Browsing by Author "Ayanniyi, Rashidat"
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Item Characterization, Anti-inflammatory and Anti-microbial Potentials of a Herbal Preparation(Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria, 2019-03) Njinga, Stanislaus; Bakare-Odunola, Mojisola; Mustapha, K.B.; Yahaya, T; Shittu, Abiodun; Bello, H.R; Abdullahi, Sa'ad; Usman, Sukurat; Ayanniyi, Rashidat; Olufadi-Ahmed, HayishaHerbal product (HP) (from Ricinus communis and honey) is used locally for the management of inflammatory and inflammation related disorders including asthma. The current study was carried out to determine the phytochemicals, total phenolic and flavonoid contents, chemical profile, elemental components, anti-inflammatory and antimicrobial activity of the HP used for treatment of asthma. Total phenolic and flavonoid contents were determined by the Folin-Ciocalteus reagent and AlCl3 colorimetric method respectively. The chemo-profiling was done using TLC. Elemental analysis was determined using Atomic Absorption Spectroscopy (AAS). Albumin-induced paw oedema model was used to determine the anti-inflammatory activity while agar well diffusion method was used to determine the antimicrobial activity of the HP at different concentrations. Flavonoids, saponins, alkaloid, cardiac glycoside, steroids and terpenoids were detected in the HP. Total phenolic and flavonoid contents obtained were 10.00 mg/g gallic acid equivalent and 336.60 mg/g quercetin equivalent respectively. The chemo-profiling showed one spot at 254 nm but none at 366 nm. AAS indicated the presence of K, Ca, Fe, Na, Zn, Cu and Pb (Cu and Pb were within WHO acceptable limit). The 100 % concentration produced sudden but transient significant reduction in oedema size at 30 minutes. However, at 60 minutes the effect produced at 25 % was comparable to that of Ibuprofen at 20 mg/kg body weight. At 100% concentration HP all the organisms tested were sensitive except for Pseudomonas aeruginosa. The presence of flavonoids may justify the anti-inflammatory and antimicrobial activities thus validating its use in local treatment of Asthma.Item Evaluation of Nutraceutical content and Topical Anti-inflammatory Activities of Turraea vogelii Hook F. Ex Benth (Meliaceae)(Arak University of Medical Sciences, 2019) Olumoh-Abdul, Hidayah; Ayanniyi, Rashidat; Ojuade, Fatimoh; Agbana, RemilekunBackground: Turraea vogelii has been used as traditional medicine for the treatment of ailments including wounds, stomach aches, malaria, infections, filariasis cutaneous, among others and, it serves as source of food. The present study aimed to evaluate the nutraceuticals content and topical anti-inflammatory effect of hydro-methanol twig extract of Turraea vogelii (METV). Method: METV was obtained using cold maceration with water and methanol in ratio 30:70. The concentration of vitamins A, C and E, total phenolic and total flavonoid contents were evaluated by UV spectrophotometric method. Phenol and xylene-induced ear edema was used to evaluate the topical anti-inflammatory effect in mice. Results: The concentration of vitamins A, C and E was found to be 0.113, 9.303, and 0.020 mg in100 g of dried plant materials, respectively. Total phenolic and flavonoid contents were 0.281 mg/g gallic acid equivalent and 0.112 mg/g quercetin equivalent, respectively. Topical application of METV at a dose of 25 and 50 mg/ear in xylene-induced ear edema produced significant inhibition (P<0.05) of 33 and 54% while in phenol-induced ear edema was significantly (P< 0.05) reduced by 44% and 34%. Dexamethasone (0.1mg/ear) produced an inhibition of 83% and 57% in phenol-induced ear edema. Conclusion: Results from this study demonstrated that Turraea vogelii twigs contain flavonoids and phenolic compounds, which may be responsible for the topical anti-inflammatory effect of the plant extract. Keywords: Anti-Inflammatory Effect, Dexamethasone, Phenol, Topical, Turraea Vogelii Extract, XyleneItem Evaluation of anti nociceptive and anti inflammatory activities of leaf extract of Turraea vogelli Hook. f. ex. Benth(Pakistan Journal of Pharmaceutical Sciences, 2019-01) Ayanniyi, Rashidat; Ojuade, Fatimoh; Olumoh-Abdul, Hidayah; Salawu, Kayode; Olufadi-Ahmed, Haishat; Adebisi-jose, GbemisolaAbstract : The leaf extract of Turraea vogelii Hook. f. ex. Benth . is used in ethnomedicine for the management of pain and inflammation. Anti nociceptive activity was determined using acetic acid induced mouse writhing model. The anti inflammatory activity was investigated using in vitro bovine serum albumin (BSA) denat uration assay and BSA induced hind paw edema in rats. The extract (125 500 mg/kg) administered via the oral route produced a significant ( p <0.005) inhibition of acetic acid induced writhes. The percent inhibition of writhes for extract (500 mg/kg) and dicl ofenac (1 0 mg/kg) was 53.3 and 59.5% respectively. The methanol extract (10 6 1.0 µg /mL) inhibited protein denaturation with IC 50 values of ( 1.06 × 10 3 µg/mL and 2.58 × 10 3 µg/mL) for extract and diclofenac respectively. Furthermore, the leaf extract (62. 5 mg/kg) significantly (p<0.05) inhibited BSA induced paw edema in rats. The methanol leaf extract of T. vogelii has anti nociceptive and anti inflammatory activities. These findings justify the use of the plant in traditional medicine for the management o f pain and inflammation.Item Extractive Values and Diuretic Activity of Methanol Extract of Three Varieties of Hibiscus sabdariffa Linn (Malvaceae) in Wistar Albino Rats(Faculty of Pharmaceutical Sciences, University of Ilorin., 2018-12) Usman, Sukurat; Ayanniyi, Rashidat; Abdullah, AbdulRasheed; Musa, Mariam; Lawal, Bilqis; Salawu, Kayode; Attah, AlfredHibiscus sabdariffa Linn (Malvaceae) is widely cultivated in Nigeria as food and for medicinal purposes. The calyx is the most frequently used part of the plant and has been reported to have antioxidant, antihypertensive and diuretic activity. The aim of the present study is to determine the extractive values and diuretic activity of three varieties of H. sabdariffa. Aqueous, methanol, ethanol, chloroform and hexane soluble extractive values were determined. The effect of the extracts of H. sabdariffa on urine output was evaluated and the concentration of sodium and potassium in blood was also determined. The results showed that water extract had the highest extractive value (32.90%, 26.50% and 29.00%) for the three varieties (Dark red, Light red and Green) respectively. H. sabdariffa extract (100 mg/kg G) produced a significant (p<0.05) increase in urine output compared to the control. The diuretic activity of extract was comparable with the standard drug Hydrochlorothiazide (10 mg/kg). The other species of H. sabdariffa extract produced no significant increase in urine output. H. sabdariffa extract (200 mg/kg G) showed a significant (p<0.05) natriuretic activity compared to control. There was no significance difference in the serum potassium levels for all the three varieties at the doses employed. The study showed that water is the most suitable solvent for extraction of the calyx of H. sabdariffa and the green variety produced a significant increase in urine output with potassium sparing activity.Item Knowledge and Attitude of Guardians towards Eye Health of Primary School Pupils in Ilorin, Nigeria(Nigerian Postgraduate Medical Journal, 2010) Ayanniyi, Abdulkabir; Olatunji, Fatai; Mahmoud, Abdulraheem; Ayanniyi, RashidatObjective: To determine guardians’ knowledge and attitude towards pupils’ eye health and draw implication for designing children-oriented ocular health messages. Methods: A survey of 1,393 guardians selected through multistage random sampling in Ilorin, Nigeria. Using structured questionnaire, information sought included guardians’ demographic characteristics, relationship to pupils, occupation, awareness of eye specialists, perception of normal vision, pupils’ eye complaints, and ways of treating children eye conditions. Data analysis was done with SPSS 12.0.1. The test of significance was performed using Chi square test and significance was taken at p < 0.05. Results: Guardians comprised 689 (49.4%) males and 704 (50.6%) females, mean age 43.61 SD 11.45. Most (88.54%) were pupils’ parents and (11.46%) were relations (n=1,318); (55.15%) were in low-paying occupations (n=1,311); (87.92%) were aware of eye specialists, (12.08%) unaware, (n=1192); (97.27%) adjudged normal vision at least important, (2.73%) not important (n=1,174). Pupils’ eye complaints (n=914) included pain (26.04%), itching (24.73%), redness (21.12%), discharge (8.53%), blur vision (8.21%), photophobia (7.33%) and lacrimation (4.05%). Guardians (n=1,069) managed pupils’ eye diseases using hospital treatment (61.65%), neglect (21.33%), self medication (9.26%) and traditional medication (7.76%). There were no significant associations between guardians’ ways of managing pupils’ eye diseases and their views on normal vision (p = 0.940); awareness of eye care specialists (p = 0.952); and economic occupational grouping (p = 0.959). Conclusion: The negative implications of neglecting eye diseases and use of self and harmful traditional eye-medications by some of the guardians need to be discouraged by appropriate eye health education.Item Neuropharmacological profile of the aqueous leaf extract of Croton zambesicus (euphorbiaceae) in some laboratory animals(Iranian Journal of Pharmacology and Therapeutics., 2008) Ayanniyi, Rashidat; Wannang, NoelTo determine the neuropharmacological activity of the leaf extract of Croton zambesicus in mice and chicks. The effect of aqueous leaf extract of Croton zambesicus on thiopental sodium-induced sleeping time in mice, on gross locomotor activity (GLA) in 2-day old chicks and effect of extract on apomorphineinduced stereotyped behaviour in chicks were evaluated. The aqueous extract (1000 and 1500 mg/kg) administered per os (p.o) prolonged the thiopental sodium-induced sleeping time in mice. Extract (20-40 mg/kg i.p) produced a significant p<0.05 decrease in GLA in 2-day old chicks in a dose dependent manner. 40-60 mg/kg administered intraperitoneally (i.p) produced sedation and sleep with a significant p<0.05 decrease in onset and an increase in duration of sleep. The extract administered subcutaneously (s.c.) had an insignificant p>0.05 effect on apomorphine-induced stereotyped behaviour in chicks. These results suggest that leaf extract of Croton zambesicus possesses CNS depressant, sedative, and hypnotic activity.