Browsing by Author "Ebito, G. E."
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Item Moringa oleifera and Musa sapientum ameliorated 7,12-Dimethylbenz[a]anthracene-induced upregulations of Ki67 and multidrug resistance 1 genes in rats(Qassim University, 2021-05) Akinlolu, A. A.; Oyewopo, A. O.; Kadir, R. E.; Lawal, A.; Ademiloye, J.; Jubril, A.; Ameen, M. O.; Ebito, G. E.Objectives: Moringa oleifera (MO) and Musa sapientum (MS) are plants of ethnomedicinal importance. We evaluated the effects of MOF6 (extracted from MO leaves) and MSF1 (extracted from MS suckers) on immunomodulations of Ki67 (proliferation biomarker) and multidrug resistance 1 (MDR1) genes in the liver of rats in 7,12-Dimethylbenz[a]anthracene (DMBA)-induced hepatotoxicity and mutagenesis to determine their antiproliferation, anti-drug resistance, and anticancer potentials. Methods: Forty-five adult male rats were randomly divided into nine groups (n = 5). Groups 1 and 2 received physiological saline and 15 mg/kg bodyweight of DMBA, respectively. Groups 3 and 4 received 15 mg/kg bodyweight DMBA and were treated with 15 and 30 mg/kg bodyweight of MOF6, respectively. Group 5 received 15 mg/kg bodyweight DMBA and was treated with 10 mg/kg bodyweight of MSF1. Group 6 received 15 mg/kg bodyweight DMBA and was treated with 3.35 mg/kg bodyweight of doxorubicin and intravenous injection of 0.5 ml/200 g of cisplatin. Groups 7–9 received only 15 and 30 mg/kg bodyweight of MOF6 and 10 mg/kg bodyweight of MSF1, respectively. DMBA, doxorubicin, and extracts doses were administered orally. The duration of our experimental procedure was 8 weeks. Consequently, liver histopathology (hematoxylin and eosin technique) and enzyme-linked immunosorbent assay homogenates’ concentrations of Ki67 and MDR1 were evaluated. Computed data were statistically analyzed (P ≤ 0.05). Results: Results showed normal histoarchitectures of the liver in all groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased concentrations (P ≥ 0.05) of Ki67 and MDR1 in Groups 3–9 compared with Group 2. Therefore, MOF6 and MSF1 ameliorated DMBA-induced hepatotoxicity, abnormal proliferation, and drug resistance. Conclusion: MOF6 and MSF1 possess antiproliferation, anti-drug resistance, and anticancer potentials.Item PHYTOCHEMICAL SCREENING, ANTI-OXIDANT AND ANTI-MICROBIAL ACTIVITIES OF FRACTIONATED ETHANOLIC EXTRACT OF Moringa Oleifera LEAVE(Chemical Society of Nigeria, 2021) Ameen, O. M.; Akinolu, A. A.; Garuba, T.; Ebito, G. E.; Arowolo, B. Z.; Oyebanji, E. O.Moringa oleifera leave has been reputed for the treatment of different ailments, this work thus aims at isolating the active principle(s) that are responsible for these activities. M. oleifera leave was extracted with distilled ethanol, concentrated to afford the crude extract (210.2 g). The crude extract was partitioned using n – hexane (NH), dichloromethane (DCM), ethyl acetate (EA) and methanol (MeOH) in an increasing order of polarity to afford 12 fractions. The fractions were subjected to biological testing and MOF8 and MOF11 were selected for further purification based on their antioxidant and antimicrobial activities. The two fractions (MOF8 and MOF11) were purified on a silica gel open column, using NH, DCM, EA and MeOH in an increasing order of polarity to afford ten and eight fractions respectively. Further biological test on these fractions indicates superior activities of MOF118 which was further purified as described earlier to afford four fractions. Further biological testing on these fractions indicates that and MOF118.3 and MO118.4 are more active in the antioxidant and antimicrobial analysis. Phytochemical screening of MOF11 and MOF11 indicates the presence of flavonoids, saponin, tannins, alkaloids, glycosides and steroids.Item PHYTOCHEMICAL SCREENING, ANTI-OXIDANT AND ANTI-MICROBIAL ACTIVITIES OF FRACTIONATED ETHANOLIC EXTRACT OF Moringa Oleifera LEAVE(Chemical Society of Nigeria, 2021) Ameen, Mubarak O.; Akinolu, A. A.; Garuba, Taofeeq; Ebito, G. E.; Arowolo, Zainab B; Oyebanji, E. O.Moringa oleifera leave has been reputed for the treatment of different ailments, this work thus aims at isolating the active principle(s) that are responsible for these activities. M. oleifera leave was extracted with distilled ethanol, concentrated to afford the crude extract (210.2 g). The crude extract was partitioned using n – hexane (NH), dichloromethane (DCM), ethyl acetate (EA) and methanol (MeOH) in an increasing order of polarity to afford 12 fractions. The fractions were subjected to biological testing and MOF8 and MOF11 were selected for further purification based on their antioxidant and antimicrobial activities. The two fractions (MOF8 and MOF11) were purified on a silica gel open column, using NH, DCM, EA and MeOH in an increasing order of polarity to afford ten and eight fractions respectively. Further biological test on these fractions indicates superior activities of MOF118 which was further purified as described earlier to afford four fractions. Further biological testing on these fractions indicates that and MOF118.3 and MO118.4 are more active in the antioxidant and antimicrobial analysis. Phytochemical screening of MOF11 and MOF11 indicates the presence of flavonoids, saponin, tannins, alkaloids, glycosides and steroids.