Browsing by Author "KOLAWOLE, OLATUNJI"

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    Antimicrobial Activities of Vernonia amygdalina Against Oral Microbes
    (IDOSI Publications, 2012) ANIBIJUWON, IBIKUNLE; OLADEJO, BABAYEMI; ADETITUN, DAVID; KOLAWOLE, OLATUNJI
    Vernonia amygdalina was assessed for active principles. The results showed that the extract of the plant possessed the active principles e.g. terpenoids, tannins, alkaloids, saponins and glycosides. The antimicrobial activity of the extracts was assayed against Streptococcus mutans and Staphylococcus aureus, using the agar diffusion method. The extract from V. amygdalina show a very high antimicrobial activity because they somehow have a very low pH value. The ethanolic extract of V. amygdalina has the lowest minimum inhibitory concentration on Streptococcus mutans at 25mg/ml while aqueous extract of V. amygdalina had the highest value of MIC on Streptococcus mutans at 55mg/ml. The ethanolic and aqueous extract of V. amygdalina had their MIC at 60mg/ml on S. aureus which was the highest minimum inhibitory concentration on Staphylococcus aureus. Ethanolic extract of V. amygdalina had the lowest value on S. aureus at 40mg/ml. On the whole, most of the sensitive extract were only able to inhibit the growth of the organism which is known as bacteriostatic in action while some exert a killing effect on the test organism and suggests that the extracts from plants were bactericidal.
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    Bacteriological Analysis and Effect of Water Consumption on the Haematological Parameters in Rats
    (International Journal of Plant, Animal & Environmental Sciences., 2013) KOLAWOLE, OLATUNJI; ALAMU, FOLAKEMI; OLAYEMI, ALBERT; ADETITUN, DAVID
    Water samples were collected directly from Oyun river before entering the water treatment plant, chlorinated tank, storage tank, male hostel tap, and female hostel tap. Samples were analysed for their physicochemical characteristics, bacteriological load, as well as haematological studies on the rats fed with the water samples for thirty days. The pH of the sample ranged from 6.12 to 6.84, water temperature was between 27.80 C and 26.40 C inclusive, turbidity also ranged from 4.7(NTU) to 8.2(NTU), total dissolve solid ranged from 8.00mg/l to 85.20mg/l, BOD value for the untreated water was 4.150 and conductance ranged from 82.02µS/cm to 89.06µS/cm. Total heterotrophic plate count ranged from 1.33 to 34.00 cfu/ml, total coliform count ranged from 4 cfu/ 100ml to 192cfu/100ml and total thermotolerant coliform count ranged from 1.50 to 124.67 cfu/100ml. The organisms isolated are Escherichia coli, Klebsiella pneumoniae, Salmonella sp., Shigella sp., Yersinia enterocolitica, Enterobacter aerogenes, Serratia marcescens, Micrococcus varians, Proteus vulgaris, Streptococcus sp., Staphylococcus aureus, Pseudomonas aeruginosa, Lactobacillus sp. and Bacillus sp. Hematological investigations revealed the PCV value to ranged from 24.00 to 28.80, Hb (5.60 to 9.04), RBC (3.22 to 5.44), WBC (5.46 to 7.12), Lymphocyte count (55.40 to 61.00) and Neutrophil value was between 38.60 and 43.40 inclusive. We therefore advocate proper treatment of water before distribution to the consumers.
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    Bacteriological analysis of spent engine oil contaminated soil planted with cowpea (Vigna unguiculata)
    (Department of Geography and Environmental Studies, Bahir Dar University, Ethiopia, 2016) ADETITUN, DAVID; ADEBISI, OLUSOJI; ANIBIJUWON, IBIKUNLE; KOLAWOLE, OLATUNJI; ADEOSUN, T.B.; OLAYEMI, ALBERT
    The bacteriological analysis of soil contaminated with spent engine oil (SEO) planted with cowpea was investigated. The aim of this study was to detect the microbial degradation of SEO in soil and how it affects the microbial activity and the effects of SEO on the growth of cowpea. SEO collected from a mechanic workshop in Ilorin was introduced into soil in varying concentrations. The experimental set up was in triplicates with six treatments of SEO. Soil samples were taken every week for the duration of six weeks for laboratory analysis. Plant growth parameters were measured every week after planting. The pH of the soil and the bacterial population of the soil were also observed. Findings revealed that the plant growth parameters were significantly reduced as the concentration of SEO increased in the soil. Bacterial counts were also determined and a total of six bacterial species were isolated from the soil samples. Bacillus subtilis, Micrococcus luteus, Pseudomonas aeruginosa and Pseudomonas putida occurred in the control and contaminated soil samples. Bacterial counts ranged from 1.89×105 to 4.25×105 cfu/ml in the soil samples with the highest occurring in the control and the least occurring in 224ml of SEO. SEO contaminated soil has adverse effect on cowpea and on bacterial flora of the soil. The results of this study revealed that Pseudomonas aeruginosa, Micrococcus luteus and Bacillus subtilis can utilize SEO in the soil. They could be harnessed for use in bioremediation of soil polluted with petroleum and petroleum products.
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    Biodegradative activities of some gram- negative bacilli isolated from kerosene treated soil grown with cowpea (Vigna unguiculata)
    (Agrosearch, 2016) ADETITUN, DAVID; AWOYEMI, OLUSHOLA; ADEBISI, OLUSOJI; KOLAWOLE, OLATUNJI; OLAYEMI, ALBERT
    The biodegradative activities of three Gram negative bacilli (Aeromonas hydrophila, Vibro parahaemolyticus and Actinobacillus sp) isolated from soil contaminated with kerosene and planted with cowpea was investigated. The isolates were identified using MicrobactTM ID 24E system for the identification of Enterobacteriaceae and common miscellaneous Gram negative bacilli (MGNB). 2kg of soil collected from University of Ilorin was placed inside transparent, drilled buckets. Physicochemical properties of the soil were recorded. The soil was contaminated with kerosene at different concentrations of 0ml, 7ml, 14ml, 21ml, 56ml, 112ml, 168ml and 224ml. The setup was laid out in a randomized complete block design with three replicates. Cowpea seeds of the variety Samaru-40 were cultivated and observed for eight weeks. The results indicated that kerosene contamination of soils significantly affected the growth parameters: germination percentage, time of germination, rate of germination, plant height, number of leaves, leaf area and root nodules. A negative interaction existed between the level of the contaminant and the growth characteristics measured. Their biodegradative activities were studied and confirmed by the change in the Total Petroleum Hydrocarbon (TPH) using gravimetric method. The biodegradative abilities of the isolates were compared by measuring the optical densities, total viable count, pH and emulsification activity. The results showed that the organisms did better as a consortium rather than singly. Aeromonas hydrophila had the highest biodegradative activity followed by Vibro parahaemolyticus and then Actinobacillus sp. The study recommends the use of Aeromonas hydrophila and consortium for more effective biodegradation.
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    Hydrocarbon-degrading Capability of Bacteria isolated from a Maize-Planted, Kerosene-contaminated Ilorin Alfisol
    (Nigerian Society for Experimental Biology (NISEB)., 2014-03-31) ADETITUN, DAVID; OLAYEMI, ALBERT; KOLAWOLE, OLATUNJI
    In an effort at discovering autochthonous and active bacterial strains that could be of relevance in biodegradation and/or bioremediation of petroleum contaminated systems in Nigeria, twenty four bacterial species were isolated from kerosene treated Ilorin alfisol. The traditional method of identifying bacteria was complemented by using MicrobactTM ID 24E system for the identification of Enterobacteriaceae and common miscellaneous Gramnegative bacilli (MGNB). The results show appreciable increase in optical densities and total viable counts contemporaneous with decrease in pH of the culture media. The most promising organisms in this study are Leclercia adecarboxylata, Pseudomonas aeruginosa, Proteus mirabilis, Micrococcus luteus, Arthrobacter sp. and Streptococcus sp. The results obtained in this study showed that kerosene spillage posses a great threat to the survival and development of Zea mays. It also revealed that some bacteria survive and even thrive in kerosene contaminated soil and hence have the potential to be used in biodegradation and/or bioremediation of oil contaminated soils and water.
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    Molecular identification of hydrocarbon-degrading bacteria isolated from alfisol-loam experimentally-contaminated with gasoline
    (Nigerian Society for Experimental Biology (NISEB)., 2016-09) ADETITUN, DAVID; OLAYEMI, ALBERT; KOLAWOLE, OLATUNJI; FATHEPURE, BABU
    Impacts of gasoline pollution on the soil environment, bacterial and weed population dynamics were investigated for several months. Known amounts of gasoline were added to 2kg of soil. In the first experiment the soil was immediately sampled for microbial load and presence of weeds. In the second experiment the soil was sampled after three months for microbial load and presence of weeds. In the third experiment the soil was sampled after 6 months for microbial load and presence of weeds. The soil was sampled weekly for twelve weeks in each of the three experiments. Weeds that grew on the soil were noted and identified. Bacteria isolated were identified using 16S rRNA sequencing. The identified bacteria include Bacillus spp., Pseudomonas sp, and Ochrobactrum sp. The bacteria were further tested for their ability to grow on gasoline as the only source of carbon and their growth measured by optical density, change in pH and total viable counts. Results showed ability to utilize gasoline as the only source of carbon and energy. The emulsification activities of the isolates were greater than 50% indicating higher potential to biodegrade gasoline. It is concluded that it is possible to isolate oil degrading bacteria capable of in situ biodegradation from the Niger Delta region of Nigeria. It is suggested that the identified weeds have some roles to play in the biodegradation of gasoline by bacteria
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    Synthesis, thermal properties, and biological study of metal(II) nicotinamide complexes containing fumarate dianion and fumaric acid: Crystal structure of [Ni(H2O)4(nia)2](fum)·(H2fum)
    (Taylor and Francis, 2017) ADEDIBU, TELLA; OWALUDE, SAMSON; MEHLANA, GIFT; OLATUNJI, SUNDAY; ADETITUN, DAVID; KOLAWOLE, OLATUNJI; NZIKAHYEL, SIMON; ALIMI, LUKMAN
    New divalent transition metal nicotinamide (nia) complexes containing fumarate (fum) dianion and fumaric acid (H2fum), [M(H2O)4(nia)2](fum)·(H2fum) [M = Co (1), Cu (2), and Ni (3)] have been synthesized.The compounds were characterized by elemental analyses, infrared, UV-vis, XRPD, and TGA. Structural analysis of 3 using single-crystal X-ray diffraction technique revealed that the Ni(II) ion is coordinated by four aqua and two nia ligands in an octahedral geometry. The structure of 3 is completed with fumarate (fum2−) dianion acting as a counterion while fumaric acid (H2fum) is present as a molecule of solvation. The three complexes were investigated for biological activities.