Browsing by Author "Alabi, Ade Stephen"

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    Cigarette Smoke alters Testicular and Epididymal Histology in Adult Wistar Rats.
    (Journal of Experimental and Clinical Anatomy,, 2017) Omotoso, Gabriel Olaiya; Hambolu, O Zoe; Alabi, Ade Stephen
    Background: Cigarette smoking is a major public health problem. Different disease conditions have been linked to smoking as a risk factor, including infertility, with most studies focusing on semen quality. Aim: The current study aimed at determining the effects of cigarette smoking on the histology of both the testes and epididymis in animal models. Materials and Methods: Sixteen adult male Wistar rats were grouped into four groups: Control Group A and 3 treated Groups B, C, and D, exposed to 1, 2, and 3 sticks of cigarette, respectively. Smoking chambers, made of cylindrical containers, were constructed indigenously and used for the exposure procedure. Each stick was suspended within the chamber and allowed to completely burn for about 10 min. Cigarette exposure was carried out for 28 consecutive days. Both the testes and caudal epididymis were examined histologically. Results: There was distortion of the testicular and epididymal architecture and varying degrees of structural degeneration, especially in the group exposed to the highest concentration of cigarette smoke; spermatogenic cells appeared to decrease in population and there was a reduction in the density of mature spermatozoa in the lumen of the epididymis and seminiferous tubules. Conclusion: The architectural changes in the testes and epididymis explain the reasons for low or poor semen quality in subjects exposed to cigarette smoke, and possible impaired reproduction.
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    Effects of unripe Musa paradisiaca on the histochemistry of the testis and testosterone levels in adult albino rats.
    (Nigerian Journal of Physiological Sciences,, 2017) Alabi, Ade Stephen; Omotoso, Gabriel Olaiya; Tagoe, CNB; Akinola, OB; Enaibe, BU
    Summary: This study was aimed at determining the effects of the unripe fruit of Musa paradisiaca on the testis and testosterone levels in male Wistar rats. The animals were grouped into three, comprising a control, and 2 treatment groups administered with different doses (500 mg/kg and 1000 mg/kg) daily of the fruit flour over 28 days. Histochemical evaluation of the testes was done using Haematoxylin and Eosin, Periodic acid Schiff’s (PAS) and Feulgen staining techniques, while the serum and homogenised testicular tissue were evaluated for testosterone levels using Accu-Bind ELISA Kit. The testis of the treated groups showed more rapidly dividing cells and more population of sperm cells compared to the control group, and also showed more positivity for Feulgen staining and PAS reaction. Both serum and testicular testosterone levels were however reduced. Serum testosterone was significantly lowered in the animals given the low dose (0.67 ± 0.03 ng/ml), compared to those given high dose (0.85 ± 0.02 ng/ml) and the control animals (1.88 ± 0.15 ng/ml) (p < 0.05). Changes in testicular testosterone were not statistically significant. The study suggests that M. paradisiaca fruit has reproductive enhancing potential when consumed moderately, but this benefit may not be related to testosterone levels.