Browsing by Author "Atolani, O."
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Item Anti-inflammatory and bronchodilatory constituents of leaf extracts of Anacardium occidentale L. in animal models(Elsevier Limited, Oxford, UK, for Shanghai Association of Integrative Medicine and Shanghai Changhai Hospital, China., 2017) Awakan, J.O.; Malomo, S.O.; Adejare, A.A.; Igunnu, Adedoyin; Atolani, O.; Adebayo, A.H.; Owoyele, B.V.Objective: Anacardium occidentale L. leaf is useful in the treatment of inflammation and asthma, but the bioactive constituents responsible for these activities have not been characterized. Therefore, this study was aimed at identifying the bioactive constituent(s) of A. occidentale ethanolic leaf extract (AOEL) and its solvent-soluble portions, and evaluating their effects on histamine-induced paw edema and bronchoconstriction. Methods: The bronchodilatory effect was determined by measuring the percentage protection provided by plant extracts in the histamine-induced bronchoconstriction model in guinea pigs. The antiinflammatory effect of the extracts on histamine-induced paw edema in rats was determined by measuring the increase in paw diameter, after which the percent edema inhibition was calculated. The extracts were analyzed using gas chromatography-mass spectrometry to identify the bioactive constituents. Column chromatography and Fourier transform infrared spectroscopy were used respectively to isolate and characterize the constituents. The bronchodilatory and anti-inflammatory activities of the isolated bioactive constituent were evaluated. Results: Histamine induced bronchoconstriction in the guinea pigs and edema in the rat paw. AOEL, hexane-soluble portion of AOEL, ethyl acetate-soluble portion of AOEL, and chloroform-soluble portion of AOEL significantly increased bronchodilatory and anti-inflammatory activities (P < 0.05). Oleamide (9-octadecenamide) was identified as the most abundant compound in the extracts and was isolated. Oleamide significantly increased bronchodilatory and anti-inflammatory activities by 32.97% and 98.41%, respectively (P < 0.05). Conclusion: These results indicate that oleamide is one of the bioactive constituents responsible for the bronchodilatory and anti-inflammatory activity of A. occidentale leaf, and can therefore be employed in the management of bronchoconstriction and inflammation.Item CHEMICAL CONSTITUENTS AND ANTIMICROBIAL PROPERTIES OF Phyllanthus amarus (SCHUM & THONN)(Bayero Journal of Pure and Applied Sciences, 2017-06-01) Zubair, M.F.; Atolani, O.; Ibrahim, S.O.; Adebisi, O.O.; Hamid, A.A.; Sowunmi, R.A.The chemical composition of the leave extracts of Phyllanthus amarus (Schum and Thonn) of the family Euphorbiaceae from Nigeria was analyzed by GC-MS. The extracts were also examined for their potential to inhibit the growth of clinical isolates following standard procedure. The major compounds identified in the hexane extract are a flavonoid, flavone 4’,5,7-triethoxy-3,3’,6- trimethoxy (20.23%) and a triterpenoid 17-(1,5-Dimethylhexyl)-6-hydroxy-5-methylestr-9-en-3-yl acetate (19.02%) while bufalin (18.71%) and tetratetracontane (12.91%) were the major compounds detected in the methanol extract. Steroidal triterpenoids are the major compounds present in the extracts as it accounted for 47% of the total detectable content in the hexane extract and 52% in the methanol extract. The steroidal triterpenoids which exist primarily as acetate in the hexane extract include cycloeucalenyl acetate, ergosta-5,7,22-trien-3-ol acetate, macdougallin, 17-(1,5-Dimethylhexyl)-6-hydroxy-5-methylestr-9-en-3-yl acetate, stigmasterol and β-sitosterol while the methanol extract contains 6,7-epoxypregn-4-ene-9,11,18-triol-3,20-dione, 11,18-diacetate, bufalin, olean-13(18)-ene, methyl ursolate, barringenol R1 and 7,8- epoxylanostan-11-ol,3-acetoxy. Hexane extract of the plant exhibited antifungal activity on Candida albicans while methanol extract revealed significant antibacterial activity on Pseudomonas aeruginosa and Staphylococcus aureus at all concentrations of the extract between 12.5 and 100 mg/mL, the activity being comparable to the standard antibacterial drug, Oxacillin. The leaves of Phyllanthus amarus is a potential source of steroidal triterpenoids which could serve as biomarker for the plant species. The extracts of the plant may also serve as a natural source of antimicrobial agents for the treatment of some microbial infections.Item Dietary Oil Type and Late Feed Restriction Elicit Synergistic Effects on Growth, Caeca Bacteria, Carcass, Fat Accretion, and Muscle Lipids in Female Broilers(Wiley & Sons, Inc., 2023) Adeyemi, K.D.; Sola-Ojo, F.E.; El-Imam, A.M.; Atolani, O.; Alli, O.I.; Adegboyega, A.O.; Abubakar, S.O.; Obamonire, O.S.; Ologunade, F.O.The influence of dietary oil type (OT) and late feed restriction (FR) on growth, caeca bacteria count, carcass, separable fat depots, serum and muscle lipids, and meat quality in broilers is examined. In total, 224, 21-day-old female Arbor acres broilers are randomly allotted to either PN, Unrestricted+50 g kg−1 palm oil (PO); PR, 25%-FR+PO; SN, Unrestricted+50 g kg−1 soybean oil (SO); or SR, 25%-FR+SO for 21 d. The SN and PR birds have the heaviest and lightest body weight gain (BWG), respectively. Feed conversion ratio (FCR) is poorer in the PN birds. Feed-restricted birds have more caeca Lactobacillus and lower Salmonella, serum cholesterol, and triglycerides. Carcass yield is higher in SR birds and lower in PN birds. The FR-OT interaction is significant for muscle lipids and weight of fat depots. The PN birds deposit more abdominal, intramuscular, sartorial, mesentery and neck fats, muscle saturated fatty acids, and C18:1n-9 and lower C18:3n-3 and C20:5n-3 than other birds. Neither OT nor FR affects the pH, color, water holding capacity, and oxidative stability of breast meat. The changes elicited by 25%-FR on BWG, FCR, carcass yield, depot fats, and muscle lipids in female broilers are dependent on dietary OT. Practical Application: Fast growth rate and unrestricted access to feed can predispose broiler chickens to excessive fat accretion, which is counterproductive. Producing high-quality carcasses that meet consumers’ expectation is crucial for sustainable and efficient broiler production. The possible interaction between dietary OT and late quantitative FR in mitigating excessive fat accretion and altering production indices in female broilers is explored. Late quantitative FR reduces BWG, FCR, serum lipids, caeca pH and Salmonella spp. and fat accretion in fat depots, and enhances carcass yield and caeca Lactobacillus spp. However, the impacts are more pronounced in the PO birds than the SO birds. Late FR improves the C18:3n-3 and C18:2n-6 contents in PO meat while reducing the C14:0 and C16:0 contents in SO meat. These findings explicate that dietary OT and late quantitative FR can be synergistically used to mitigate excessive fat accretion and alter growth, caeca microflora, and muscle lipids in broilers.Item Isolation, characterization and anti-inflammatory activity of compounds from the Vernonia amygdalina.(Heliyon (Elsevier (Cell Press), Amsterdam, The Netherlands. ), 2024-04) Atolani, O.; Usman, M.A.; Adejumo, J.O.; Ayeni, A.E.; Ibukun, O.J.; Kola-Mustapha, A.T.; Njinga, N.S.; Quadri, L.A.; Ajani, E.O.; Amusa, T.O.; Bakare-Odunola, M.T.; Oladiji, A.T.; Alqahtani, A.; Mohamed, A.; Kambizi, L.Item Proteinease Inhibitory, Membrane Stabilization, Phytochemical and Antioxidant Evaluations of Leaves, Seeds and Calyces of Four Selected Edible Medicinal Plants(Tailoy and Francis, 2017-04-07) Kambizi, L.; Bakare-Odunola, M.T.; Oladiji, A.T.; Kola-Mustapha, A.T.; Amusa, T.O.; Atolani, O.; Njinga, N.S.; Quadri, A.L.The aqueous extracts of the edible parts of four common medicinal plants (Hibiscus sabdariffa, Telfairia occidentalis, Moringa oleifera and Ocimum gratissimum) readily consumed as food or supplements in many African settings are examined for some of their acclaimed pharmacological activities and possible potential associated risks. The classes of phytochemicals present are examined qualitatively and quantitatively, while the antioxidant activities were determined using three assays which include 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzothiazoline- 6-sulfonate (ABTS) and hydrogen peroxide (H2O2) assays. The anti-inflammatory activities were also examined using proteinase inhibitory assays and red blood cell membrane stabilisation assays. Both the antioxidant and anti-inflammatory activities of the plants indicated that the plants have potentials for applications in foods and nutraceuticals that can be used for the management of various ailments and diseases. However, the consumption of H. sabdariffa leaf should be highly regulated as the extract exhibited reduced cell membrane stabilisation activities at increased concentrations.Item Toxicity assessment of sub-acute and sub-chronic oral administration and diuretic potential of aqueous extract of Hibiscus sabdariffa calyces(Heliyon, 2020) Njinga, N.S.; Kola-Mustapha, A.T.; Quadri, A.L.; Atolani, O.; Ayanniyi, R.O.; Buhari, M.O.; Amusa, T.O.; Ajani, E.O.; Folaranmi, O.O.; Bakare-Odunola, M.T.; Kambizi, L.; Oladiji, A.T.; Ebong, P.