Browsing by Author "Krishnamurthy, R"

Now showing 1 - 3 of 3
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    Evaluation of genetic variation among populations of Dipcadi filamentosum Medik in some geographical regions in Nigeria based on RAPD markers
    (University of Peradeniya, Sri Lanka, 2018-05-09) Abdulkareem, K.A; Mustapha, O.T; Krishnamurthy, R
    The genetic variations were studied in different populations of Dipcadi filamentosum Medik collected from various geographical locations in Nigeria using 12 random amplified polymorphic DNA (RAPD) markers. Genomic DNA extraction was carried out using DNeasy Plant Mini Kit (QIAGEN, USA) and amplification of fragments was performed by Polymerase Chain Reaction. Amplification by 9 primers resulted in the detection of 95 loci and this represented 100% polymorphism. The sizes of the bands ranged between 1500-50bp in OPAE-14 and OPAC 11.The Principal Component Analysis (PCA) showed that the genetic variation observed were accounted for by the first three components (81.14%). Biplot analysis indicated that the markers effectively separated the populations into groups based on genetic similarity. The cluster analysis classified the populations into two major clusters with nine groups. The Neighbour joining clustered populations from YOB (north east,), OYO (south west) and KBA (north central) as genetically related and close neighbours.
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    Hazard assessment and cytogenotoxic effect of different concentrations of mercury chloride sterilant using the Allium cepa assay
    (2024) Animasaun, D.A.; Adedibu, P.A.; Afolabi, S.O.,; Abdulkareem, K.A.; Ibrahim, S.; Krishnamurthy, R
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    Secondary Metabolites constituents and Antibacterial Screening of Methanolic Leaves Extracts of Senna fistula and Ocimum gratissimum.
    (College of Health Sciences, University of Ilorin, Nigeria, 2019) Ayinla M.T; Adeyemi, S.B; Owoyele, B.V.; Krishnamurthy, R
    The misuse of antibiotics has contributed largely to the development of resistance strains thereby creating the need for an incessant search and development of newer drugs. This study investigated the secondary metabolites constituents and antibacterial activity of methanolic leaves extracts of Senna fistula (SF) and Ocimum gratissimum (OG). The SF and OG leaves extracts were done using methanol, the phytochemical analysis were done as per standard protocol. The antibacterial activity was determined by agar well diffusion method. The results showed that the methanolic leaves extracts of SF and OG contain secondary metabolites like flavonoids, tannins, alkaloids, terpenoids, cardiac gylcosides, saponins, phenolic compounds. The antibacterial screening revealed that both Senna fistula and Ocimum gratissimum exhibited strong inhibitory activity against all the tested organisms. Methanolic leaf extract of SF has a stronger antibacterial activity with zones of inhibition of 19.5mm against E coli and 31.5 mm against Klebsiella than methanolic leaf extract of OG (18 mm against E coli and 28 mm against Klebsiella) in a dose dependent manner. In conclusion, the methanolic leaves extracts of Senna fistula and Ocimum gratissimum exhibited antibacterial activity which is due to the presence of various secondary metabolites; therefore this study supports its use as an antibiotic agent.