Browsing by Author "Olaifa, Folashade Helen"
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Item Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid.(Agricultural Research Council, ARC-OVU and University of Pretoria, 2012) Olaifa, Folashade Helen; Ayo, Joseph Olusegun; Ambali, Suleiman Folorunsho; Rekwot, Peter IbrahimExperiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (P < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (P > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration.Item Haematological and antioxidant enzyme response to lead toxicity in male wistar rats(University of Peradeniya, 2017) Okediran, B.S; Biobaku, K.T; Olaifa, Folashade Helen; Atata, A.JThe study evaluated the haematological and some antioxidant enzymes response to lead toxicity in male Wistar rats. Twenty male Wistar rats were divided into four groups viz., A, B, C and D. Group A served as the control while groups B, C and D were treated with 200, 300 and 400 ppm of lead (Pb) as lead acetate, respectively. Doses were orally administered in divided doses by intubation to ensure that each rat had the specified doses, after which they have access to water and feed. At the end of two weeks of treatment, blood samples were collected via the median canthus into heparinised tubes for blood lead determination and haematological analysis after which the remaining blood was centrifuged to obtain the plasma for determination of malonydialdehyde, catalase, superoxide dismutase and peroxidase levels. There was a significant increase in blood lead concentrations ranging from 2.15±0.10 μg/dl to 9.21±0.05 μg/dl which was dose dependent while decreases in packed cell volume and the red blood cell counts ranging from 32-53% and 15-52%,respectively. At the highest dose of 400 ppm of lead there was significant decrease in the neutrophils and lymphocytes. There was significant (P<0.05) dose dependent increases in malondialdehyde while the activities of catalase, peroxidase and superoxide dismutase were significantly (P<0.05) reduced. In conclusion, lead disrupts the haematological system leading to generation of free radicals that overwhelm the antioxidant enzymes thus leading to oxidative stress.