Browsing by Author "Enaibe, B. U."

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    Kolaviron ameliorates toxic effects of aluminum chloride on the hippocampus of fetal Wistar rats in utero: Biochemical and ultrastructural observations.
    (Jagadguru Sri Shivarathreeshwara University, Mysore., 2021-12-17) Lewu, F. S.; Enaibe, B. U.
    BACKGROUND: This study was designed to investigate the biochemical and ultrastructural effects of kolaviron (Kv) on the hippocampus of fetal Wistar rats exposed to aluminum chloride (AlCl3) toxicity in utero. MATERIALS AND METHODS: Fifty female Wistar rats were selected at random and mated. Following confirmation of mating, pregnant rats were assigned into five groups (n = 5). Controls: Group A received distilled water; Group B: 0.6 ml of corn oil; Group C: 200 mg/kg of Kv; Group D: 100 mg/ kg of AlCl3 and Group E 100 mg/kg of AlCl3 and 200 mg/kg of Kv. Administration was done from days 8-10 and 15-17 of gestation during the 2nd and 3rd weeks respectively. Biochemical analyses were investigated to assess oxidative stress levels, while transmission electron microscopy (TEM) examined ultrastructural changes. Pregnant animals were sacrificed on day 20 of gestation; fetuses, their brains, and hippocampi were excised, respectively. Hippocampal tissues of fetuses were homogenized in 0.25 M of sucrose solution for biochemical assay while some were fixed in 2.5% phosphate‑buffered saline‑based glutaraldehyde for TEM. RESULTS: Elevated levels of Al, malondialdehyde, nitric oxide, and interleukin 6 were observed in the hippocampi of fetuses whose mothers received AlCl3. TEM revealed loss of nuclear membrane and increased condensation of chromatin materials in the same group. However, significant reduction of these enzymes including improved ultrastructural alterations were observed in the fetal hippocampus of the AlCl3 + Kv‑treated group. CONCLUSION: This study showed that Kv significantly reduced neurodegenerative effects induced by AlCl3 in the hippocampii of fetal Wistar rats in utero probably owing to its antioxidant and anti‑inflammatory properties.
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    Moringa oleifera Impairs the Morphology and Functions of the Kidney in Adult Wistar Rats
    (Anatomical Society of Chile, 2014-09-10) Akinlolu, A. A.; Ghazali, O. K.; Ameen, Mubarak; Oyebanji, S. C.; Omotoso, G. O.; Enaibe, B. U.
    We tested the hypothesis that Moringa oleifera impairs the morphology and functions of the kidney in rats. Twenty four adult male Wistar rats were employed in the study. Rats of Control Group I received physiological saline while rats of Groups II – IV received 250, 500 and 750 mg/kg body weight of methanolic extract of Moringa oleifera respectively for twenty one days. No behavioral anomalies were observed in rats of Groups I – IV. Rats of Control Group I gained statistically significant increased body weight while rats of Groups II – IV experienced non-significant decreased body weight during experimental procedure. (P£0.05). No statistical significant differences (P£0.05) were observed in the analyses of the relative weights of kidneys of rats of Groups I – IV. Histological examinations showed normal cyto-architecture of the kidneys of rats of Group I while the Capsular spaces of the kidneys of rats of Groups II – IV appeared wider than those of Group I. Statistical analyses showed significant higher levels (P£0.05) of Alanine and Aspartate Transaminases, and serum urea in rats of Groups II – IV in a non- dose-dependent manner when compared to rats of Group I. Our findings are consistent with the stated hypothesis.