Browsing by Author "Azeez Adebola Lateef"

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    Evaluation of the Bio-chemical Markers in Selected Edible Wood Rotting Macrofungi
    (Forestry Association of Nigeria, 2024-05-11) Nusirat Aderinsola Sadiku; Sunday Ademola Laba; Azeez Adebola Lateef; Edidiong Umoren; Isiaka Babatunde Sadiku
    The search for natural products that has therapeutic properties with little or no side effects has been the main subject around the world. Different plants have been and are being explored for their medicinal properties. However, higher fungi remain a vast and a large source of new pharmaceutical products yet to be explored. The phyto-constituents in the ethanolic extract of Six edible wood rotten fungi were investigated by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The results showed that a total of 25 phyto-constituents were revealed in Ganoderma lucidum and Pleurotus florida while 17 compounds were revealed in Pleurotus eryngii and Pleurotus pulmonarius. Pleurotus ostreatus and Pleurotus sajor-caju recorded 31 and 30 phyto-constituents respectively. Some constituents were common to all the fungi while some were absent in some fungi. Generally, most of them occurred in trace amount while a few of the constituents occurred in large amount. Some bioactive compounds such as Methyl stearate, Hexadecanoic acid, methyl ester, (Z)-, Hexadecanoic acid, ethyl ester, 11,14-Octadecadienoic acid, methyl ester and 9-Octadecenoic acid methyl ester were common to all the fungi extract. Four of the fungi had cholesterol content which was however in trace amount of 1.37%, 1.22%, 1.16% and 0.68% for G. lucidum, P. eryngii, P. ostreatus and P. pulmonarius respectively. Of the compounds, 9-Octadecenoic acid, methyl ester, (E)- had the highest concentration of 35.08% in P. pulmonarius. This was closely followed by 11,14-Octadecadienoic acid methyl ester also in P. pulmonarius. Although, the concentration varied across the fungi extracts. The bio-active components in these fungi species could serve as a lead for development of novel natural products with therapeautic properties.
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    Microbial load and molecular chracterization of fungi associated with sweet pepper (Capsicum annuum L.) during storage
    (Published by Institute of Biology, Romanian Academy, 2022) Taofeeq Garuba; Babatunde Yusuf Lawal; Azeez Adebola Lateef; Ganiyu Shittu Olahan
    Peppers are perishable and characterized with a short shelf life. Poor storage methods contribute immensely to their deterioration, leading to a great postharvest loss. This study is therefore aimed at isolation and characterization of fungi causing spoilage in sweet pepper during storage. Some healthy samples of Capsicum annuum fruits were collected from Lasoju farm located in Asa LGA of Kwara State, Nigeria in sterile polyethylene bags and transported to the laboratory. The fruits were kept in the refrigerator, at ambient condition and in an evaporative cooling system. The fruits were observed for spoilage at the end of the second and third week after storage. The spoiled samples were sterilized, cultured on Potato Dextrose Agar, incubated at 25 C for 3–28 days. Thereafter, the different fungal colonies resulted were sub-cultured to obtain pure cultures of each of them. It was revealed that polythene bag as storage material for Capsicum annuum promoted increase in microbial load, while the pure fungal isolates were identified morphologically and molecularly as Aspergillus fumigatus, A. flavus and Rhizopus oryzae. Therefore, the fresh fruits need to be properly handled and stored in order to eliminate or minimize fungal contamination.