Browsing by Author "Hamid, A. A."

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    A study on endophytic fungi, proximate and chemical compositions of local variety of mango fruit (Mangifera indica L.)
    (Journal of the University of Ruhuna, Sri Lanka, 2018) Garuba, T.; Olayinka, B. U.; Abdulkareem, K. A.; Bello, M. O.; Hamid, A. A.; Olaleye, F. Y.
    Mango (Mangifera indica L.) is an important tree which produce edible fruits. Different varieties of mango are available and well-studied. However, little attention has been shown to popular Ogbomoso variety especially for its endophytes and chemical profile. This work aimed at identifying endophytic fungi and carrying out proximate analysis as well as phytochemical analysis of the fruit. Potato Dextrose Agar was used as a medium for the isolation of fungi from the homogenized pulp. Proximate analysis was carried out using standard method of Association of Analytical Chemistry (AOAC). Fourier Transform Infrared Spectroscopy (FTIR) was done to determine characteristic peaks and functional groups of compounds using Thermo Scientific Nicolet iS5 with iD1 transmission. Gas Chromatography- Mass Spectrometry (GC-MS) was used to study the chemical profile of the sample. Aspergillus niger. A. flavus and Rhizopus stolonifer were the identified endophytes. Moisture and lipid were observed to be highest (76.87%) and lowest (0.38%) respectively in the sample. The amount of carbohydrate was 18.93%. The FTIR result revealed the presence of NH2, OH, CH2, C=O (carboxylic) and N-H 1o and 2o amine as important functional groups. A total of 23 compounds were found in the methanolic extract of the sample where Imidazolidin-2-one (18.43%) was the principal compound. N, N-dibenzylhydroxylamine (15.36%), Methyl hexadecanoate (7.33%), Hexadecamethylcyclooctasiloxane (4.51%) were also present in considerable quantities. It is confirmed that the homogenized pulp of Ogbomoso variety of mango fruit is rich in phytochemicals that are useful especially in pharmaceutical industries.
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    Chemical constituents and antiproliferative properties of Turraea vogelli Hook. f. ex. Benth leaves.
    (University of Ilorin, 2015) Hamid, A. A.; Negi, A. S.; Zubair, M. F.; Oguntoye, S. O.; Aiyelaagbe, O. O
    Ethyl acetate and methanol extracts of Turraea vogelli leaves exhibited cytotoxic activity on leukaemia carcinoma K562 with IC50 values 85.00 and 85.22 μg/mL respectively. Isolation of the extracts afforded Tetradec-7-enoic acid (1), pentadec-1-ene (2), ethyl tridec-7-enoate (3), β-sitosterol (4) and stigmasterol (5). The structures of these compounds were characterised by IR, ID and 2D NMR and Mass spectroscopy, and supported with literature data. Compounds 1 and 4 exhibited antiproliferative activity against K562 tumor cell lines with IC50 of 57.27 and 27.56 μg/mL respectively. All isolated compounds exhibited low cytotoxicity against WRL and MCF-7 tumor cell lines.
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    Chemical Constituentss of Leaf Essential Oil of North-central Nigerian Grown Vitex Agnus-castus I
    (American-Eurasian Network for Scientific Information, 2010) Hamid, A. A.; Usman, L.A; Adebayo, S.A.; Zubair, M.F; Elaigwu, S.E
    Pulverized leaf of vitex agnus-castus on hydrodistillation, afforded oil in the yield of 0.8%v/w. GC, GC/MS analyses of the oil revealed the abundance of hydrocarbon and oxygenated monoterpenes (53.2 and 24.5% respectively). The major constituents of the oil were; β-pinene (20.0%), viridiflorol (9.8%), α-pinene (9.1%), cis-ocimene (8.4%), 1,8-cineole (6.7%), β-farnesene (5.4%), terpinen-4-ol (4.2%), α-terpineol (4.1%) and β-phellandrene (4.1%).
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    Preliminary Phytochemistry, Antibacterial and Antifungal Properties of extract of Asystasia gangetic Linn T. Anderson grown in Nigeria
    (Pelagia, 2011) Hamid, A. A.; Aiyelaagbe, O. O.; Ahmed, R. N.; Usman, L. A.; Adebayo, S. A
    The hexane, ethylacetate and methanol extracts obtained from the whole plant of Asystasia gangetica were evaluated invitro to determine inhibition of human pathogenic microorganisms made up of six bacteria and six fungi. The crude extracts inhibited the growth of twelve test organisms to different degrees. All the bacteria strains were sensitive to all the extracts at concentration ranging from 50 to 200mg/mL using the agar diffusion pour plate method. The inhibition of these test organisms were concentration dependent, activity being higher at higher concentration of all the three extract. The extracts showed higher antifungal properties on Candida albicans, Penicillum notatum, Tricophyton rubrum and Epidermophyton floccosum with activity comparable to that of the reference drug. Tioconazole, Preliminary phytochemical investigation of the extracts revealed the presence of saponins, reducing sugar, steroids, glycosides, flavonoids and anthraquinones.