Browsing by Author "David, Susan M"
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Item Bacteriological Quality of Locally Fermented Milk (Nono) and Commercial Yoghurts Marketted in Ilorin(Journal of Pharmaceutical Research, Development and Practice, 2016) Shittu, Abiodun O; David, Susan M; Aliyu, Abdulmalik; Bello, Ronke H; Olajide, R.OEleven samples comprising of six branded yoghurts and five unbranded „nono‟ purchased from seven different locations randomly selected in Ilorin city were evaluated for their bacteriological quality. The physicochemical properties were determined using standard methods. Results for the total bacterial count for yoghurt ranged from 3.08 × 102 cfu/mL to 9.67×102cfu/mL while that of „nono‟ ranged from 1.056×103cfu/mL to 3.152×103cfu/mL. Eleven bacteria species were identified in this study. A total of forty-five isolates were obtained with Staphylococcus aureus (17.8%) and Pasturella multocida (17.8%) having the highest frequency. Others were Aeromonas hydrophilia (15.6%) Klebsiella oxytoca, Burkholderia pseudomallei, Actinobacillus spp (8.9%), Xanthomonas maltophilia (6.7%) Klebsiella pneumoniae, Escherichia coli and Salmonella (4.4%) and Pseudomonas aeruginosa (2.2%). Marketed yoghurts and locally produced „Nono‟ in Ilorin were found to be contaminated with mostly Gram negative bacteria belonging majorly to the Enterobacteriaceae family and a Gram positive organism (Staphylococcus aureus). The yoghurt and „nono‟ samples were found to be of unacceptable microbiological quality. The presence of these pathogenic organisms in these commonly consumed dairy products could constitute public health hazards and possibly food poisoining especially if they are stored under inappropriate conditions for longer periods of time.Item Biofilm Detection and Antibacterial Susceptibility Pattern of Isolates from In-Patients with Urinary Tract Infection in A Tertiary Hospital(Journal of Pharmaceutical Research, Development and Practice, 2018) Aliyu, Abdulmalik; Bello, Ronke H; David, Susan M; Olufadi-Ahmed, Haisat Y; Salami, Omoseye; Itodo, Ene PA survey for the detection of biofilms amongst uropathogens was conducted in a Medical ward of a Tertiary hospital in Ilorin, Kwara State. This was done to determine the prevalence of biofilm producing uropathogens among patients with CA-UTI (Catheter-Associated Urinary Tract Infection) using MTP (microtitre plate) method. Effect of media variations in the production of biofilms and the antibiogram pattern of the isolates were also determined. Out of the 50 urine samples collected and analyzed, 27 (54.0 %) yielded growth and 28 isolates were identified. Klebsiella pneumoniae was the most prevalent (42.9 %), followed by Staphylococcus aureus (35.7 %) and Pseudomonas aeruginosa was the least (21.4 %). A total of 59. 3 % of the isolates were resistant to various antibiotics used and 39.3 % of the isolates were found to be multiple antibiotic resistant. Of the 28 uropathogens isolated, 21 (75.0 %) were biofilm formers comprising of 17.9 % high biofilm formers and 57.1 % of moderate biofilm formers while 25.0 % did not form biofilm in BHIsuc. High biofilm formation was observed in Staphylococcus aureus. There was variation in biofilm formation with different media as more isolates (75. 0 %) produced biofilm in BHIsuc than in TSBglu (67.9 %). Clinical isolates of Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa have potential of biofilm production which could lead to relapse of disease condition and eventually treatment failure in CA-UTI.Item Plasmid Profile of Multidrug Resistant Isolates from Water and Fish(Journal of Pharmaceutical Research, Development and Practice, 2018) Aliyu, Abdulmalik; Ibrahim, Yakubu K.E; Oyi, Ruqqaya O; David, Susan M; Olufadi-Ahmed, Haisat Y; Bello, Ronke HPlasmids are extrachromosomal genetic material that can replicate independently and usually harbour antibiotic resistance genes. This study evaluated the presence and sizes of plasmid from multiple antibiotic resistant (MAR) isolates from water and fish using acridine orange as a plasmid curing agent. The minimum inhibitory concentration (MIC) of acridine orange to the 22 MAR ranged between 0.625 – 5.0 mg/mL. Majority (86.4 %) of the MAR isolates lost their resistant and became susceptible to more antibiotics indicating that their resistance was plasmid encoded. As much as 68.4 % of the isolates had one or more plasmid bands with sizes varying from 1.0 -3.0 kbp. The presence of plasmids - carrying MAR isolates in fish and river may heighten the dissemination of antibiotic resistance genes to the environment which will pose a serious threat to the human health.