Browsing by Author "Moyosore Salihu Ajao,"

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    Craniofacial genetics as a differential identification tool: analysis of a subset of Yoruba-speaking population in Nigeria
    (Egyptian Journal of Medical Human Genetics, 2025) Suwebat Bidemi Kareem,; Olugbenga Akinola,; Oluyinka Ajibola Iyiola,; Misturah Yetunde Adana,; Ade Stephen Alabi,; Aminu Imam,; Moyosore Salihu Ajao,
    Background Population-dependent genetics and normal-range variations in facial morphology have been reported across several populations, but there is paucity of literature on the African population. The study was designed to evaluate the potentials of craniofacial genetics as a differential identification tool amongst the Yoruba-Ethnic nationality in Nigeria. An anthropometric study of two thousand one hundred and nine (2109) randomly selected individuals (age 15–29 years) was conducted. The personal information of participants was obtained using semi-structured, self-administered questionnaires, while craniofacial parameters were measured from facial photographs with Digimizer software. DNA was isolated from buccal swabs samples obtained from three hundred participants (300) after careful stratifications to match the chosen ethic group. Segments of two craniofacial-associated genetic markers (PAX3 and BMP4 genes) were amplified from participant’s DNA samples using polymerase chain reaction technique. The amplified gene segments were purified, sequenced, and aligned with the reference sequences from the NCBI database. Results A total of 45 samples were observed with synonymous and non-synonymous changes across the 2 genes. These genetic changes were not significantly associated with craniofacial differences in the study population. However, two participants, one each for PAX3 and BMP4, displayed higher polymorphisms that were associated with values of different craniofacial linear parameters {Nasal width (al–al), Nasal height (n-sn), Morphological facial height (n-gn), Bizygomatic distance (zy–zy), Interendocanthal width (en–en), Ear width (t-pa), Ear height (sa-sba), Mandible height (sto-gn), Mouth width (ch–ch), Vermillion height (ls-sto), Eye fissure width@ R(en-ex)}, different from the general population not statistically significant. Conclusion The slight differences in craniofacial parameter measurements in two individuals, with novel polymorphisms (SNPs) loci in PAX3 and BMP4 provides insights to baseline data for validation of cranio-genetic markers that may be useful in the population under study
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    Protective Effect of Nigella Sativa (Black Caraway) Oil on Oral Dichlorvos Induced Hematological, Renal and Nonspecific Immune System Toxicity in Wistar Rats
    (Arak University of Medical Sciences and Iranian Society of Toxicology, 2017-12) Moyosore Salihu Ajao,; Adebayo Babatunde Sansa,; Aminu Imam,; Abdulmumin Ibrahim,; Misturat Yetunde Adana,; Abdulmusawwir Alli-Oluwafuyi,; Suwebat Bidemi Kareem
    Background: Exposure to environmental toxins such as organophosphates poses a great threat to the health of the public. In this work, we investigated the effects of continuous exposure to dichlorvos (DDVP) on kidney function and hematological parameters, and the possible antidote activity of Nigella sativa oil (NSO).Methods: This research was conducted in 2016, at The Animal Holding and Research Laboratory of Faculty Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria. Twenty-four Wistar rats were randomly divided into four groups, six rats each. The four groups received: 1. phosphate buffer solution as controls, 2. DDVP, 3. DDVP+NSO and 4. NSO alone. After 2 wk of treatment, blood samples were collected and hematological profile (RBC, Hb), erythrocyte indices (MCV, MCH, MCHC, and Plt), renal function parameters (albumin, urea, total protein, chloride, sodium, and potassium ions) and nonspecific immune response (WBC) were measured. Results: Rat exposed to DDVP showed red blood cell count, hemoglobin, packed cell volume, albumin, and total protein levels was reduced from control, while white blood cell count and urea significantly increased as compared to controls, the change in K+ level was not significant. NSO maintained optimal levels of red blood cell count, hemoglobin, packed cell volume, albumin, white blood cell count, and urea, indicative of its protective effect against hemo-, immuno- and nephrotoxicity of DDVP.Conclusion: N. sativa (Black Caraway) oil might be a potential antidote in hematotoxicity, immunosuppression and renal dysfunction in organophosphate poisoning, especially dichlorvos. The protective effect of NSO against dichlorvos toxicity can be attributed to its antioxidant capacity.