Browsing by Author "Garuba, T"

Now showing 1 - 3 of 3
  • Item
    Microorganisms associated with some selected leafy vegetables sold in some markets in Ilorin, Kwara State
    (Department of Biological Sciences, Faculty of Science, Bayero University, Kano, Nigeria, 2014-06-06) Olahan, G.S; Garuba, T; Adeyemi, S.B.; Lateef, A.A.; Olahan, S.A.
    Microorganisms associated with some commonly consumed leafy vegetables in popular markets of Ilorin, kwara State were investigated. The leafy vegetables were Amaranthus hybridus, Brassica oleraceae and Telfairia occidentalis. Seven species of bacteria were isolated namely Escherichia Coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Enterobacter aerogenes, streptococcus faecalis and Micrococcus luteus. Six species of fungi were equally isolated from some of the same leafy vegetables namely Aspergillus niger, A. Flavus, Rhizopus stolonifer, Neurospora sitophila, penicillium chrysogenum and Mucor racemosus. The isolates which are pathogenic might have arisen from conditions in the field and gardens where the leafy vegetables were grown, during transportation of the leafy vegetables to the market or as a result of the filthy environment in the markets where the leafy vegetables were obtained for this study. Therefore there is a great need for increased awareness on environmental hygiene in those fields and gardens and the market as well as sensitization of the farmers and traders on the need to adopt the modern technologies of transporting, storing and handling leafy vegetables.
  • Item
    Molecular Characterization of Potential Crop Pathogens Associated with Weeds as Endophytes in Uniilorin Plantations, Nigeria
    (College of Science for Women/ University of Baghdad, 2022-05-20) Lateef, A.A; Garuba, T; Abdulkareem, K.A; Olayinka, B.U; Olahan, G.S; Adeyemi, S.B; Abdulrahaman, A.A
    Crop diseases are usually caused by inoculum of pathogens which might exist on alternate hosts or weeds as endophytes. These endophytes, cum pathogens, usually confer some beneficial attributes to these weeds or alternate hosts from protection against herbivores, disease resistance, stress tolerance to secondary metabolites production. This study was therefore carried out to isolate potential crop pathogens which exist as endophytes on weed species in the University of Ilorin plantations. Green asymptomatic leaves were collected from 10 weed species across the plantations, and processed for their endophytic fungi isolation. Isolates were purified into pure cultures and used for molecular identification using the internal transcribed spacer (ITS) region of the ribosomal DNA. Phylogenetic analysis of the fungal sequences using MEGA software revealed 9 fungal genera belonging to 13 species, with species in the genera Curvularia, Epicoccumand Daldinia occurring in more than one weed species, while other genera such as Alternaria, Fusarium, Chaetomium, Macrophomina, Arthrinium and Phomopsis occurred in just one weed species each. Daldinia eschscholtzii was isolated in this study as an endophyte from Loudetia arundinacea for the first time. This plant is very abundant in Nigeria and Africa where it is used majorly for thatching and feeding livestocks. This also represents the first endophytic fungi from the genus Loudetia. Potential relationship between the occurrences of these fungi as endophytes and as pathogens are discussed. These discoveries represent the first large-scale molecular identification and several first reports of endophytes from these weed species. These results also represent the first records of some of these fungi in Nigeria.
  • Item
    Mutagenic Role of Artificial Ultraviolet (UV-C) Irradiation on the Growth and Yield of Tomato (Solanum Lycopersicon L.)
    (Uka Tarsadia University, Bardoli, Gujarat, India, 2015-07-31) Abdulkareem, K.A; Mustapha, O.T; Garuba, T; Kasture, A; Oyeyiola, O.B
    Effects of artificial ultraviolet (UV-C) radiation (254nm) on the growth and yield of Solanum lycopersicon (tomato) accession NGB 01301 were studied. Viable seeds were irradiated with handheld UV lamp (model UVGL 55; Science company, USA) for 0, 10, 20, 30, 40, 50 and 60 min. Treated seeds were sown in plastic buckets at the screened house of the Botanical garden of the University of Ilorin, Nigeria in five replicates. Quantitative data were obtained at maturity, 20 WAS (Weeks After Sowing) and subjected to Analysis of variance using SPSS 16.0 for Windows. Highest germination (93.33%) was obtained in 30 min treatment and the lowest (63.33%) in 10 min exposure. Maximum plant height (53.37 cm) was recorded in 10 min while the least (47.60cm) was in 60 min treatment. Petiole length was highest (2.40 cm) in treatment for 10min, treatment with 50 min produced least petiole length (1.37±0.26 cm). With respect to leaf parameters, optimum performance was recorded in 30 min treatment. However, highest performance in response to UV irradiation with respect to petiole length, number of branches and leaf number occurred in 10 min treatment. Among the exposure time evaluated, 30 min produced early maturing plants with highest fruit yield.