Browsing by Author "Kareem, I"
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Item DNA Barcoding of Vernonia amygdalina using ITS and RPOC 1 Multi Loci Gene Regions(Faculty of Science, Federal University Birnin Kebbi, 2023-12) Abdulkareem, K.A; Elebiyo, P.T; Olayinka, B.U; Tiamiyu, B.B; Kareem, I; Danzaki, M.M; Mustapha, O.TVernonia amygdalina is one of the most well-known plants found in Africa and Asia and the most cultivated species of the genus Vernonia. The knowledge of how living and extinct species are related to one another supports much of evolutionary Biology. Therefore, this study was aimed at carrying out the molecular identification of Vernonia amygdalina using ITS and RPOC1 regions. In this study, the identification and phylogenetic analysis of Vernonia amygdalina was done through amplification of the DNA using ITS and RPOC1 primers. The ITS and RPOC1 sequences were compared with the existing sequences in the NCBI GenBank using BLAST searches then the sequences were aligned and maximum likelihood and parsimony trees were constructed using MEGA11. The result showed that ITS sequence length is 627bp while the RPOC1 sequence is 422bp. The blast result for ITS primer sequence showed 93% - 96% similarity against 6 accessions, 70% - 74% against 8 accessions, 64% - 69% against 6 accessions, and 82% similarity against 1 accession. Whereas the RPOC1 primer sequence gave 96% - 99% similarity against all accessions in the BLAST results. Vernonia amygdalina, Hirpicium diffusum, Cyanthillium cinereum, Sonchus ustulatus, Olearia odorata, and Olearia laxiflora were observed as out groups for their respective trees with the RPOC1 primer producing the highest percentage with all BLAST matches ranging from 96% to 99%. This indicates that the RPOC1 primer produced higher identification to species level.Item Effect of salinity stress on growth, yield and nutritional qualities of two okra varieties(Agricultural Academy of Bulgaria, 2020) Kareem, I; Dauda, Z.I; Kareem, S.A; Abdulmaliq, S.Y; Adekola, O.F; Abdulkareem, K.A; Olayinka, B.U; Abdulaziz, A; Alasinrin, S.K; Usman, M; Mahamoud, A,RThe objective of this study was to determine the effect of salt stress on the growth yield and nutri tional quality of two okra varieties. The experiment was laid out in randomized complete block design (RCBD) with three replications. Clemenson spineless NHA-e varieties of okra were the two varieties tested. The treatments used comprised four salinity levels (0 mM, 25 mM, 50 mM, and 75 mM) us ing sodium chloride as the salinity source. The varieties were assessed using plant height, number of leaves, leaf area, days to first flowering and fruit weight. Furthermore, moisture, carbohydrate, crude protein, crude fat, ash and crude fiber contents were used to assess the fruit nutritional qualities. The results showed that Clemenson spineless variety had better tolerance to salinity stress in plant height, days to flowering, fruit mass, ash content and crude protein content. However, NHA-e variety was more tolerant in fruit dry matter, crude fibre content, carbohydrate content and ether extract content. It is concluded that Clemenson spineless was more tolerant to salinity stress than NHA-e variety. Therefore, Clemenson spineless variety should be used in saline areas of Ilorin which is the northern guinea savannah zone of Nigeria and places with the same edaphic and climatic attributesItem EVALUATION OF CALCIUM CARBIDE'S CYTOTOXIC EFFECTS ON ONION (Allium cepa)(Federal University Dutsinma, Nigeria., 2023-06-02) Abdulkareem, K.A; Olayinka, B.U; Sulyman, R.A; Kareem, I; Danzaki, M.M; Mustapha, O.TThe purpose of this investigation was to see if Calcium Carbide (CaC2) had any harmful effects on onions (Allium cepa L.). Allium cepa root tips were grown in various concentrations of Calcium Carbide (CaC2) (0.25g, 0.50g, 0.75g, and 1.00g)/ 250ml, with distilled water serving as a control. For cytological tests, the root tips of Allium cepa growing in the treatments and control were removed daily between 7:30am and 8:30am. Pretreatment, fixation, hydrolysis, squashing, and staining of cells for mitotic investigations were performed, and data on cytological parameters were collected using a light microscope at a magnification of X40. The mitotic index (MI) and karyotype analysis were used to assess the data collected on these parameters. The results of this study showed that root tips treated with 0.25g of Calcium Carbide (CaC2) have a mitotic index of 45.83 and the rate of cell division decreases with an increase in the concentration of Calcium Carbide (CaC2) as the cell divides the mitotic index dropped sharply. These finding indicated that Calcium Carbide is a strong mitotic inhibitor and could give rise to mitotic abnormalities with increase in concentration and also reduced cell division. We therefore recommended that there is need for further investigation using lower concentrations of Calcium Carbide as well as other mutagenic substances in order to ascertain their effect on the chromosomal behaviorItem GENETIC VARIABILITY VIA PROTEIN ELECTROPHORESIS AMONG SOME NIGERIAN ACCESSIONS OF PIGEON PEA (CAJANUS CAJAN)(The Institute of Biology, Bucharest, Romania, 2021) Abdulkareem, K.A; Olayinka, B.U; Danzaki, M.M; Idris, R; Kareem, I; Aziz, A; Sagaya, Q; Mustapha, O.TPurpose: Lack of genetic variability is a limitation to pigeon pea improvement. Hence the need to study the genetic variability of five accessions of pigeon pea with the view of isolating those that are with high yield and early maturity. Research Method: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) was used in the protein extraction and genetic diversity was estimated using Paleontological Statistics (PAST). Findings: A total of 24 protein bands were obtained ranging from 10kDa to 170 KDa. The protein band with the highest molecular weight was generated in accession NG/AO/MAY/09/021, NG/AO/11/08/108, NG/SA/07/0180 and NG/SA/07/208 while lowest molecular weight i.e 10 KDa was generated in NG/AO/MAY/09/021. Highest similarity index was recorded (45.16%) in NG/SA/07/0180 and NG/SA/07/208 and lowest (13.04%) in NG/SA/07/0180 and NG/AO/MAY/09/021. The bands showed variability based on intensity and presence or absence of any of them among the accessions. Jaccard’s similarity separated the 5 accessions into two clusters at an UPGMA similarity coefficient range of 0.2 to 1. Originality/Value: Accession NG/AO/MAY/09/021 occupies a distinct position hence could be combined with other accessions in a breeding programme.Item INFLUENCE OF SEED PRIMING ON YIELD, MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERISTICS OF WATER-DEFICIT STRESSED LOWLAND RICE(West University of Timisoara, Romania, 2021-08-28) Kareem, I; Ismail, M.R; Puteh, A.B; Kareem, S.A; Lawal, O.I; Adekola, O.F; Faseyi, A.F; Olaniyan, J.O; Yusuf, S.Y; Lawal, M.T; Abdulkareem, K.A; Olayinka, B.U; Abdulaziz, A.; Ahmed, O; Baba, H.S; Kuranga, H.A; Lawal, Q.O; Musa, A.I; Oyelade, H.YOne of the potential ways of increasing rice production to cater for ever increasing population of Malaysia is to extend the production area of lowland rice through its production as upland rice. Therefore, this research was conducted to determine the effects of seed priming in alleviating water deficit stress in lowland rice produced as upland rice. The treatments used were 100mM calcium chloride dihydrate for duration of 48hours and temperature of 25oC, 40% w/v polyethylene glycol (PEG) 6000 for a duration of 48hours and temperature of 25oC, 100ppm kinetin for a duration of 24hours and temperature of 4oC, 200ppm methyl jasmonate for a duration of 24hours and temperature of 4oC, stressed control (unprimed seeds) and the unstressed control (unprimed seeds with adequate irrigation). The experiment was laid out in randomized complete block design (RCBD) with three replications. Data were collected on classical growth parameters, germination pattern, gas exchange characteristics, yield and yield components. It was found that priming MR219 rice with PEG could enable the variety to be produced as upland rice with little yield reduction because PEG priming was the best in individual seed mass, final yield, harvest index and water use efficiency. It is, therefore, concluded that 48hours of priming with 40%w/v polyethylene glycol at 25oC be used for MR219 rice seeds whenever MR219 rice variety is to be cultivated as upland rice.Item NPK partitioning, growth, yield and proximate composition of okra (Abelmoschus esculentus) under water deficit stress(Agricultural Academy of Bulgaria, 2020-04) Kareem, I; Faseyi, A.F; Saliu, A.K; Abdulmaliq, S.Y; Adekola, O.F; Abdulkareem, K.A; Olayinka, B.U; Abdulaziz, A.; Alasinrin, S.K; Hakeem, A.K; Usman, M.; Lawal, M.T; Ahmed, O; Lawal, O.I; Bello, W.B; Salami, T.B; Ayeleke, D.A; Azeez, K.O; Olaniyan, J.O; Affinnih, K.O; Olalekan, K.KThis experiment was conducted to determine the effects of drought stress on growth yield, NPK partitioning and nutritional composition of okra. To achieve this objective, four levels of water deficit stress (daily (control), a day interval, two days interval and three days interval irrigation) were tested on 17Lucky19 (hybrid) okra variety in a pot experiment. The experiment was laid out randomized complete block design (RCBD) with three replications. Parameters used to determine the effects of water deficit stress were nitrogen, phosphorus and potassium contents of stems and leaves. Plant height, number of branches, number of leave, number of fruits, fresh fruit mass, dry straw mass, chlorophyll content, and proximate parameters (crude fat, crude fibre, crude protein and ash contents of the leaves) were also used. It was found that all the growth and yield parameters tested reduced with increase in water deficit levels. Similarly, all proximate parameters decreased with increase in water deficit levels with the exception of crude fibre which increased with increase in water deficit levels. In the same vein, nitrogen and potassium levels decreased with increase in water deficit levels in both leaves and stems. However, phosphorus levels in stems and leaves decreased with increase in water deficit levels. It is, therefore, concluded that 17Lucky19 is susceptible to water deficit stress. This implies that water deficit tolerant or resistant varieties should be used instead of 17Lucky19 whenever areas with irregular rainfall are to be used for cultivating this variety of okra.Item Optimization of priming duration for rice production under drought stress(Agricultural Academy of Bulgaria, 2020-04) Kareem, I; Kareem, S.A; Abdulmaliq, S.Y; Adekola, O.F; Abdulkareem, K.A; Olayinka, B.U; Abdulaziz, A.; Yusuf, A.S; Kuranga, A.B; Magaji, U.; Lawal, M.T; Ahmed, O; Lawal, O.I; Bello, W.B; Salami, T.B; Ayeleke, D.A; Azeez, K.O; Olaniyan J,O; Affinnih, K.O; Olalekan, K.KThe type of priming chemical and the duration of priming are important factors determining the performance of plants resulting from priming treatment. Therefore, this research was conducted to determine the effects of different priming agents and soaking durations on growth and yield of drought-stressed rice. To achieve this objective, a pot experiment was conducted where three priming agents (100 mM calcium chloride dihidrate (CaCl2), 40% (w/v) polyethylene glycol (PEG) 6000 and 100 ppm kinetin) combined with two soaking (priming) durations were tested on drought stressed rice plants in a 3x2 factorial experiment. The experiment was laid out in a completely randomized design (CRD) with three replications. Parameters on germination percentage, number of tillers, number of productive tillers, tiller effi ciecy, shoot fresh and dry weight, yield, seed length to seed width ratio (seed size), 100-seed weight and harvest index were taken. Also, data on photosynthetic rate, stomatal conductance, intercellular carbon-dioxide and transpiration rate and leaf water were taken. It was found that the highest yield was got when PEG was used for priming for 48 hours. It was found that number of tillers, shoot dry mass, 100-seed mass, rate of photosynthesis, stomatal conductance, transpiration and leaf area water were increased when seeds were primed for 48 hours with kinetin solution. However, tiller efficiency, harvest index and seed size were favoured by 24-hour priming with PEG while number of productive tillers, shoot fresh mass and grain yield were favoured by 48 hour priming with PEG. It was concluded that 48 hour priming with PEG was effective alleviate moisture stress in MR219 rice. This implies that to avoid wastage of priming chemicals and circumvent undue prolongation of priming period or duration which will result in harming the seeds (toxicity) and poor performance of the resulting plants, 40% (w/v) PEG 6000 should be used for 48 hours for priming MR219.