Browsing by Author "Attah, A. F"
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Item CHEMICAL DETECTION OF CYSTEINE-RICH CIRCULAR PEPTIDES IN SELECTED TROPICAL VIOLACEAE AND MORINGACEAE FAMILIES USING MODIFIED G-250 AND MASS SPECTROMETRY(Nig. J. Nat. Prod. and Med., 2016) Attah, A. F; Sonibare, M.A.; Moody, J.O.Cysteine-rich circular peptides (CRCs) comprise a large family of gene encoded and low molecular weight polypeptides that has recently engaged the attention of scientists. This class of peptides exhibit a continuous circular configuration and a cystine knot backbone, which defines their resilient naturedirected structural design. Many CRCs have been reported in medicinal plants the first of which is kalata B1 cyclotide from the traditional African plant Oldenlandia affinis. Their detection and isolation can be very challenging and evasive. Only about 1% of plant species have so far been reportedly screened. A modified preliminary chemo-microscopic/macroscopic method involving the use of G-250 stain was applied followed by thin layer chromatographic protosite reaction for plant selection. This was followed by the Matrix Assisted Laser Desorption Time of Flight (MALDI-TOF) Mass spectrometry guided experiment for cyclotide discovery. The blue colouration produced upon sample reaction with modified G-250 led to the selection of potential circular peptide containing plant samples. A further MALDI-TOF MS-guided screening resulted in the detection of circular peptides and cyclotides in Moringa oleifera, Rinorea dentata, R. oblongifolia and R. brachypatela. Viola odorata and Viola tricolor (positive controls) indicated the presence of cyclotides. Results from this study can serve as proof-of-concept for plant selection based on preliminary cysteine-rich circular peptide detection in plants especially with the use of G-250 stain.Item THE EFFECTS OF SOLVENT EXTRACTS OF CITRULLUS LANATUS SEEDS AND CHRYSOPHYLLUM ALBIDUM COTYLEDONS ON RAT HEPATOCYTE REGENERATION, CYTOCHROME C OXIDASE ACTIVITIES AND BACTERIOCINPRODUCING GRAM-NEGATIVE BACTERIA(PharmacologyOnline, 2019-12-30) Molehin, O. R; Ajayi, E. I. O; Attah, A. F; Awope, O. R; Ige, E. T; Omotoyinbo, O. E; Aladetuyi, B. JMedicinal plants have been shown to exert a plethora of biological and pharmacological which has been associated with the presence of several bioactive components. In this study, the effects of crude extracts of Citrullus lanatus seeds (CACL) and Chrysophyllum albidum cotyledons (CACA) on rat hepatocyte regeneration, liver cytochrome c oxidase activities, and their antimicrobial properties on a few bacteriocin-producing Gram-negative bacteria were determined. CACL and CACA were screened for the presence of amino acids using TLC sprayed with ninhydrin, pyridine and modified Commassie brilliant blue (G250). The amino acids were elucidated by Gas Chromatography-Mass Spectrometry (GC/MS) and Fourier-transform infrared spectroscopy (FTIR). CACA enhanced tissue regeneration in rat hepatocytes, in situ and increased the collagen content. CACA also enhanced the activity cytochrome c oxidase in the liver mitochondrial portion, but inhibited same in the homogenate. CACA and CACL did not inhibit the bacteriocin-producing bacteria at the different concentrations tested. It is evident that CACA and CACL contain amino acids that can improve liver antioxidant functions and its mitochondrial electron transport. Both amino acid-rich fractions, combined with bacteriocin may accelerate aseptic wound healing.