Browsing by Author "Olayaki, L.A."

Now showing 1 - 3 of 3
  • Item
    Effects of Melatonin on Estrous Cycle Changes Induced by Ethanolic Extract of Cannabis-sativa in Female Wistar Rats.
    (Society for Experimental Biology of Nigeria, 2019) Oluwasola, A.,; Olayaki, L.A.; Ayinde, T.O.
    This study investigated the effects of melatonin and ethanolic extract of Cannabis-sativa (EECS) on oestrus cycle and reproductive hormones in reproduction of female rats. Twenty female rats were assigned into four groups of five animals each, such that the rats in groups I, II, III and IV received orally 1mL distilled water, 2mg/kg body weight (BW) of EECS, 2mg/kg BW of EECS plus 4mg/kg BW of melatonin and 4mg/kg BW of melatonin, respectively. Vaginal smear was taken daily to determine the Stages of oestrus cycle using light microscope (x400) between 8am-9am. Gonadotropin releasing hormone (GnRH), Luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E), progesterone and prolactin were also determined using standard methods. EECS significantly (p<0.05) lengthened the metestrus and diestrus phases of oestrus cycle. However, it significantly (p<0.05) shortened the proestrus and estrus phases. CS significantly (p<0.05) decreased GnRH, FSH, LH, E, progesterone and prolactin levels respectively. All these effects were ameliorated when combined with melatonin to the level comparable to the control. This study showed the gonadotoxic effects of EECS which could be mediated by endocrine disruption. However, these effects could be ameliorated by melatonin. Since the consumption of CS is increasing globally because of its medical uses leading to its legalization, therefore, consumption of melatonin as supplement is recommended for its users to prevent its gonadotoxic effects.
  • Item
    Effects of Nigella sativa aqueous extract on some haematological parameters, lipid peroxidation and osmotic fragility changes in alloxan induced diabetic rats
    (College of Health Sciences, University of Ilorin, Nigeria, 2010) Ayinla M.T; Soladoye, A.O.; Olayaki, L.A.; Suleiman, S.O.
    This study was designed to investigate the effect of extracts of Nigella sativa on some haematological values, lipid peroxidation and osmotic fragility changes in alloxan-induced diabetic rats. Eighteen albino rats were divided into 3 experimental groups of six rats each; control, diabetic untreated, diabetic treated with aqueous extract of Nigella sativa. At the end of experimental period (Four weeks) animals in all the groups were fasted for 12 hours and blood samples were taken for the determination of red blood cell count (RBC), packed cell volume (PCV), Haemoglobin concentration (Hb). In addition Malondialdehyde (MDA, marker of lipid peroxidation) and erythrocytes osmotic fragility test were carried out. In diabetic untreated rats, the PCV, RBC, Hb. were decreased, but application of the extract significantly increased the values (P<0.05, n=6). Similarly, treatment with aqueous extract of Nigella sativa decreased the elevated Malondialdehyde level (P<0.05, n=6) of diabetic rats. The results also showed that Nigella sativa extract significantly reduced the MCF50 from 0.68% to 0.50% (P<0.05 n=6). These findings suggest that Nigella sativa extract might improve the diabetic-induced disturbances of some haematological parameters by decreasing the elevated lipid peroxides and susceptibility of red blood cell to haemolysis.
  • Item
    Melatonin Mitigates Oxidative stress in Ethanolic Extract of Cannabis-Treated Female Wistar Rats
    (Nigerian Society of Biochemistry and Molecular Biology, 2019) Oluwasola, A.; Ayinde, T.O.; Olayaki, L.A.
    Cannabis sativa (CS) is one of the commonly abused substances by women of childbearing age. About 3.2% females are cannabis smokers in Nigeria, with varied consequences of congenital abnormalities due to interplay between oxidants, oxidants and antioxidants. This study investigated the effects of melatonin and cannabis on oxidative stress in female rats. Twenty female rats were assigned into four groups of five animals each. Groups I, II, III and IV received orally 1ml distilled water, 2mg/kg body weight (bw) of ethanolic extract of C. sativa (EECS), 2mg/kg bw of EECS plus 4mg/kg bw of melatonin and 4mg/kg bw of melatonin, respectively. Serum level of Superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione reductase, total antioxidant capacity (TAC), lactate dehydrogenase and Malondialdehyde (MDA) were assayed using standard methods. CS significantly (p<0.05) decreased catalase, SOD, glutathione peroxidase, glutathione reductase and TAC levels. But it significantly (p<0.05) increased lactate dehydrogenase and MDA levels. All these effects were ameliorated to the level comparable to the control when the extract was administered in combination with melatonin. CS showed alterations of biochemical parameters which was probably mediated by oxidative stress. However, these effects were ameliorated by melatonin. Since the consumption of CS is increasing globally because of its medical uses, thus, consumption of melatonin as supplement may be recommended.