Browsing by Author "Ameen, M. O."
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Item Anticancer effects of Morinda lucida and Annona muricata on immunomodulations of Melatonin, tumor necrosis factor-alpha and p53 concentrations in lead acetate-induced toxicity in rats(Qassim University, 2021-07) Akinlolu, A. A.; Ameen, M. O.; Oyewopo, A. O.; Kadir, R. E.; Ahialaka, O.; Tijani, S.; Ogungbesan, O.; Bebeyi, R.; Adebayo, S.; Amoo, T.; Abdulazeez, M.Objectives: Lead poisoning accounts for about 0.6% of global burden of disease. Lead-induced toxicity is through confinement of oxidative stress in affected organs. We evaluated the effects of MLF1 (extracted from Morinda lucida leaves) and AMF1 (extracted from Annona muricata leaves) on lipid peroxidation and immunomodulations of Melatonin, tumor necrosis factor-alpha (TNF-α), and p53 proteins in lead acetate (LA)-induced toxicity in rats. Methods: Sixty adult female rats were randomly divided into 12 groups (n = 5). Groups 1 and 2 received physiological saline and 100 mg/kg bodyweight of LA, respectively, for 5 weeks. Groups 3–6 received 100 mg/kg bodyweight LA for 2 weeks, followed by treatments with 7.5 and 15 mg/kg bodyweight of MLF1, and 7.5 and 10 mg/kg bodyweight of AMF1, respectively, for 3 weeks. Groups 7–10 received 7.5 and 15 mg/kg bodyweight of MLF1, 7.5 and 10 mg/kg bodyweight of AMF1, respectively, for 5 weeks. Groups 11–12 received co-administrations of 100 mg/kg bodyweight LA with 15 mg/kg bodyweight MLF1 and 10 mg/kg bodyweight of AMF1, respectively, for 5 weeks. Drugs and extracts were administered orally. Consequently, liver histopathology (Hematoxylin and Eosin), sera Melatonin, and TNF-α (enzyme-linked immunosorbent assay [ELISA]) levels were evaluated. Malondialdehyde (MDA) (thiobarbituric acid assay) and p53 (ELISA) levels were evaluated in liver homogenates. Data were statistically analyzed (P ≤ 0.05). Results: Results showed normal liver histology in all Groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased levels (P ≥ 0.05) of MDA, TNF-α and p53 in Groups 3-12, compared with Group 2. Furthermore, results showed significant (P ≤ 0.05) and non-significant increased Melatonin levels (P ≥ 0.05) in Groups 4–12 compared with Group 2. Conclusion: This study confirmed that MLF1 and AMF1 confer a degree of antioxidant, anticancer and hepato-protetive potentials against LA-induced toxicity in rats.Item The Bioscientist(The Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Enugu State, 2015-01-01) Balogun, K. I.; Arekemase, M. O; Oyeyiola, G. P.; Ameen, M. O.Soil samples collected from engine oil dump sites, diesel polluted sites and uncontaminated plots (controls) were analyzed for oil-degrading and heterotrophic bacteria following standard microbiological and biochemical methods. The pH readings of the different bacterial isolates grown in Mineral Salt Media (MSM) for 16 days, Optical density readings, enumeration of bacterial isolates in the different MSM were used as indices for comparison of the rate of biodegradation of the diesel types.Nine indigenous microorganisms were isolated from both the engine oil and petrodiesel contaminated soils using the enrichment technique. Oil degraders isolated include: Pseudomonas aeruginosa, Pseudomonas cepacia, Staphylococcus schleiferi, Pseudomonas fluorescens, Bacillus subtilis, Klebsiellapneumoniae, Staphylococcus chromogenes, Escherichia coli and Micrococcus varians.Heterotrophic bacterial counts were significantly higher (P < 0.05) in non-impacted than in impacted soils. Conversely, the population of oil degraders was significantly lower in non-impacted than in impacted soils. The pH range was 3.90-5.70 while the absorbance at 600nm range was 0.10 -1.17. The bacterial plate counts in MSM supplemented with biodiesel were higher than in those supplemented with biodiesel blend and petrodiesel. Results show that biodiesel is more easily and faster biodegraded than petrodiesel. This finding could be exploited in case of oil-spill clean-up campaigns.Item Corrosion inhibition efficacy of hybrid organic extracts from prosopisafricana and citrulluslanatus on mild steel in acidic medium(Faculty of Engineering, “Dunarea De Jos” University of Galati Romania, 2023-03-15) SHUAIB-BABATA, Yusuf Lanre; Ayinde, I. V.; Busari, Y. O.; Ameen, M. O.; Yahya, R. A; Ambali, I. O.; Aremu, N. I.; Abdulraman, S. O; Ajao, K. S; Garuba, A. A.Each of Prosopis Africana pod extract (PAPE) and Citrulluslanatus (WMPE) performed quite well as green inhibitors in the electrochemical corrosion in acidic solution at ambient temperature in previous research. However, the hybridization of these compounds was varied with the concentration ratio in this study to optimize the efficiency of the green inhibitor in a hydrochloric acid medium using mild steel. The extraction process of the inhibitor samples was carried out with the Soxhlet apparatus using n-hexane as the solvent. Furthermore, the potential inhibition efficiency was monitored using various corrosion measurement techniques at room temperature. The inhibitive response of the extracts could be attributed to the adsorption of the extracts’ components on mild steel surface by physisorption mechanism according to the Langmuir adsorption isotherm model. The results revealed that inhibition efficiency (IE%) depends on the concentration of the extracts. The peak IE% values were obtained with a hybrid ratio of 3:1 (PAPE: WMPE) for gravimetric technique. The Tafel polarization and gasometrical measurement technique is 96.7%, 98.5% and 83.1% respectively at the concentration of 1.0 g/L, which are above the peak IE% for PAPE (93.7%, 80.96% and 77.8%) and WMPE (92.7%, 80.5% and 75.6%)Item The effects of Moringa Oleifera on lipid profile status, heart histology, and liver histochemistry in adult Wistar rats(Wolters Kluwer - Medknow, 2017-04-10) Akinlolu, A. A.; Bayode, E. O.; Ghazali, K. O.; Ameen, M. O.Background: Moringa oleifera’s leaves are globally used for nutritional and medicinal purposes. We evaluated lipid profile status, heart and liver histology, and the activities of alanine and aspartate transaminases of the liver in M. oleifera‑treated adult Wistar rats. Materials and Methods: Twenty‑four adult male Wistar rats were employed in the study. Rats of control Group I received physiological saline while rats of Groups II–IV received 250, 500, and 750 mg/kg bodyweight of methanolic extract of M. oleifera, respectively, for 21 days. Results: No gross morphological or behavioral anomalies were observed in rats of Groups I–IV. Histological examinations showed normal histoarchitecture of the heart in Group I and the liver of Groups I–III. Comparative histological analyses showed dose‑dependent increased nucleated cells in heart tissues of rats of Groups II–IV than those of Group I. Furthermore, histological evaluations showed hypertrophy of central vein of the liver in rats of Group IV when compared to Group I. Statistical analyses showed dose‑dependent non significant higher mean levels (P > 0.05) of total cholesterol and triglycerides in the sera of rats of Groups II–IV compared to rats of Group I. Evaluations of levels of alanine and aspartate transaminases showed a statistically significant higher mean values (P < 0.05) in Group II, but statistically nonsignificant higher mean values (P > 0.05) in Groups III–IV compared to Group I. Conclusions: No significant adverse effects on lipid profile status, heart histology, and liver histochemistry were observed in M. oleifera‑treated rats.Item Moringa oleifera and Musa sapientum ameliorated 7,12-Dimethylbenz[a]anthracene-induced upregulations of Ki67 and multidrug resistance 1 genes in rats(Qassim University, 2021-05) Akinlolu, A. A.; Oyewopo, A. O.; Kadir, R. E.; Lawal, A.; Ademiloye, J.; Jubril, A.; Ameen, M. O.; Ebito, G. E.Objectives: Moringa oleifera (MO) and Musa sapientum (MS) are plants of ethnomedicinal importance. We evaluated the effects of MOF6 (extracted from MO leaves) and MSF1 (extracted from MS suckers) on immunomodulations of Ki67 (proliferation biomarker) and multidrug resistance 1 (MDR1) genes in the liver of rats in 7,12-Dimethylbenz[a]anthracene (DMBA)-induced hepatotoxicity and mutagenesis to determine their antiproliferation, anti-drug resistance, and anticancer potentials. Methods: Forty-five adult male rats were randomly divided into nine groups (n = 5). Groups 1 and 2 received physiological saline and 15 mg/kg bodyweight of DMBA, respectively. Groups 3 and 4 received 15 mg/kg bodyweight DMBA and were treated with 15 and 30 mg/kg bodyweight of MOF6, respectively. Group 5 received 15 mg/kg bodyweight DMBA and was treated with 10 mg/kg bodyweight of MSF1. Group 6 received 15 mg/kg bodyweight DMBA and was treated with 3.35 mg/kg bodyweight of doxorubicin and intravenous injection of 0.5 ml/200 g of cisplatin. Groups 7–9 received only 15 and 30 mg/kg bodyweight of MOF6 and 10 mg/kg bodyweight of MSF1, respectively. DMBA, doxorubicin, and extracts doses were administered orally. The duration of our experimental procedure was 8 weeks. Consequently, liver histopathology (hematoxylin and eosin technique) and enzyme-linked immunosorbent assay homogenates’ concentrations of Ki67 and MDR1 were evaluated. Computed data were statistically analyzed (P ≤ 0.05). Results: Results showed normal histoarchitectures of the liver in all groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased concentrations (P ≥ 0.05) of Ki67 and MDR1 in Groups 3–9 compared with Group 2. Therefore, MOF6 and MSF1 ameliorated DMBA-induced hepatotoxicity, abnormal proliferation, and drug resistance. Conclusion: MOF6 and MSF1 possess antiproliferation, anti-drug resistance, and anticancer potentials.Item Nig. J. Nat. Prod. and Med.(Nigerian Society of Pharmacognosy, 2015-05-01) Owoyele, B. V.; Abioye, A. I. R.; Oyewole, A. L.; Ameen, M. O.; Owemidu, I. O.; Adewusi, M. O.Bulbostylis coleotricha (family Cyperaceae) is a stem-tufted plant found in tropical region of Africa. It is locally used in treating various ailments among the African folks. Despite the high esteem this plant is held in Africa, there is dearth of scientific studies on B. coleotricha. This, in addition to the alarming increase in inflammatory diseases around the world, makes the present study to focus on the effect of the B. coleotricha extract on laboratory induced inflammation. Twenty-five adult Wistar rats, weighing between 225-287g were used for this experiment, and were randomly divided into five groups of five rats per group. The groups were labelled and treated as follow: Group 1, the negative control group, was treated with 10 ml/kg body weight (b. w.) of distilled water.Groups 2, 3 and 4, the treated groups, were treated with 25 mg, 50 mg and 100 mg/kg b. w. of ethanolic extract of B. coleotricha respectively. Doses were based on the local information on B. coleotricha usages. Group 5, the positive control group, was treated with 5mg/kg b. w. of indomethacin. In all the groups, the effects of all treatments were checked on induced inflammation using cotton pellet granuloma and carrageenan induced paw oedema model.The results in the two models used showed that ethanolic extract of B. coleotricha significantly inhibited inflammation in the treated groups. In the cotton pellet granuloma test the extract at the dose of 100 mg reduced granuloma formation from 30.2 ± 2.4 mg to 11.2 ± 2.6 mg reduced paw oedema from 7.0 ± 1.7 mm to 2.4 ± 0.8 mm (control is 100 mg/kg). In conclusion, this study established that Bulbostylis coleotricha plant is effective in the treatment of inflammation and can be explored in the treatment of various diseases with underlying inflammationItem Performance and Corrosion Inhibitive Mechanism of Prosopis Africana Pod Extracts on Mild Steel in Acidic Medium(Faculty of Engineering, University of Port Harcourt, Nigeria, 2022) Ayinde, I. V.; Ameen, M. O.; Busari, Y. O.; Ambali, I. O.; Abdulraman, S. O.; Ajao, K. S.This study investigates the influence of Prosopis Africana pod extract (PAPE) on corrosion behavior of mild steel in 1.0 M HCl. The Prosopis African Seed Extract (PASE) was previously characterized as a green corrosion inhibitor without considering its pod, which often constitutes environmental pollution. The PAPE solution was administered in varying concentrations of 0.0 g/ml to 10.0 g/ml as a green inhibitor of mild steel in 1M Hydrochloric (HCl) medium at room temperature using gravimetric, gasometric and electrochemical (Tafel Polarization) techniques. The volume of hydrogen evolution determined with gasometric process decreases as the concentration of the extract increases. The gravimetric technique recorded the highest inhibition efficiency (IE%) of 97.14% with a 1.0g/l concentration of PAPE. The electrochemical measurements of the corrosion current density (ICorr) decreased as the concentration of PAPE increased, with a maximum IE% of 80.59% (PAPE) at 1.0g/l recorded. Generally, IE% increases with the concentration of the extract. The decrease in the corrosion rate is caused by the intensified obstructive mechanism in the coupon surfaces instigated by the pod extract's adsorption. Hence, the extract from Prosopis Africana Pod is equally suitable as a green inhibitor for corrosion.Item Research Journal of Health Sciences(2018-02-01) Omotoso, G. O.; Kadir, R. E.; Gbadamosi, I. T.; Akinlolu, A. A.; Adunmo, G. O.; Kolo, R. M.; Lawal, M. O.; Ameen, M. O.Objectives: Cuprizone is a neurotoxicant used in modeling demyelinating disorders. This study explored the effects of Moringa oleifera (MO) on oxidative, histomorphological and behavioural changes in cuprizone-damaged cerebellum. Methods: Twenty adult female Wistar rats were grouped into 4, each group having five animals. Group A received 1 ml of normal saline (Control); group B received 0.4% cuprizone; group C received 15.6 mg/kgBW Moringa oleifera leaf extract; group D received 0.4% cuprizone and 15.6 mg/kgBW Moringa oleifera, orally for 5 weeks. The animals were assessed for exploratory and locomotor activities, while the cerebellum was processed for histology and assayed for nitric oxide (NO), catalase (CAT) and superoxide dismutase (SOD) activities. Results: Cuprizone treatment caused weight reduction, disruption of Purkinje cell layer, cellular degeneration, reduction in NO, CAT and SOD activities. However, these changes were ameliorated when co-administered with MO. Conclusion: The anti-oxidative property of Moringa oleifera is responsible for its ameliorative effect in cuprizone neurotoxicity.