Browsing by Author "Kazeem, Muinat Olanike"

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    Genome sequencing of endophytic bacterial species associated with Datura stramonium and Sida acuta
    (Zagazig University, 2025) Zakariyah, Rahmat Folashade; Ajijolakewu, Kamoldeen Abiodun; Ayoola, Saheed Abiodun; Ajadi, Ayobami Elias; Kazeem, Muinat Olanike; Oyedele, Julius Olusegun
    Background: Endophytes are ubiquitous microbes that colonize plants’ tissues without causing any harm to the host plants, but rather, they confer several adaptable characteristics on them. They could be of bacterial, fungal, algal, archaeal and actinomycetes origins. Endophytes also produce useful bioactive metabolites some of which are potent antimicrobials. In this study, bacterial endophytes were isolated from the leaves of Datura stramonium and Sida acuta, both of which are important medicinal plants. Methods: Freshly cut leaves were collected, rinsed and sequentially sterilized. The sterilized leaves were then cut into smaller pieces and incubated onto extract-seeded nutrient agar medium augmented with antifungal drug. Pure distinct bacterial colonies were morphologically and biochemically characterized. Molecular characterization of the strains was done using the 16S rRNA while phylogenetic evolutionary analysis was carried out using the maximum likelihood method and Tamura-Nei model with MEGA 11 software. Results: A total of 7 bacterial endophytes were isolated and identified, 3 from D. stramonium and 4 from S. acuta. 16S rDNA genome sequencing of the strains revealed that isolates from D. stramonium were Pseudomonas aeruginosa, Atlantibacter hermannii and Enterobacter roggenkampii while those isolated from S. acuta were identified as Pseudomonas aeruginosa, Pseudomonas monteilii, Enterobacter pseudoroggenkampii and Aeromonas veronii. Conclusion: The outcome of this research has indicated that numerous strains of endophytic bacteria inhabit leaves of plants. It is therefore essential to exploit these endophytic bacteria for their potentials to produce bioactive metabolites and other valuable antimicrobial products.
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    Optimizing Laccase Production from Delonix regia Pods by Aspergillus carbonarius F5 Using Response Surface Methodology and it's Dye Decolorization Potential
    (Science domain international, 2022-06) Kazeem, Muinat Olanike; Arekemase, Musa Olusegun; Tiamiyu, Aishat Omolewa; Ugba, Mhii
    Introduction: Laccase is a muilti-copper enzyme that directs oxidation of compounds by reducing oxygen to water molecules. It has been identified as a significant enzyme which possesses diverse interest in industrial sector due to its ability to degrade toxicants from dye-utilizing related industries. Aim: The current research focuses on the optimization of laccase from Aspergillus carbonarius F5 on Delonix regia pods, for application in dye degradation. Methodology: Potential laccase producing fungi were screened and characterized using 18s rRNA. Laccase production was further conducted on untreated and acid pretreated Delonix regia pods. Plackett-Burman design and Central Composite Design (CCD) of the Response Surface Methodology (RSM) was used to screen for various nutritional and environmental factors to improve the yield of the enzyme and then applied for dye decolorization studies. Results: The fungal strain with the highest laccase activity was identified as Aspergillus carbonarius F5. The acid pretreated Delonix regia pods showed considerably high enzyme activity when compared with untreated. Moreover, variation of notable nutritional and environmental factors were detected to improve laccase yield. The effect of input parameters such as incubation period, pH, temperature and MgSO4 were documented as the major factors influencing high laccase yield using Plackett-Burman design. Results obtained from the modelling of experiment using CCD-RSM shows that maximum laccase production of 8.04 U/ml was recorded at temperature 36°C, pH 6, MgSO4.7H2O at 0.2 (g/L), and 7 days which reveals a 8.28 –fold increase. The outcome of the investigation performed on dye decolorization suggests an 87.6 % and 62.6 % degradation on congo red and malachite green dyes respectively. Conclusion: Delonix regia pods could be an added substrate, suitable for optimization of laccase by Aspergillus carbonarius F5 and the enzyme was capable of decolorizing industrial dyes.