Browsing by Author "Krishnamurthy, Ramar"

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    ANTIBACTERIAL ACTIVITY OF URENA LOBATA AGAINST UROPATHOGENS
    (Nigerian Society of Pharmacognosy, 2021) Garuba, Taofeeq; Katrodiya, Nency; Patel, Nikital; Patel, Swetal; Rajani Dhanji P.; Chettiar, Shiva S.; Krishnamurthy, Ramar
    Urinary tract infections (UTI) are one of the most common form of bacterial infections but the treatment becomes cumbersome as the etiological bacteria are developing resistance against antibiotics. This present study evaluated the efficacy of antimicrobial activity of Urena lobata against uropathogens. Six urine samples from UTI patients were collected from Pathological Laboratory, G.B. Vaghani Multispecialty Hospital, Surat. Bacteria were isolated from these samples using Nutrient agar, Mac Conkey agar plate, Blood agar, Mannitol salt agar, Eosin Methylene Blue agar and King’s agar. The bacterial isolates were identified using cultural characteristics, microscopic features and biochemical characteristics. Leaf extract of Urena lobata was prepared using Soxhlet Extraction Method whereby methanol and distilled water were the extractants used. Herbal extract disc was prepared at concentrations of 50,75, and100 mg/ml and tested against all the isolates. DMSO and antibiotics (Nitrofurantion, Amikacin, Levofloxacin, Norofloxacin, Ofloxacin and Cephalosporins) were used as negative and positive controls respectively.Staphylococcus aureus, Escherichia coli, Bacillus cereus, Streptococcus pneumoniae, Klebsiella spp. and Brevibacillus panacihumi were isolated from the urine samples. All concentrations of aqueous and methanolic extracts of U. lobata leaf displayed highest zone of inhibition against B. cereus. No inhibitory effect was observed against the growth of Klebsiella except at the highest concentrations. Further study is encouraged on the in-vivo study of efficacy of U. lobata on etiological agent of UTI.
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    MORPHOLOGY, GROWTH VARIABILITY AND CHEMICAL COMPOSITION OF INDIAN AND NIGERIAN ACCESSION OF OCIMUM SPECIES GROWN IN INDIA
    (Chemistry-Biology Department of North University of Baia Mare Romania., 2019) Patel, Nensi; Patel, Nikita; Patel, Swetal; Ingalhalli, Rajashekhar; Garuba, Taofeeq; Ahmed, Akeem O.; Oyeyinka, Samson A.; Krishnamurthy, Ramar
    Demand for medicinal plants is increasing by the day, due to their health benefits. In this study, the morphological attributes, proximate, mineral and phytochemical compositions of Indian accessions of Ocimum sanctum and Ocimum gratissimum as well as that of Ocimum gratissimum (Nigerian accession) grown in India under greenhouse conditions were investigated. Ocimum leaves showed significant variations in their morphometric attributes and these attributes increased with increasing days after planting. The leaves were good sources of fibre and relatively low in protein. O. sanctum showed superior iron content than the other species. Total phenolic and cardiac glycoside contents of the three leaves were very similar but they showed significant variations in their saponins, tannins, flavonoids and alkaloid contents. PCA revealed that Indian accessions of O. sanctum and O. gratissimum are distinctly separated and different from Nigerian accession of O. gratissimum in morphometric data and phytochemical constituents. Nigerian accession had superior phytochemical contents than the Indian accessions and may be further explored for breeding purposes to complement the Indian accessions for enhanced applications in the pharmaceutical industry in India.
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    Secondary metabolites constituents and antibacterial screening of methanolic leaves extract of Senna fistula and Ocimum gratissimum.
    (2019-01) Ayinla, Maryam Tayo; Adeyemi, Sherif B; Owoyele, B.V.; Krishnamurthy, Ramar