Browsing by Author "Lawal, B.A."
Now showing 1 - 3 of 3
Results Per Page
Sort Options
Item Phytochemical, Antioxidant and Antimicrobial activities of the Hydroalcoholic Extract of the Roots of Adenodolichos paniculatus against selected Pathogenic Microorganism(Pharmaceutical Society of Kenya, 2022) Njinga, S.N.; Lawal, B.A.; David, S.M.; Eniayewu, I.O.; Bello, N.; Shittu, A.O.; Kolawole, A.J.; Ene, O.C.; Bamidele, O.D.; Saka, K.L.; Kayode, M.S.; Rahimat, O.O.Drugs derived from natural sources present a significant alternative therapy in the management of infectious diseases. The increasing resistance of pathogenic microorganisms to orthodox medicine has necessitated the search for alternative means of treatment. This study aimed at evaluating the phytochemicals, antioxidant and antimicrobial activities of the hydroalcoholic extract of the roots of Adenodolichos paniculatus against selected pathogenic microorganisms. Evaluation of phytochemical constituents was done using standard procedures and the antioxidant activity was evaluated using 2,2-diphenyl-2-pic¬ryl-hydrazyl. Antimicrobial activity was investigated using fifteen pathogenic organisms, which included five gram-positive and seven gram-negative bacterial strains and three fungal strains using Agar diffusion method. The zones of inhibition of the extract against the test organisms, their minimum inhibitory concentrations (MIC) as well as the minimum bactericidal/fungicidal concentrations (MBC/MFC) were determined. The extract was found to contain steroids, flavonoids, alkaloids and saponins. The total phenols, flavonoids, alkaloids and saponins were found to be 1.427±0.02 mg/g gallic acid, 0.4239±0.19 mg/g quercetin, 5% and 75.23% respectively. The antioxidant activity at concentrations of 7.53 and 15.51 μg/mL were not statistically different from that of vitamin C (P<0.05) and the IC50 (Half-maximal inhibitory concentration) found to be 4.906 μg/mL. The zones of inhibition ranged from 22 – 47 mm while the MIC and MBC/MFC was observed to be 5 to 10 mg/mL, and 10 to 40 mg/mL respectively. The availability of phytochemicals with remarkable antioxidant activities and the demonstration of antimicrobial activity against gram-positive, gram-negative bacteria and fungi strains show that A. paniculata root is a potential source for the production of antioxidants as well as antimicrobial drugs.Item Phytochemical, Antioxidant and Antimicrobial activities of the Hydroalcoholic Extract of the Roots of Adenodolichos paniculatus against selected Pathogenic Microorganisms(Pharmaceutical Journal of Kenya, 2022) Njinga, S.N.; Lawal, B.A.; David, S.M.; Eniayewu, I.O.; Bellol, N.; Abiodun, Kolawole, A.J. O.S.; Ene, O.C.; Bamidele, O.D; Saka, K.L.; Kayode, M.S.; Rahimat, O.O.Drugs derived from natural sources present a significant alternative therapy in the management of infectious diseases. The increasing resistance of pathogenic microorganisms to orthodox medicine has necessitated the search for alternative means of treatment. This study aimed at evaluating the phytochemicals, antioxidant and antimicrobial activities of the hydroalcoholic extract of the roots of Adenodolichos paniculatus against selected pathogenic microorganisms. Evaluation of phytochemical constituents was done using standard procedures and the antioxidant activity was evaluated using 2,2-diphenyl-2-picryl-hydrazyl. Antimicrobial activity was investigated using fifteen pathogenic organisms, which included five gram-positive and seven gram-negative bacterial strains and three fungal strains using Agar diffusion method. The zones of inhibition of the extract against the test organisms, their minimum inhibitory concentrations (MIC) as well as the minimum bactericidal/fungicidal concentrations (MBC/ MFC) were determined. The extract was found to contain steroids, flavonoids, alkaloids and saponins. The total phenols, flavonoids, alkaloids and saponins were found to be 1.427±0.02 mg/g gallic acid, 0.4239±0.19 mg/g quercetin, 5% and 75.23% respectively. The antioxidant activity at concentrations of 7.53 and 15.51 µg/mL were not statistically different from that of vitamin C (P<0.05) and the IC50 (Half-maximal inhibitory concentration) found to be 4.906µg/mL. The zones of inhibition ranged from 22 â 47 mm while the MIC and MBC/MFC was observed to be 5 to 10 mg/ mL, and 10 to 40 mg/mL respectively. The availability of phytochemicals with remarkable antioxidant activities and the demonstration of antimicrobial activity against gram-positive, gram-negative bacteria and fungi strains show that A. paniculata root is a potential source for the production of antioxidants as well as antimicrobial drugs.Item Phytochemical, antioxidant and antimicrobial studies of partitioned fractions of Lannea kerstingii Engl. And K. Krause (Anarcadiaceae)(West African College of Postgraduate Pharmacists, 2021) Njinga, N.S.; David, S.M.; Shittu A.O.; Lawal, B.A.; Bello, R.H.; Attah, F.A.U.; Usman, S.O.; Abdullahi, A.A.; Bakare-Odunola, M.T.Background: Antimicrobial resistance is a serious threat to global public health and requires actions like the development of new antimicrobial with significant activities over existing drugs. Objectives: This study aims at investigating the phytochemical, antioxidant and antimicrobial potentials of partitioned fractions of Lannea kerstingii. Methods: A quantitative phytochemistry of chloroform, ethyl acetate, acetone and methanol fractions of L. kerstingii for total flavonoid, phenol and alkaloid were done using standard method; antioxidant and antimicrobial activity were determined using 1,1-Diphenyl-2-picrylhydrazyl and agar diffusion method respectively. Results: The chloroform fraction contained only alkaloid (11%) and steroids while all the other fractions contained phenolic compounds in the range 0.43 to 0.67 mg/g garlic acid. The total flavonoid content ranged from 0.43 to 0.67 mg/g of quercetin. The total flavonoid in the ethyl acetate was significantly different from that of the methanol fraction but not with the acetone fraction. The acetone fraction showed highest antioxidant activity (60.4%) at 0.05 mg/mL though not as comparable to vitamin C. The ethyl acetate showed high antimicrobial activity as it was active against most of the organisms tested upon and zone of inhibition ranged from 13±0.02 to 29±0.1 mm. The acetone fraction was active only against T. mentagrophytes while the methanol fraction showed no activity. Conclusion: The antioxidant and antimicrobial activities may be due to the presence of flavonoids, as well to the presence of tannins and terpenoids present in the different fractions. This makes the ethyl acetate fraction a good source of antioxidant and antimicrobial agent.