species, while other genera such as Alternaria, Fusarium, Chaetomium, Macrophomina, Arthrinium and Phomopsis occurred in just one weed species each. Daldinia eschscholtzii was isolated in this study as an endophyte from Loudetia arundinacea for the first time. This plant is very abundant in Nigeria and Africa where it is used majorly for thatching and feeding livestocks. This also represents the first endophytic fungi from the genus Loudetia. Potential relationship between the occurrences of these fungi as endophytes and as pathogens are discussed. These discoveries represent the first large-scale molecular identification and several first reports of endophytes from these weed species. These results also represent the first records of some of these fungi in Nigeria.
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    NPK partitioning, growth, yield and proximate composition of okra (Abelmoschus esculentus) under water deficit stress
    (Agricultural Academy of Bulgaria, 2020-04) Kareem, I; Faseyi, A.F; Saliu, A.K; Abdulmaliq, S.Y; Adekola, O.F; Abdulkareem, K.A; Olayinka, B.U; Abdulaziz, A.; Alasinrin, S.K; Hakeem, A.K; Usman, M.; Lawal, M.T; Ahmed, O; Lawal, O.I; Bello, W.B; Salami, T.B; Ayeleke, D.A; Azeez, K.O; Olaniyan, J.O; Affinnih, K.O; Olalekan, K.K
    This experiment was conducted to determine the effects of drought stress on growth yield, NPK partitioning and nutritional composition of okra. To achieve this objective, four levels of water deficit stress (daily (control), a day interval, two days interval and three days interval irrigation) were tested on 17Lucky19 (hybrid) okra variety in a pot experiment. The experiment was laid out randomized complete block design (RCBD) with three replications. Parameters used to determine the effects of water deficit stress were nitrogen, phosphorus and potassium contents of stems and leaves. Plant height, number of branches, number of leave, number of fruits, fresh fruit mass, dry straw mass, chlorophyll content, and proximate parameters (crude fat, crude fibre, crude protein and ash contents of the leaves) were also used. It was found that all the growth and yield parameters tested reduced with increase in water deficit levels. Similarly, all proximate parameters decreased with increase in water deficit levels with the exception of crude fibre which increased with increase in water deficit levels. In the same vein, nitrogen and potassium levels decreased with increase in water deficit levels in both leaves and stems. However, phosphorus levels in stems and leaves decreased with increase in water deficit levels. It is, therefore, concluded that 17Lucky19 is susceptible to water deficit stress. This implies that water deficit tolerant or resistant varieties should be used instead of 17Lucky19 whenever areas with irregular rainfall are to be used for cultivating this variety of okra.
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    Optimization of priming duration for rice production under drought stress
    (Agricultural Academy of Bulgaria, 2020-04) Kareem, I; Kareem, S.A; Abdulmaliq, S.Y; Adekola, O.F; Abdulkareem, K.A; Olayinka, B.U; Abdulaziz, A.; Yusuf, A.S; Kuranga, A.B; Magaji, U.; Lawal, M.T; Ahmed, O; Lawal, O.I; Bello, W.B; Salami, T.B; Ayeleke, D.A; Azeez, K.O; Olaniyan J,O; Affinnih, K.O; Olalekan, K.K
    The type of priming chemical and the duration of priming are important factors determining the performance of plants resulting from priming treatment. Therefore, this research was conducted to determine the effects of different priming agents and soaking durations on growth and yield of drought-stressed rice. To achieve this objective, a pot experiment was conducted where three priming agents (100 mM calcium chloride dihidrate (CaCl2), 40% (w/v) polyethylene glycol (PEG) 6000 and 100 ppm kinetin) combined with two soaking (priming) durations were tested on drought stressed rice plants in a 3x2 factorial experiment. The experiment was laid out in a completely randomized design (CRD) with three replications. Parameters on germination percentage, number of tillers, number of productive tillers, tiller effi ciecy, shoot fresh and dry weight, yield, seed length to seed width ratio (seed size), 100-seed weight and harvest index were taken. Also, data on photosynthetic rate, stomatal conductance, intercellular carbon-dioxide and transpiration rate and leaf water were taken. It was found that the highest yield was got when PEG was used for priming for 48 hours. It was found that number of tillers, shoot dry mass, 100-seed mass, rate of photosynthesis, stomatal conductance, transpiration and leaf area water were increased when seeds were primed for 48 hours with kinetin solution. However, tiller efficiency, harvest index and seed size were favoured by 24-hour priming with PEG while number of productive tillers, shoot fresh mass and grain yield were favoured by 48 hour priming with PEG. It was concluded that 48 hour priming with PEG was effective alleviate moisture stress in MR219 rice. This implies that to avoid wastage of priming chemicals and circumvent undue prolongation of priming period or duration which will result in harming the seeds (toxicity) and poor performance of the resulting plants, 40% (w/v) PEG 6000 should be used for 48 hours for priming MR219.
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    Phylogenetic Position of Nigerian Species of Curcuma longa (Zingiberaceae) in the Current Infrageneric Classification
    (The College of Natural and Applied Sciences, Fountain University, Osogbo, Nigeria, 2024) Tiamiyu, B.B; Lateef, A.A; Sagaya, A; Abdulkareem, K.A; Olayinka, B.U; Ayinla, A; Adeyemi, S.B; Amenu, S.G; Abdulrahaman, A.A; Mustapha, O.T
    Curcuma longa L. (commonly known as Tumeric) is the only species of the genus Curcuma found in Nigeria. It is of great economic importance to Nigeria, Africa, Asia, and other parts of the world, where it is widely used for ornamental and medicinal purposes, and as spices in food and beverages. However, the phylogenetic placement of the turmeric plant (C. longa) in Nigeria is far from being fully resolved, hence the need for this study. The rhizomes of turmeric were collected at the Forestry Research Institute of Nigeria, Ibadan, Oyo state. Genomic DNA was extracted, followed by the amplification of the ITS and psbA-trnH regions. Phylogenetic analysis was conducted using the Maximum likelihood method. The result resolved the phylogenetic position of Nigerian species and supported existing subgenera classification into three clades, all with high bootstrap support for the three clades. The result of this study supports the subgenera classification of the genus and further reveals the phylogenetic position of C. longa.
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    Stressful Effect of Different Rates of Nitrogen Starter Dose on Nodulation, Growth, Proximate Composition and Yield of Soybean (Glycine max)
    (Nigerian Institution of Professional Engineers and Scientist, 2022-05-29) Isiaka, K; Lawal, Q.O; Olayinka, B.U; Abdulkareem, K.A; Lawal, M.T; Yusuf, S.Y
    Despite the fact that high nitrogen content in the soil can impede nodule formation in soybean, the need for addition of nitrogen fertilizer as a starter dose to supplement the actions of N-fixing bacteria is a necessity because it restores the drained nutrient and results in high yield. To evaluate the stressful effects of different rates of N fertilizer starter dose, a pot experiment was conducted to evaluate the effect of N fertilizer starter dose on nodulation, growth, proximate composition and yield of soybean. Urea was applied at the rates 0, 50, 75 and 100 kgN/ha at planting using side placement method. The experiment was laid out in Randomized Completely Block Design (RCBD) with three replications. Growth assessment was through taking data on plant height, number of branches, number of leaves and internode length. Also, data on nodulation, yield and yield attributes were taken. Proximate compositions (crude protein, crude fat, crude fiber and ash content) were also determined. It was found that 100 kgN/ha of N-fertilizer improved the total nodulation by 33.95% compared with the control. The yield was greatest with application of 100 kgN/ha compared with other application rates because there was 20% yield increase compared with the control. For the proximate composition, crude protein content was 7% above the control with application of 50 kgN/ha. Furthermore, crude fat content was 37% better than the control with the application of 100 kgN/ha. It was concluded that for profitable soybean production in the study area, the use N fertilizer like ureaat 50 kgN/ha as a starter dose is recommended because of its cheapness and high yield that resulted from its application to the crop. Finally, nitrogen starter dose up to 100kgN/ha did not constitute stress to production of soybean variety under test.
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    Stressful Effect of Different Rates of Nitrogen Starter Dose on Nodulation, Growth, Proximate Composition and Yield of Soybean (Glycine max)
    (NIPES Journal of Science and Technology Research, 2022) Kareem, Isiaka; Lawal, Q.O; Olayinka, B.U; Abdulkareem, K.A.; Lawal, Mujidat Temidayo; Yusuf, Sa'adat Yetunde
    Despite the fact that high nitrogen content in the soil can impede nodule formation in soybean, the need for addition of nitrogen fertilizer as a starter dose to supplement the actions of N-fixing bacteria is a necessity because it restores the drained nutrient and results in high yield. To evaluate the stressful effects of different rates of N fertilizer starter dose, a pot experiment was conducted to evaluate the effect of N fertilizer starter dose on nodulation, growth, proximate composition and yield of soybean. Urea was applied at the rates 0, 50, 75 and 100 kgN/ha at planting using side placement method. The experiment was laid out in Randomized Completely Block Design (RCBD) with three replications. Growth assessment was through taking data on plant height, number of branches, number of leaves and internode length. Also, data on nodulation, yield and yield attributes were taken. Proximate compositions (crude protein, crude fat, crude fiber and ash content) were also determined. It was found that 100 kgN/ha of N-fertilizer improved the total nodulation by 33.95% compared with the control. The yield was greatest with application of 100 kgN/ha compared with other application rates because there was 20% yield increase compared with the control. For the proximate composition, crude protein content was 7% above the control with application of 50 kgN/ha. Furthermore, crude fat content was 37% better than the control with the application of 100 kgN/ha. It was concluded that for profitable soybean production in the study area, the use N fertilizer like urea at 50 kgN/ha as a starter dose is recommended because of its cheapness and high yield that resulted from its application to the crop. Finally, nitrogen starter dose up to 100kgN/ha did not constitute stress to production of soybean variety under test.