Browsing by Author "Kadir, R. E."
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Item Anticancer effects of Morinda lucida and Annona muricata on immunomodulations of Melatonin, tumor necrosis factor-alpha and p53 concentrations in lead acetate-induced toxicity in rats(Qassim University, 2021-07) Akinlolu, A. A.; Ameen, M. O.; Oyewopo, A. O.; Kadir, R. E.; Ahialaka, O.; Tijani, S.; Ogungbesan, O.; Bebeyi, R.; Adebayo, S.; Amoo, T.; Abdulazeez, M.Objectives: Lead poisoning accounts for about 0.6% of global burden of disease. Lead-induced toxicity is through confinement of oxidative stress in affected organs. We evaluated the effects of MLF1 (extracted from Morinda lucida leaves) and AMF1 (extracted from Annona muricata leaves) on lipid peroxidation and immunomodulations of Melatonin, tumor necrosis factor-alpha (TNF-α), and p53 proteins in lead acetate (LA)-induced toxicity in rats. Methods: Sixty adult female rats were randomly divided into 12 groups (n = 5). Groups 1 and 2 received physiological saline and 100 mg/kg bodyweight of LA, respectively, for 5 weeks. Groups 3–6 received 100 mg/kg bodyweight LA for 2 weeks, followed by treatments with 7.5 and 15 mg/kg bodyweight of MLF1, and 7.5 and 10 mg/kg bodyweight of AMF1, respectively, for 3 weeks. Groups 7–10 received 7.5 and 15 mg/kg bodyweight of MLF1, 7.5 and 10 mg/kg bodyweight of AMF1, respectively, for 5 weeks. Groups 11–12 received co-administrations of 100 mg/kg bodyweight LA with 15 mg/kg bodyweight MLF1 and 10 mg/kg bodyweight of AMF1, respectively, for 5 weeks. Drugs and extracts were administered orally. Consequently, liver histopathology (Hematoxylin and Eosin), sera Melatonin, and TNF-α (enzyme-linked immunosorbent assay [ELISA]) levels were evaluated. Malondialdehyde (MDA) (thiobarbituric acid assay) and p53 (ELISA) levels were evaluated in liver homogenates. Data were statistically analyzed (P ≤ 0.05). Results: Results showed normal liver histology in all Groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased levels (P ≥ 0.05) of MDA, TNF-α and p53 in Groups 3-12, compared with Group 2. Furthermore, results showed significant (P ≤ 0.05) and non-significant increased Melatonin levels (P ≥ 0.05) in Groups 4–12 compared with Group 2. Conclusion: This study confirmed that MLF1 and AMF1 confer a degree of antioxidant, anticancer and hepato-protetive potentials against LA-induced toxicity in rats.Item Moringa oleifera and Musa sapientum ameliorated 7,12-Dimethylbenz[a]anthracene-induced upregulations of Ki67 and multidrug resistance 1 genes in rats(Qassim University, 2021-05) Akinlolu, A. A.; Oyewopo, A. O.; Kadir, R. E.; Lawal, A.; Ademiloye, J.; Jubril, A.; Ameen, M. O.; Ebito, G. E.Objectives: Moringa oleifera (MO) and Musa sapientum (MS) are plants of ethnomedicinal importance. We evaluated the effects of MOF6 (extracted from MO leaves) and MSF1 (extracted from MS suckers) on immunomodulations of Ki67 (proliferation biomarker) and multidrug resistance 1 (MDR1) genes in the liver of rats in 7,12-Dimethylbenz[a]anthracene (DMBA)-induced hepatotoxicity and mutagenesis to determine their antiproliferation, anti-drug resistance, and anticancer potentials. Methods: Forty-five adult male rats were randomly divided into nine groups (n = 5). Groups 1 and 2 received physiological saline and 15 mg/kg bodyweight of DMBA, respectively. Groups 3 and 4 received 15 mg/kg bodyweight DMBA and were treated with 15 and 30 mg/kg bodyweight of MOF6, respectively. Group 5 received 15 mg/kg bodyweight DMBA and was treated with 10 mg/kg bodyweight of MSF1. Group 6 received 15 mg/kg bodyweight DMBA and was treated with 3.35 mg/kg bodyweight of doxorubicin and intravenous injection of 0.5 ml/200 g of cisplatin. Groups 7–9 received only 15 and 30 mg/kg bodyweight of MOF6 and 10 mg/kg bodyweight of MSF1, respectively. DMBA, doxorubicin, and extracts doses were administered orally. The duration of our experimental procedure was 8 weeks. Consequently, liver histopathology (hematoxylin and eosin technique) and enzyme-linked immunosorbent assay homogenates’ concentrations of Ki67 and MDR1 were evaluated. Computed data were statistically analyzed (P ≤ 0.05). Results: Results showed normal histoarchitectures of the liver in all groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased concentrations (P ≥ 0.05) of Ki67 and MDR1 in Groups 3–9 compared with Group 2. Therefore, MOF6 and MSF1 ameliorated DMBA-induced hepatotoxicity, abnormal proliferation, and drug resistance. Conclusion: MOF6 and MSF1 possess antiproliferation, anti-drug resistance, and anticancer potentials.Item Moringa oleifera IS PROTECTIVE AGAINST MICROARCHITECTURAL AND NEUROCHEMICAL CHANGES ASSOCIATED WITH CUPRIZONEINDUCED PREFRONTAL CORTEX NEUROTOXICITY IN FEMALE WISTAR RATS(Neuroscience Society of Nigeria (NSN), 2018-05-20) Omotoso, G. O.; Gbadamosi, I. T.; Akinlolu, A, A.; Ameen, Mubarak; Kadir, R. E.; Jaji-Sulaimon, R.; Abdulwahab, A. B.; Kolo, R. M.Cuprizone administration causes selective damage to axonal myelin sheath and has been used to model demyelinating diseases in neuroscience research. This study aimed at determining the protective effects of Moringa oleifera on cuprizone-induced neurotoxicity in the prefrontal cortex (PFC). Sixteen adult female Wistar rats were procured and grouped into 4: Group A was given normal saline, Group B received 0.4% cuprizone diet, Group C was administered with 1.875 mg/ml of Moringa oleifera and Group D received a combination of 0.4% cuprizone diet and 1.875 mg/ml of Moringa oleifera. All the groups were treated orally for 35 consecutive days after which they were sacrificed. Thereafter the PFC was processed for histological demonstration, while tissue homogenate was used to assay the activity of superoxide dismutase (SOD). Cuprizone administration caused significant reduction in body weight and SOD activities. It also caused an alteration in the microarchitecture and Nissl profile of the PFC. Moringa oleifera intervention led to restoration of body weight, SOD levels, Nissl profile and the histology of the PFC. The use of preparations of Moringa oleifera, especially the leaf-component, could offer some protective measures to individuals suffering from demyelinating conditions, especially in addressing the associated weight changes and frontocortical dysfunction.Item Research Journal of Health Sciences(2018-02-01) Omotoso, G. O.; Kadir, R. E.; Gbadamosi, I. T.; Akinlolu, A. A.; Adunmo, G. O.; Kolo, R. M.; Lawal, M. O.; Ameen, M. O.Objectives: Cuprizone is a neurotoxicant used in modeling demyelinating disorders. This study explored the effects of Moringa oleifera (MO) on oxidative, histomorphological and behavioural changes in cuprizone-damaged cerebellum. Methods: Twenty adult female Wistar rats were grouped into 4, each group having five animals. Group A received 1 ml of normal saline (Control); group B received 0.4% cuprizone; group C received 15.6 mg/kgBW Moringa oleifera leaf extract; group D received 0.4% cuprizone and 15.6 mg/kgBW Moringa oleifera, orally for 5 weeks. The animals were assessed for exploratory and locomotor activities, while the cerebellum was processed for histology and assayed for nitric oxide (NO), catalase (CAT) and superoxide dismutase (SOD) activities. Results: Cuprizone treatment caused weight reduction, disruption of Purkinje cell layer, cellular degeneration, reduction in NO, CAT and SOD activities. However, these changes were ameliorated when co-administered with MO. Conclusion: The anti-oxidative property of Moringa oleifera is responsible for its ameliorative effect in cuprizone neurotoxicity.