Browsing by Author "Kazeem, M.O"
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Item Basic Laboratory Practicals in Microbiology(Department of Microbiology, University of Ilorin, Nigeria., 2017-05-12) Olayemi, A.B.,; Sani, A.,; Oyeyiola, G.P; Omojasola, P.F.; Kolawole, O.M.; Arekemase, M.O; Ahmed, R.N.; Anibijuwon, I.I.; Sule, I.O; Agbabiaka, T.O.; Adebisi, O.O; Udeze, A.O; Ahmed El-Imam, A.M; Oke, M.A; Kazeem, M.O; et alItem Bioconversion of Aspergillus niger KM treated rice and wheat bran for experimental rat feed formulation(Biokemistri., 2024-02-27) Sulaiman, F. A; Kazeem, M.O; Akanji, M; Sulaiman, A.; Igwe, O.; Asunmogejo, R; Rahman, N. AThis study investigated the nutritional value of pre-treated rice and wheat bran wastes as feed formulation in experimental rats. Aspergillus niger KM isolated from decomposing organic matter was tested for cellulase assay. The lignocellulosic agricultural residues (wheat bran and rice bran) were pretreated with ammonia and diluted sulfuric acid after which solid substrate fermentation with Aspergillus niger KM was carried out. Determination of reducing sugar was carried out and the fermented residues were included as components in feed formulation and were fed to different groups of rats for four weeks. The proximate analysis of the feed formulation showed that the NH3 pretreated feeds gave higher protein content of 21.94%, relative to the control or other groups. Growth performances of animals fed with NH3 pretreated wheat bran significantly increased from 158.25 to 201.66 g throughout the feeding periods. Evaluation of the various feeds’ effect on tissue marker enzymes revealed inconsistent alterations relative to the control. Bioconverted wheat or rice bran has nutritive value to support animal growth and could be explored in animal feed preparationItem TAMARIND EXTRACT PRETREATMENT: VALORIZATION OF SUGARCANE BAGASSE FOR CELLULASE PRODUCTION BY Aspergillus flavus(Faculty of science, Obafemi Awolowo University, Ile- Ife, 2021-05-15) Kazeem, M.O; Ajijolakewu, K. A.; Ahmed El - Imam, A M.; Salau, R. B.Effective pretreatment is crucial for cellulase production from sugarcane bagasse. Pretreatment with tamarind extract could reduce the hazardous effect associated with chemical pretreatment. The present work investigated tamarind (Tamarindus indica) extract in combination with H SO and thermal pretreatment of sugarcane bagasse 2 4 for cellulase production by Aspergillus flavus. The sugarcane bagasse was pretreated with tamarind extract pH 2 and pH 4, followed by 1% H SO and thermal treatment at 121°C for 15 min. The pretreatment slurry was 2 4 analysed for reducing sugar while solid bagasse was analysed for weight loss. Aspergillus flavus grew on sugarcane bagasse under solid state fermentation and the Carboxy Methyl Cellulase (CMCase) and Filter Paper Assay (FPA) activities were compared on the various pretreatments. The pretreatments changed the visible morphology of the sugarcane bagasse observed by the swelling, fibrous appearance and colour change. Pretreatment slurry yielded highest soluble reducing sugar at 60.01 mg/ml in tamarind extract (pH 4/1% H SO ) and highest weight 2 4 loss of solids at 73.70% in tamarind extract (pH 2/1% H SO /thermal 121°C). Aspergillus flavus performed better 2 4 on tamarind extract (pH 2/1% H SO ) by producing optimal CMCase and FPA activities at 0.100 U/ml and 2 4 0.409 U/ml respectively after 3 days of fermentation. Cellulase was maximally active at temperature of 50 °C. The tamarind extract pretreatment successfully proved to be an alternative organo-chemical pretreatment of sugarcane bagasse as evidenced by the physical properties, soluble reducing sugars and cellulase activities