Browsing by Author "Njinga N.S."

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    Characterization, anti-inflammatory and antimicrobial potentials of herbal preparation
    (Faculty of Pharmaceutical Sciences, A.B.U Zaria, 2019) Njinga N.S.; Bakare-Odunola, M.T.; Mustapha, K.B.; Yahaya, T.; Shittu A.O.; Bello, H.R.; Abdullahi, S.T.; Usman, S.O.; Ayanniyi, R.O.; Olufadi-Ahmed, H.Y.
    Herbal product (HP) (from Ricinus communis and honey) is used locally for the management of inflammatory and inflammation related disorders including asthma. The current study was carried out to determine the phytochemicals, total phenolic and flavonoid contents, chemical profile, elemental components, anti-inflammatory and antimicrobial activity of the HP used for treatment of asthma. Total phenolic and flavonoid contents were determined by the Folin-Ciocalteus reagent and AlCl3 colorimetric method respectively. The chemo-profiling was done using TLC. Elemental analysis was determined using Atomic Absorption Spectroscopy (AAS). Albumin-induced paw oedema model was used to determine the anti-inflammatory activity while agar well diffusion method was used to determine the antimicrobial activity of the HP at different concentrations. Flavonoids, saponins, alkaloid, cardiac glycoside, steroids and terpenoids were detected in the HP. Total phenolic and flavonoid contents obtained were 10.00 mg/g gallic acid equivalent and 336.60 mg/g quercetin equivalent respectively. The chemo-profiling showed one spot at 254 nm but none at 366 nm. AAS indicated the presence of K, Ca, Fe, Na, Zn, Cu and Pb (Cu and Pb were within WHO acceptable limit). The 100 % concentration produced sudden but transient significant reduction in oedema size at 30 minutes. However, at 60 minutes the effect produced at 25 % was comparable to that of Ibuprofen at 20 mg/kg body weight. At 100% concentration HP all the organisms tested were sensitive except for Pseudomonas aeruginosa. The presence of flavonoids may justify the anti-inflammatory and antimicrobial activities thus validating its use in local treatment of Asthma
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    Evaluating the hypoglycaemic, anti-inflammatory, and antioxidant effects of Hibiscus sabdariffa in alloxan-induced diabetic rats
    (I. Horbachevsky Ternopil National Medical University, Ukraine, 2025) Ojulari L.S.; Njinga N.S.; Ganiyu R.A.; Ayinde T.O.; Kadir E.R.
    Hibiscus sabdariffa is beneficial in treating diabetes mellitus. This study investigated the hypoglycaemic, antiinflammatory, and antioxidant effects of Hibiscus sabdariffa in alloxan-induced diabetic rats. Thirty Wistar rats were divided into six groups of five and acclimatised for two weeks before the experiment commenced. Group I: non-diabetic control; Group II: diabetic control; Group III: non-diabetic with 200 mg/kg of Hibiscus sabdariffa; Group IV: non-diabetes with 300 mg/kg of Hibiscus sabdariffa; Group V: diabetic with 200 mg/kg of Hibiscus sabdariffa; Group VI: diabetic with 300 mg/kg of Hibiscus sabdariffa. The rats received a single intraperitoneal injection of alloxan (150 mg/kg of body weight), and diabetic rats were treated with Hibiscus sabdariffa for 21 days. Fasting blood glucose levels, insulin levels, superoxide dismutase, catalase, malondialdehyde, interleukin-6, and tumour necrosis factor-alpha were measured, and organ and blood samples were collected. The results were analysed using analysis of variance with p < 0.05 considered significant, and data were visualised using GraphPad. This study demonstrated that Hibiscus sabdariffa exerts significant effects on diabetic parameters, pro-inflammatory cytokines, and antioxidant enzymes. Daily oral treatment for 21 days lowered fasting blood glucose, interleukin-6, tumour necrosis factor-alpha, and malondialdehyde levels. It also enhanced insulin production, superoxide di smutase, and catalase activity in the skeletal muscle, liver, pancreas, and kidney. It can be concluded that Hibiscus sabdariffa is beneficial in treating diabetes mellitus. This study investigated the hypoglycaemic, antiinflammatory, and antioxidant effects of Hibiscus sabdariffa in alloxan-induced diabetic rats. Thirty Wistar rats were divided into six groups of five and acclimatised for two weeks before the experiment commenced. Group I: non-diabetic control; Group II: diabetic control; Group III: non-diabetic with 200 mg/kg of Hibiscus sabdariffa; Group IV: non-diabetes with 300 mg/kg of Hibiscus sabdariffa; Group V: diabetic with 200 mg/kg of Hibiscus sabdariffa; Group VI: diabetic with 300 mg/kg of Hibiscus sabdariffa. The rats received a single intraperitoneal injection of alloxan (150 mg/kg of body weight), and diabetic rats were treated with Hibiscus sabdariffa for 21 days. Fasting blood glucose levels, insulin levels, superoxide dismutase, catalase, malondialdehyde, interleukin-6, and tumour necrosis factor-alpha were measured, and organ and blood samples were collected. The results were analysed using analysis of variance with p < 0.05 considered significant, and data were visualised using GraphPad. This study demonstrated that Hibiscus sabdariffa exerts significant effects on diabetic parameters, pro-inflammatory cytokines, and antioxidant enzymes. Daily oral treatment for 21 days lowered fasting blood glucose, interleukin-6, tumour necrosis factor-alpha, and malondialdehyde levels. It also enhanced insulin production, superoxide di smutase, and catalase activity in the skeletal muscle, liver, pancreas, and kidney. It can be concluded that Hibiscus sabdariffa is beneficial in treating diabetes mellitus. This study investigated the hypoglycaemic, antiinflammatory, and antioxidant effects of Hibiscus sabdariffa in alloxan-induced diabetic rats. Thirty Wistar rats were divided into six groups of five and acclimatised for two weeks before the experiment commenced. Group I: non-diabetic control; Group II: diabetic control; Group III: non-diabetic with 200 mg/kg of Hibiscus sabdariffa; Group IV: non-diabetes with 300 mg/kg of Hibiscus sabdariffa; Group V: diabetic with 200 mg/kg of Hibiscus sabdariffa; Group VI: diabetic with 300 mg/kg of Hibiscus sabdariffa. The rats received a single intraperitoneal injection of alloxan (150 mg/kg of body weight), and diabetic rats were treated with Hibiscus sabdariffa for 21 days. Fasting blood glucose levels, insulin levels, superoxide dismutase, catalase, malondialdehyde, interleukin-6, and tumour necrosis factor-alpha were measured, and organ and blood samples were collected. The results were analysed using analysis of variance with p < 0.05 considered significant, and data were visualised using GraphPad. This study demonstrated that Hibiscus sabdariffa exerts significant effects on diabetic parameters, pro-inflammatory cytokines, and antioxidant enzymes. Daily oral treatment for 21 days lowered fasting blood glucose, interleukin-6, tumour necrosis factor-alpha, and malondialdehyde levels. It also enhanced insulin production, superoxide di smutase, and catalase activity in the skeletal muscle, liver, pancreas, and kidney. It can be concluded that Hibiscus sabdariffa is beneficial in treating diabetes mellitus. This study investigated the hypoglycaemic, antiinflammatory, and antioxidant effects of Hibiscus sabdariffa in alloxan-induced diabetic rats. Thirty Wistar rats were divided into six groups of five and acclimatised for two weeks before the experiment commenced. Group I: non-diabetic control; Group II: diabetic control; Group III: non-diabetic with 200 mg/kg of Hibiscus sabdariffa; Group IV: non-diabetes with 300 mg/kg of Hibiscus sabdariffa; Group V: diabetic with 200 mg/kg of Hibiscus sabdariffa; Group VI: diabetic with 300 mg/kg of Hibiscus sabdariffa. The rats received a single intraperitoneal injection of alloxan (150 mg/kg of body weight), and diabetic rats were treated with Hibiscus sabdariffa for 21 days. Fasting blood glucose levels, insulin levels, superoxide dismutase, catalase, malondialdehyde, interleukin-6, and tumour necrosis factor-alpha were measured, and organ and blood samples were collected. The results were analysed using analysis of variance with p < 0.05 considered significant, and data were visualised using GraphPad. This study demonstrated that Hibiscus sabdariffa exerts significant effects on diabetic parameters, pro-inflammatory cytokines, and antioxidant enzymes. Daily oral treatment for 21 days lowered fasting blood glucose, interleukin-6, tumour necrosis factor-alpha, and malondialdehyde levels. It also enhanced insulin production, superoxide di smutase, and catalase activity in the skeletal muscle, liver, pancreas, and kidney. It can be concluded that Hibiscus sabdariffa has the potential to manage hyperglycaemia and inflammation while improving antioxidant enzyme activity. Furthermore, it may serve as a natural source or agent for the treatment or prevention of diabetes