Browsing by Author "Animasaun, D.A., Shaibu, B., Oyedeji, S. & Azeez, M.A."

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    Fractionalization of seed storage protein in some selected pea and bean varieties use as food in Nigeria by SDS-PAGE.
    (Annals of Food Science and Technology. Published by Faculty of Environmental Engineering and Biotechnology, Valahia University, Targoviste, Romania, 2017) Animasaun, D.A., Shaibu, B., Oyedeji, S. & Azeez, M.A.
    In this study, nineteen accessions of pea and beans varieties commonly grown for food in Nigeria were assessed for variability and diversity in seed storage protein by sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The electrophorogram revealed presence of protein in the rage of 11-120 kDa in the accessions. Low intra specific heterogeneity was recorded among the accessions, however, inter-specific protein bands was remarkable. Protein fractions with molecular weight of 85 and 120kDa occurred in all the accessions and were also the most abundant. Highest protein fractions were found in accessions NGB/VU/040, NG/OA/11/08/043and NG/SA/07/0098 which are all cowpea varieties, followed by lima bean accessions NG/SA/07/133 and NGB/06/054. Protein fractions of African yam bean accessions were higher than those of pigeon pea and winged bean. At the genetic similarity index of 0.46, the accessions were separated into two main groups, with each group further partitioned into two sub-clusters based on protein band pattern. Accessions with similar protein composition clustered together irrespective of their varietal status. The study concludes that SDS-PAGE analysis is a veritable tool in the assessment of the endospermic proteins in peas and beans. Since seed storage protein is the major determinant of end-use quality of beans in Nigeria, the studied accessions had sufficient protein fractions to justify their dietary use. The genetic diversity and variability of the seed storage proteins obtained in the present study could be useful in identification of protein high yielding varieties for crop improvement and seed production