Browsing by Author "Bakare-Odunola, M. T."

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    Phytochemical, Anti-oxidant and In-vitro Anti-diabetic Evaluations of Aqueous and Boiled Leaf Extracts of Thaumatococcus daniellii (Benth)
    (Ilorin Journal of Science, 2027) Njinga, N. S.; Ndifor, A. R.; Shittu, A. O.; Muhammadbashir, L.; Kayode, O.; Ohunene, S. R.; Mbakop, C.; Egharevba, G. O.; Bamidele, O. D.; Abdulrazaq, S.; Abdulazeez, I; Giwa, H. B.; Bakare-Odunola, M. T.; Kola-Mustapha A. T.
    Abstract Thaumatococcus daniellii (Benth), commonly referred to as the "miracle fruit plant," is traditionally used in cooking, where its leaves serve as natural food wrappers and flavour enhancers. This study investigate the scientific benefits of using T. daniellii in cooking by analyzing the phytochemistry, antioxidant, and in vitro anti diabetic properties of the fresh leaf boiled extract (FBE) and dried leaf aqueous extracts (DAE). Quantitative Phytochemical screening was done using spectrophotometric method. The antioxidant potential was evaluated through assays such as 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2'-azino-bis(3 ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, ferric-reducing antioxidant power (FRAP), peroxide-mediated antioxidant (PMA) activity, and metal chelating activity (MCA). The in vitro anti-diabetic potential was assessed using α-amylase inhibition assays to determine the potency of the extract in modulating glucose metabolism. Phytochemical analysis revealed distinct differences between the extracts. FBE had lower flavonoid (0.368 ± 0.023 mg QE/g) and alkaloid (642.44 ± 15.56 mg AE/g) content than DAE (0.472 ± 0.00523 mg QE/g and 665.78 ± 35.29 mg AE/g, respectively). Conversely, FBE showed higher tannin (278.13 ± 27.36 mg TAE/g) and phenol (0.048 ± 0.003 mg GAE/g) levels compared to DAE. Antioxidant assays highlighted varied results. FBE exhibited higher DPPH radical scavenging activity (36.72 ± 1.84%) and ferric-reducing antioxidant power (0.1895 ± 0.007 mg Fe²⁺/g) than DAE, while DAE demonstrated superior peroxide-mediated antioxidant activity (0.069 ± 0.0002 mg AAE/g). Metal chelating activity was similar for both extracts, and neither showed significant ABTS radical scavenging activity. The α-amylase inhibition assay revealed DAE's significant higher in vitro anti-diabetic potential (55.56 ± 2.26%) compared to FBE (12.82 ± 2.56%), suggesting that boiling diminishes this property.This study demonstrates that T. daniellii retains significant health-promoting bioactive properties, regardless of preparation method, making it a valuable addition to culinary and therapeutic applications. Keywords: Thaumatococcus daniellii, phytochemistry, Antioxidant activity, Anti-diabetic activity
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    Phytochemical, elemental, antioxidant, antimicrobial and hypoglycemic studies of a mixed herbal product used for the management of diabetics.
    (Journal of Pharmacy and Bioresources. Published by the Faculty of Pharmaceutical Sciences, University of Jos., 2018) Bakare-Odunola, M. T.; Njinga, N. S.; Ayaniyi, R. O.; Bello, M. K.; Abdullahi, S. T.; Eniayewu, O. I.; Abdulmajeed, F. F.; Bello, H. R.
    Medicinal plants are important sources of disease-preventing compounds, which are important for the treatment of various health challenges such as diabetes. On an aqueous extract of a herbal product (HP) used for the management of diabetes, total phenolic and flavonoid contents were determined by Folin -Ciocalteu reagent and AlCl3 method respectively. Microbiological evaluation was done by determining the total viable, yeast, mould and coliform bacteria count. The elemental analysis was carried out using atomic absorption spectrometer. The acute toxicity was done using Organization for Economic Cooperation and Development guideline while the hypoglycemic activity was evaluated using alloxan-induced diabetic rats. Flavonoids, saponins, alkaloid, cardiac glycoside, steroids and terpenoids were detected in the HP. Total flavonoid and phen olic contents obtained was 1.58±0.001mg/g quercetin equivalent and 10.84±0.003 mg/g gallic acid equivalent respectively. Heavy metals Fe and Zn were present while Cu, Cd, Cr and Pb were absent. Na and K were also present at concentrations of 3.90 and 2.20mg·kg−1 respectively. The total viable and coliform counts were found to be 1.34 x 105and 9.0 x 104 cfu/g respectively while there was absence of mould and yeast in the HP. The LD50 of the HP was found to be above 5000 mg/kg. At dose of 125 mg/kg, the HP significantly (P<005) reduced glucose level to 143 mg/dL after 4 hours and to 123 mg/dL after 8 hours. The phytochemicals present, safety and the anti-diabetic activity justify the use of this HP in the management of diabetes.
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    Proteinease Inhibitory, Membrane Stabilization, Phytochemical and Antioxidant Evaluations of Leaves, Seeds and Calyces of Four Selected Edible Medicinal Plants.
    (Cogent Chemistry (Taylor and Francis Group), 2017) Kambizi, L; Bakare-Odunola, M. T.; Oladiji, A. T.; Kola-Mustapha, A. T.; Amusa, T. O.; Atolani, O; Njinga, N. S.; Quadri, A. L.
    The aqueous extracts of the edible parts of four common medicinal plants (Hibiscus sabdariffa, Telfairia occidentalis, Moringa oleifera and Ocimum gratissimum) readily consumed as food or supplements in many African settings are examined for some of their acclaimed pharmacological activities and possible potential associated risks. The classes of phytochemicals present are examined qualitatively and quantitatively, while the antioxidant activities were determined using three assays which include 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS) and hydrogen peroxide (H2O2) assays. The anti-inflammatory activities were also examined using proteinase inhibitory assays and red blood cell membrane stabilisation assays. Both the antioxidant and anti-inflammatory activities of the plants indicated that the plants have potentials for applications in foods and nutraceuticals that can be used for the management of various ailments and diseases. However, the consumption of H. sabdariffa leaf should be highly regulated as the extract exhibited reduced cell membrane stabilisation activities at increased concentrations.
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    Spectrophotometric determination of amodiaquine in amodiaquine-containing tablets.
    (The Nigeria Association of Pharmacy in Academia., 2025) Abdullahi, S. T., Ogunniyi, O. M., Eniayewu, O. I., Bamidele, O. D., Sanusi, A., Madaki, F. M., Njinga, N. S., & Bakare-Odunola, M. T.; Ogunniyi, O. M.; Eniayewu, O. I.; Bamidele, O. D.; Sanusi, A.; Madaki, F. M.; Njinga, N. S.; Bakare-Odunola, M. T.