Browsing by Author "Dada-Habeeb, SO"
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Item Moringa oleifera phytochemicals protect the brain against experimental nicotine-induced neurobehavioural disturbances and cerebellar degeneration.(Pathophysiology, 2018) Omotoso, Gabriel Olaiya; Gbadamosi, IT; Olajide, OJ; Dada-Habeeb, SO; Arogundade, TT; Yawson, EONicotine is a neuro-stimulant that has been implicated in the pathophysiology of many brain diseases.The need to prevent or alleviate the resulting dysfunction is therefore paramount, which has also given way to the use of medicinal plants in the management of brain conditions. This study was designed to determine the histomorphological and neurobehavioural changes in the cerebellum of Wistar rats fol-lowing nicotine insult and how such injuries respond to Moringa intervention. Twenty-four adult male Wistar rats were divided into 4 groups. Group A and B were orally treated with normal saline and Moringa oleifera respectively for twenty-eight days; Group C was treated with nicotine while group D was treated orally with Moringa oleifera and intraperitoneally with nicotine for twenty-eight days. Animals were subjected to the open field test on the last day of treatment. 24 h after last day treatment, the animals were anesthetized and perfusion fixation was carried out. The cerebellum was excised and post-fixed in 4%paraformaldehyde and thereafter put through routine histological procedures. Results revealed cytoarchitectural distortion and extreme chromatolysis in neuronal cells of the cerebellar cortical layers in the nicotine-treated group. The Purkinje cells of the cerebellum of animals in this group were degenerated.There were also reduced locomotor activities in the group. Moringa was able to prevent the chromatolysis, distortion of the cerebellar cortical cells and neurobehavioural deficit. Our result suggests that Moringa oleifera could prevent nicotine-induced cerebellar injury in Wistar rats,Item Moringa protects against nicotine-induced morphological and oxidative damage in the frontal cortex of Wistar rats(Anatomy, 2016) Gbadamosi, IT; Omotoso, Gabriel Olaiya; Olajide, OJ; Dada-Habeeb, SO; Arogundade, TTThe use of nicotine-containing substances has been implicated in oxidative-induced neuronal damage in several neurological dysfunctions. This study assessed the antioxidant potentials of Moringa tea on the frontal cortex of Wistar rats. Twenty female Wistar rats were divided into 4 groups of 5 animals each. Group A (control) received normal saline, Group B received 5.71 mg/kg of Moringa tea, Group C was treated with 13.76 mg/kg nicotine, while Group D received 5.71 mg/kg of Moringa tea and 13.76 mg/kg nicotine, for 21 days. Homogenate of excised frontal cortex of rats obtained on day 22 was used to assess the level of malondialdehyde, catalase, superoxide dismutase and glutathione peroxidase, while histological sections were stained with Heamatoxylin and Eosin. Results showed increased activities of malondialdehyde and catalase in group C and a slight increase in group D compared with the Control, while the activity of superoxide dismutase and glutathione peroxidase was reduced. The histological sections showed a normal architecture of the frontal cortex of rats treated with Moringa tea, but disrupted in the group treated with Moringa tea and nicotine and further distortion in those that received nicotine only, when compared with the control group. The results suggest that Moringa tea may reduce the oxidative stress associated with nicotine consumption and limit the extent of structural damage in the frontal cortex of Wistar rats.