Browsing by Author "Jimoh, Fausat Abimbola"

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    Extraction and Antibacterial Activity of Essential Oils from Eucalyptus globulus
    (Ilorin Journal of Science, 2016) Ahmed, Risikat Nike; Sule, Ismaila Olawale; Zakariyah, Rahmat Folashade; Gambari-Ambali, Rabiat O; Jimoh, Fausat Abimbola
    The essential oil of Eucalyptus globulus obtained by hydrodistillation (Fever tree) was investigated for activity against two bacteria (Staphylococcus aureus and Klebsiella pneumoniae). The oil was extracted using a Clevenger-type apparatus. Separation, identification and determination of percentage composition of compounds present in the oil were carried out by Gas Chromatography Mass Spectrometry. The antibacterial activity was investigated by agar diffusion technique using various concentrations of the essential oil: 100% v/v, 75% v/v, 50% v/v, 25% v/v, 15% v/v. Minimum inhibitory concentration was determined using turbidimetric assay by taking the absorbance with the aid of a spectrophotometer. Minimum bactericidal concentration was by plate assay. Gas Chromatography Mass Spectrometry revealed the presence of seven compounds, all amounting to 90.4% with globulol having the highest percentage composition of 30.1%. The evaluation of sensitivity of the oil revealed the highest activity at 100% v/v against both isolates. Minimum inhibitory concentration was obtained at 25% v/v when assayed against K. pneumoniae with no activity at all against S. aureus at the same concentration. Oil showed no bactericidal activity at all concentrations, suggesting only bacteristatic activity of the oil. Investigation from this work revealed that the essential oil of E. globulus exhibit activity against the tested isolates and can thus be employed in the treatment of infections caused by them.
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    MICROBIOLOGICAL AND PHYSIOCHEMICAL ASSESSMENT OF STREET VENDED FRIED SOYABEAN CHEESE SOLD WITHIN ILORIN METROPOLIS, NIGERIA.
    (Journal of Science, Technology, Mathematics and Education (JOSTMED), 2016-03) Odebisi-Omokanye, Mutiat Busayo; Zakariyah, Rahmat Folashade; Jimoh, Fausat Abimbola; Olugbade, O.F
    The microbiological and physicochemical quality of street vended fried soyabean cheese products from five different locations was assessed. Standard pour plate technique was used to examine the microbial load of the soyabean cheese. The pH ranged from 6.48 + 0.241 – 6.58 + 0.192, Moisture content ranged from 13.94% + 0.152 – 14.22% + 0.179. The viable bacterial counts ranged from 7.0 to 61 x 105 cfu/g, while the fungal load ranged from 1 to 40 x 103 cfu/g. Eight bacteria species were isolated and identified as Bacillus subtilis, Escherichia coli, Micrococcus sp., Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus, Enterobacter aerogenes and Klebsiella pneumoniae. Similarly, four fungal species were isolated and identified as Mucor racemosus, Aspergillus fumigatus, Saccharomyces cerevisiae, and Rhizopus stolonifer. The results suggest that the soyabean cheese samples were contaminated with pathogenic microorganisms and could cause health hazard to the consumers. There is the need to improve personal hygiene and environmental sanitation during and after production of the soyabean cheese.
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    SCREENING OF Prosopis africana FOR ANTIMICROBIAL ACTIVITY AGAINST SELECTED ORAL PATHOGENS
    (Nigeria Journal of Pure and Applied Sciences, 2017) Zakariyah, Rahmat Folashade; Sani, Al-hassan; Odebisi-Omokanye, Mutiat Busayo; Ahmed, Risikat Nike; Jimoh, Fausat Abimbola; Badmus, Simbiat Atinuke
    Prosopis africana, a chewing stick popularly used in the South Western part of Nigeria was examined for antimicrobial activity against Lactobacillus casei ATCC 334, Staphylococcus aureus ATCC 35218, Candida albicans ATCC 10231, Escherichia coli ST2747 and Streptococcus mutans using agar well diffusion method. Extraction was done using ethanol and distilled water. The plant was screened for its phytochemical components. Gas chromatography mass spectrometry analysis was done to detect the secondary metabolites. All the extracts showed antimicrobial activity against the organisms with zones of inhibition ranging from 3.33±0.29 - 13.83±1.61mm except Staphylococcus aureus ATCC 35218 which was resistant to aqueous extract of the dried sample. Statistical analysis of data showed that there was no significant difference in the antimicrobial effects of ethanolic and aqueous extracts of the plant samples at 95% confidence intervals. The phytochemical screening result showed that phenols, alkaloids, sterols, triterpenes and saponins are components of the chewing stick. Also, fatty acids such oleic, palmitic and stearic acids were chemical constituents detected in the plant extracts by GC-MS. The results obtained in this study indicate that P. africana will be useful in the treatment of oral infections.