Browsing by Author "Akinola, Olugbenga"

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    EFFECTS OF WHOLE CANNABIS SATIVA INGESTION ON BEHAVIOURAL PATTERNS AND OXIDATIVE STRESS IN MICE BRAIN TISSUES
    (Animal Research International, 2019-04-12) Akinola, Olugbenga; Olumoh-Abdul, Hidayah Ayodeji; Oyewole, L. Aboyeji; Owolodun, O. Abibat; Egboro, D. Efe; Abdulkadir, B. Fatima; Iyenmoana, Ese; Yakub, Y. Olatunji; Akinyele, Akinsola; Ogbeche, O.Elizabeth
    The unregulated habitual use of whole Cannabis sativa remains a challenge for the potential medical usefulness of the plant. As a psychoactive substance with different physiological properties, the onset and extent of its effects are often a factor of the mode of consumption. This study evaluated the neuro-behavioural effects of daily oral ingestion of C. sativa and its modulatory changes in oxidative stress parameters in mice brain tissues. Twenty-five male Swiss albino mice were separated into 5 groups of 5 animals each. Cannabis-diet were prepared from whole dried cannabis and standard mice feed. Groups I – IV, were fed with 40, 20, 10 and 1 % cannabis-diet ad libitum for 14 days, while group V animals were fed the standard mice diet ad libitum for 14 days and served as control. Neuro-behavioural activities were assessed by observing animals rearing, grooming, ambulation, head dipping and freezing times. The brain oxidative stress parameters were assayed to determine the effect of cannabis oral consumption on activity in mice brain. The animals fed with cannabis-diet displayed significantly reduced anxiety but statistically insignificant locomotory function, exploratory tendencies and neophilia, in a quantity dependent manner relative to the controls. Cannabis demonstrated both antioxidant and oxidative stress tendencies. Ingestion of whole cannabis plants may not adversely influence neuro-behavioural patterns in animals. A trade-off between oxidative stress induction and brain tissue injury repair mechanisms may have been elicited by different constituents of Cannabis. Thus, oral ingestion of cannabis may not readily cause changes in neuro-behavioural patterns.
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    Oral Ingestion of Cannabis sativa: Risks, Benefits, and Effects on Malaria-Infected Hosts
    (Cannabis and Cannabinoid Research, 2018-11-22) Akinola, Olugbenga; Ogbeche, O.Elizabeth; Olumoh-Abdul, H.A; Alli-Oluwafuyi, O. Abdulmusawwir; Oyewole, L. Aboyeji; Amin, Abdulbasit; AbdulMajeed, I. Wahab; Olajide, J. Olayemi; Nafiu, B. Abdurrazaq; Njan, A. Anoka; Olorundare, E. Olufunke; Gbotosho, O. Grace
    Background: The emergence of a multidrug-resistant strain of Plasmodium falciparum (Pf Pailin) raises concern about malaria control strategies. Unfortunately, the role(s) of natural plants/remedies in curtailing malaria catas￾trophe remains uncertain. The claims of potential antimalarial activity of Cannabis sativa in vivo have not been well established nor the consequences defined. This study was, therefore, designed to evaluate the effects of whole cannabis consumption on malaria-infected host. Methods: Thirty mice were inoculated with dose of 1 · 107 chloroquine-resistant Plasmodium berghei ANKA￾infected erythrocyte and divided into six treatment groups. Cannabis diet formulations were prepared based on weighted percentages of dried cannabis and standard mice diet and the study animals were fed ad libitum. Chemosuppression of parasitemia, survival rates, parasite clearance, and recrudescence time were evaluated. His￾topathological studies were performed on the prefrontal cortex (PFC) and hippocampus of the animals after 14 days’ consumption of cannabis diet formulation by naive mice. Results: There was a significant difference ( p < 0.05) in the day-4 chemosuppression of parasitemia between the animals that were fed C. sativa and chloroquine relative to the untreated controls. There was also a significant difference in the survival rate ( p < 0.05) of animals fed C. sativa diet (40%, 20%, 10%, and 1%) in contrast to control animals on standard mice diet. A parasite clearance time of 2.18 – 0.4 was recorded in the chloroquine treatment group, whereas recrudescence in chloroquine group occurred on day 7. There were slight histomorphological changes in the PFC and cell densities of the dentate gyrus of the hippocampus of animals that were fed C. sativa. Conclusions: C. sativa displayed mild antimalarial activity in vivo. There was evident reduction in symptomatic man￾ifestation of malaria disease, though unrelated to levels of parasitemia. This disease tolerance status may be beneficial, but may also constitute a transmission burden through asymptomatic carriage of parasites by habitual cannabis users.