Browsing by Author "Ogbole, O.O."

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    Ethnobotanical Survey, Phytochemical Screening, Growth Inhibitory Effects and Cytotoxicity Evaluation of Medicinal Plants used for Cancer Management in Ilorin Metropolis, Nigeria
    (2021) Salawu, K.M.; Ogbole, O.O.; Abiodun, O.O.; Ajaiyeoba, E.O.
    Cancer is a leading cause of death globally and in many local communities, medicinal plants are employed by traditional medicine practitioners (TMP’s) to manage cancer patients. This study was designed to access information on medicinal plants that are locally employed for cancer management in Ilorin metropolis and evaluate growth inhibitory and cytotoxic potentials of selected plants mentioned from the survey.An ethnobotanical survey was conducted among TMP’s in Ilorin metropolis. Plants selected from the survey were collected at Ilorin and authenticated at the Herbarium Unit Department of Plant Biology University of Ilorin before they were air-dried, pulverised and extracted into aqueous-methanol (30:70%). Phytochemical constituents of the plants were determined and thereafter the extracts obtained were subjected to growth inhibitory assays using Sorghum bicolor radicle and Allium cepa root. Cytotoxicity of the plant extracts using brine shrimp lethality and MTT colorimetric assays was evaluated.Fifteen (15) respondents comprising of 9 herb-sellers and 6 herbalist gave their consent to participate in the ethnobotanical study. Nineteen plants belonging to twelve families were mentioned for the management of cancer. The plants were observed to contain secondary metabolites including saponins, tannins, alkaloids, free and combined anthraquinones, flavonoids and terpenoids. Extracts of Detarium microcarpum stem bark and Prosopis africana whole fruit displayed the highest growth inhibitory and cytotoxic effects, with Detarium microcarpum displayed the higher cytotoxicity against Hep-2C cancer cells (IC50 value of 0.08±0.00 μg/mL) compared to cyclophosphamide (2.69±0.25 μg/mL).This study reveals that Detarium microcarpum and Prosopis africana used traditionally in Ilorin for the management of cancer possess growth inhibitory and cytotoxicity effects. These plants might be further explored for anticancer drug discovery.
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    Phytochemical, Antioxidant, Brine Shrimp Lethality and Antiproliferative Analyses of Solanum dasyphyllum schum. & thonn. Leaf and Fruit Extracts (Solanaceae)
    (Savannah Veterinary Journal, 2019) Sodeinde, O.A.; Salawu, K.M.; Ogbole, O.O.; Ajaiyeoba, E.O.
    Introduction: Solanum dasyphyllum is an edible egg plant belonging to Solanaceae family. The fruit and leaf of S. dasyphyllum are traditionally used to relief swellings and oedema. In this study, we investigated the phyto-chemical constituents, DPPH antioxidant activity, brine shrimp lethality and cytotoxicity on human cancer cell lines of the leaf and fruit extracts of S. dasyphyllum. Methods: The leaf and fruit extracts of S. dasyphyllum were subjected to phytochemical screening, in vitro anti-oxidant assay using DPPH, brine shrimp lethality (BSL) bioassay and MTT colorimetric assay using human can-cer cell lines. Results: The leaf and fruit of S. dasyphyllum tested positive for the presence of alkaloids, tannins, saponins and cardiac glycosides. Extract of the leaf (IC50 of 274.6±2.45 and LC50 of 406.0±7.41) and fruit (278.2±2.68 µg/mL and 766.7±9.14 µg/mL) displayed weak DPPH radical scavenging activity and brine shrimp lethality (BSL) com-pared to ascorbic acid (IC50=20.2±0.36 µg/mL) and cyclophosphamide (LC50 of 98.76±0.15 µg/mL) respectively. Compared to the fruits extracts, the leaf extract was found to be more cytotoxic with CC50 of 28.46±1.42, 2.99±0.15 and 23.33±2.7 µg/mL on the human cancer cell lines Rd, Hep-2c and the normal cell line, Vero, re-spectively in the MTT colorimetric assay. Significance: The leaf and fruit extracts of S. dasyphyllum had weak DPPH activity and elicited mild cytotoxi-city in BSL assay. However, the leaf extract of displayed a higher cytotoxicity than the fruit extract on human cancer line used in the study.