Browsing by Author "Lateef, A.A"
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Item Molecular Characterization of Potential Crop Pathogens Associated with Weeds as Endophytes in Uniilorin Plantations, Nigeria(College of Science for Women/ University of Baghdad, 2022-05-20) Lateef, A.A; Garuba, T; Abdulkareem, K.A; Olayinka, B.U; Olahan, G.S; Adeyemi, S.B; Abdulrahaman, A.ACrop diseases are usually caused by inoculum of pathogens which might exist on alternate hosts or weeds as endophytes. These endophytes, cum pathogens, usually confer some beneficial attributes to these weeds or alternate hosts from protection against herbivores, disease resistance, stress tolerance to secondary metabolites production. This study was therefore carried out to isolate potential crop pathogens which exist as endophytes on weed species in the University of Ilorin plantations. Green asymptomatic leaves were collected from 10 weed species across the plantations, and processed for their endophytic fungi isolation. Isolates were purified into pure cultures and used for molecular identification using the internal transcribed spacer (ITS) region of the ribosomal DNA. Phylogenetic analysis of the fungal sequences using MEGA software revealed 9 fungal genera belonging to 13 species, with species in the genera Curvularia, Epicoccumand Daldinia occurring in more than one weed species, while other genera such as Alternaria, Fusarium, Chaetomium, Macrophomina, Arthrinium and Phomopsis occurred in just one weed species each. Daldinia eschscholtzii was isolated in this study as an endophyte from Loudetia arundinacea for the first time. This plant is very abundant in Nigeria and Africa where it is used majorly for thatching and feeding livestocks. This also represents the first endophytic fungi from the genus Loudetia. Potential relationship between the occurrences of these fungi as endophytes and as pathogens are discussed. These discoveries represent the first large-scale molecular identification and several first reports of endophytes from these weed species. These results also represent the first records of some of these fungi in Nigeria.Item Phylogenetic Position of Nigerian Species of Curcuma longa (Zingiberaceae) in the Current Infrageneric Classification(The College of Natural and Applied Sciences, Fountain University, Osogbo, Nigeria, 2024) Tiamiyu, B.B; Lateef, A.A; Sagaya, A; Abdulkareem, K.A; Olayinka, B.U; Ayinla, A; Adeyemi, S.B; Amenu, S.G; Abdulrahaman, A.A; Mustapha, O.TCurcuma longa L. (commonly known as Tumeric) is the only species of the genus Curcuma found in Nigeria. It is of great economic importance to Nigeria, Africa, Asia, and other parts of the world, where it is widely used for ornamental and medicinal purposes, and as spices in food and beverages. However, the phylogenetic placement of the turmeric plant (C. longa) in Nigeria is far from being fully resolved, hence the need for this study. The rhizomes of turmeric were collected at the Forestry Research Institute of Nigeria, Ibadan, Oyo state. Genomic DNA was extracted, followed by the amplification of the ITS and psbA-trnH regions. Phylogenetic analysis was conducted using the Maximum likelihood method. The result resolved the phylogenetic position of Nigerian species and supported existing subgenera classification into three clades, all with high bootstrap support for the three clades. The result of this study supports the subgenera classification of the genus and further reveals the phylogenetic position of C. longa.