Browsing by Author "Ojuade, Fatimoh"

Now showing 1 - 4 of 4
  • Item
    Evaluation of Nutraceutical content and Topical Anti-inflammatory Activities of Turraea vogelii Hook F. Ex Benth (Meliaceae)
    (Arak University of Medical Sciences, 2019) Olumoh-Abdul, Hidayah; Ayanniyi, Rashidat; Ojuade, Fatimoh; Agbana, Remilekun
    Background: Turraea vogelii has been used as traditional medicine for the treatment of ailments including wounds, stomach aches, malaria, infections, filariasis cutaneous, among others and, it serves as source of food. The present study aimed to evaluate the nutraceuticals content and topical anti-inflammatory effect of hydro-methanol twig extract of Turraea vogelii (METV). Method: METV was obtained using cold maceration with water and methanol in ratio 30:70. The concentration of vitamins A, C and E, total phenolic and total flavonoid contents were evaluated by UV spectrophotometric method. Phenol and xylene-induced ear edema was used to evaluate the topical anti-inflammatory effect in mice. Results: The concentration of vitamins A, C and E was found to be 0.113, 9.303, and 0.020 mg in100 g of dried plant materials, respectively. Total phenolic and flavonoid contents were 0.281 mg/g gallic acid equivalent and 0.112 mg/g quercetin equivalent, respectively. Topical application of METV at a dose of 25 and 50 mg/ear in xylene-induced ear edema produced significant inhibition (P<0.05) of 33 and 54% while in phenol-induced ear edema was significantly (P< 0.05) reduced by 44% and 34%. Dexamethasone (0.1mg/ear) produced an inhibition of 83% and 57% in phenol-induced ear edema. Conclusion: Results from this study demonstrated that Turraea vogelii twigs contain flavonoids and phenolic compounds, which may be responsible for the topical anti-inflammatory effect of the plant extract. Keywords: Anti-Inflammatory Effect, Dexamethasone, Phenol, Topical, Turraea Vogelii Extract, Xylene
  • Item
    Evaluation of anti nociceptive and anti inflammatory activities of leaf extract of Turraea vogelli Hook. f. ex. Benth
    (Pakistan Journal of Pharmaceutical Sciences, 2019-01) Ayanniyi, Rashidat; Ojuade, Fatimoh; Olumoh-Abdul, Hidayah; Salawu, Kayode; Olufadi-Ahmed, Haishat; Adebisi-jose, Gbemisola
    Abstract : The leaf extract of Turraea vogelii Hook. f. ex. Benth . is used in ethnomedicine for the management of pain and inflammation. Anti nociceptive activity was determined using acetic acid induced mouse writhing model. The anti inflammatory activity was investigated using in vitro bovine serum albumin (BSA) denat uration assay and BSA induced hind paw edema in rats. The extract (125 500 mg/kg) administered via the oral route produced a significant ( p <0.005) inhibition of acetic acid induced writhes. The percent inhibition of writhes for extract (500 mg/kg) and dicl ofenac (1 0 mg/kg) was 53.3 and 59.5% respectively. The methanol extract (10 6 1.0 µg /mL) inhibited protein denaturation with IC 50 values of ( 1.06 × 10 3 µg/mL and 2.58 × 10 3 µg/mL) for extract and diclofenac respectively. Furthermore, the leaf extract (62. 5 mg/kg) significantly (p<0.05) inhibited BSA induced paw edema in rats. The methanol leaf extract of T. vogelii has anti nociceptive and anti inflammatory activities. These findings justify the use of the plant in traditional medicine for the management o f pain and inflammation.
  • Item
    Evaluation of Anti-nociceptive and Anti-Inflammatory Activities of Leaf Extract of Turraea vogelii Hook. f. ex. Benth.
    (Faculty of Pharmacy, University of Karachi, 2019) Ayanniyi, Rashidat Oluwafunke; Ojuade, Fatimoh; Abdul-Olumoh, Hidayah; Salawu, Kayode; Olufadi-Ahmed, Haishat; Adebesi-Jose, Gbemisola
    The leaf extract of Turraea vogelii Hook. f. ex. Benth . is used in ethnomedicine for the management of pain and inflammation. Anti nociceptive activity was determined using acetic acid induced mouse writhing model. The antiinflammatory activity was investigated using in vitro bovine serum albumin (BSA) denaturation assay and BSA induced hind paw edema in rats. The extract (125 500 mg/kg) administered via the oral route produced a significant ( p <0.005) inhibition of acetic acid induced writhes. The percent inhibition of writhes for extract (500 mg/kg) and dicl ofenac (10mg/kg) was 53.3 and 59.5% respectively. The methanol extract (10 6 1.0 µg /mL) inhibited protein denaturation with IC 50 values of ( 1.06 × 10 3 µg/mL and 2.58 × 10 3 µg/mL) for extract and diclofenac respectively. Furthermore, the leaf extract (62. 5 mg/kg) significantly (p<0.05) inhibited BSA induced paw edema in rats. The methanol leaf extract of T. vogelii has anti nociceptive and anti inflammatory activities. These findings justify the use of the plant in traditional medicine for the management o f pain and inflammation.
  • Item
    The Protective Effect of Croton zambesicus against Carbon Tetrachloride-Induced Renal Toxicity in Rats
    (2019) Ayanniyi, Rashidat Oluwafunke; Olumoh-Abdul, Hidayah; Ojuade, Fatimoh; Abdullahi, Abdulrasheed; Anafi, Sherifat
    Background: The leaf extract of Croton zambesicus (CZ) is used in traditional medicine for the management of various conditions including kidney disease. The purpose of this study was to determine the protective effects of leaf extract of CZ on rat kidney toxicity induced by carbon tetrachloride. Method: Male albino rats were divided into 6 groups of 5 rats and treated for 5 days with aqueous extract (200-400 mg/kg) and n-butanol fraction (20-40 mg/kg) of CZ. Carbon tetrachloride (2 ml/kg of 40% in olive oil) was administered subcutaneously for 3 days and the rats were treated with the extract and fraction for 3 days. Animals were euthanized on day 8 with intraperitoneal injection of ketamine (60 mg/kg) and xylazine (7.5 mg/kg). Blood sample was collected for the analysis of serum electrolytes, urea and creatinine. Kidney tissue was harvested to determine the antioxidant enzyme activity. Results: Carbon tetrachloride produced increased serum sodium ion, chloride ion, urea and creatinine with decreased superoxide, catalase, glutathione, oxidized glutathione and an increase in malondialdehyde concentration. Treatment of rats with the aqueous leaf extract and n-butanol fraction attenuated the toxic effects of carbon tetrachloride on kidney with a significant decline in serum electrolytes, urea, creatinine and a significant increase in the concentration of antioxidant enzymes. Conclusion: Results from this study revealed that the aqueous leaf extract and n-butanol fraction of CZ had protective effect against carbon tetrachloride-induced renal toxicity in rats, thus justifying the consideration of this plant for the management of kidney disease.